Date published: 2025-9-7
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p-Pol II Antibody (8A7) - Western Blotting - Image 1259
p-Pol II 抗体 (8A7): sc-13583. 未经处理 (A), 热休克 (C) 和钙culin 处理 (E) 的 HeLa 培养物中 Pol II 磷酸化的 Western 印迹分析. 使用 Pol II (A-10): sc-17798 测定相同提取物中 Pol II 的总含量 (B, D, F).
p-Pol II 抗体 (8A7): sc-13583. 未经处理 (A), 热休克 (C) 和钙culin 处理 (E) 的 HeLa 培养物中 Pol II 磷酸化的 Western 印迹分析. 使用 Pol II (A-10): sc-17798 测定相同提取物中 Pol II 的总含量 (B, D, F).

p-Pol II 抗体 (8A7): sc-13583

4.9(7)
寫評論提問

说明书
  • p-Pol II抗体(8A7)是小鼠单克隆IgM κ, 在26篇文献中引用，规格为200 µg/ml
  • 针对含有human来源的p-Pol II的磷酸化Ser 1801的合成肽
  • 推荐用于 Ser 1801 phosphorylated RNA polymerase II of human origin and correspondingly phosphorylated RNA polymerase II of mouse, rat and S. cerevisiae origin WB, IP, IF 和 IHC(P)检测
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13583 X, 200 µg/0.1 ml)
  • m-IgGκ BP-HRP 是 p-Pol II 抗体 (8A7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与p-Pol II 抗体 (8A7) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外抗体，包括RNA pol α, Pol II, Pol II RPB5, Pol II RPB6, Pol III RPC32, Pol III RPC39, RNA pol σ, RPA135, RPA16和RPA194

RNA聚合酶II（Pol II）是一种多亚基酶，负责蛋白质编码基因的转录。转录起始需要通过激活因子和染色质重塑因子的诱导将完整的转录机制招募到启动子。 Pol II可以协调10到14个亚基。这个复合体与基因的启动子区域和各种元件和转录因子相互作用。聚合酶的DNA结合结构域是一个凹槽，TFIIB在此定位DNA以解旋和转录。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Pol II 抗体 (8A7) 参考文献:

  1. 磷酸化RNA聚合酶II刺激前mRNA剪接。  |  Hirose, Y., et al. 1999. Genes Dev. 13: 1234-9. PMID: 10346811
  2. 转录的结构基础：4.5 埃的 RNA 聚合酶 II-TFIIB 共晶体。  |  Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
  3. RNA 聚合酶 II 的结构和功能。  |  Cramer, P. 2004. Adv Protein Chem. 67: 1-42. PMID: 14969722
  4. RNA 聚合酶 I 的转录机制。  |  Comai, L. 2004. Adv Protein Chem. 67: 123-55. PMID: 14969726
  5. RNA 聚合酶 II 的转录周期：在染色质中循环。  |  Svejstrup, JQ. 2004. Biochim Biophys Acta. 1677: 64-73. PMID: 15020047
  6. RNA 聚合酶 III 转录与癌症  |  White, RJ. 2004. Oncogene. 23: 3208-16. PMID: 15094770
  7. BRCA1 与 NUFIP 和 P-TEFb 合作，激活 RNA 聚合酶 II 的转录。  |  Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
  8. 表皮生长因子可提高细胞中 TATA 结合蛋白的水平，并诱导 RNA 聚合酶 I 和 III 依赖性基因的活性。  |  Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
  9. 视网膜母细胞瘤肿瘤抑制蛋白抑制 RNA 聚合酶 III 转录的不同机制  |  Hirsch, HA., et al. 2004. Mol Cell Biol. 24: 5989-99. PMID: 15199152
  10. 下游 DNA 选择性地影响人类 RNA 聚合酶 II 的暂停构象。  |  Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834

订购信息

产品名称产品编号规格价格数量收藏夹

p-Pol II 抗体 (8A7)

sc-13583
200 µg/ml
RMB2377.00

p-Pol II (8A7): m-IgGκ BP-HRP 套装

sc-551922
200 µg Ab; 40 µg BP
RMB2662.00

p-Pol II 抗体 (8A7) X

sc-13583 X
200 µg/0.1 ml
RMB2377.00

When should the TransCruz reagent sc-13583 X be used instead of the standard form sc-13583?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from normal useDuring normal use, the traits in WB are good. The mutation state in the reference object is not obvious
Date published: 2017-01-18
Rated 4 out of 5 by from Pretty good antibodyPretty good antibody. We have used a different lot in the past and it worked really well. No big complaints.
Date published: 2015-06-19
Rated 5 out of 5 by from Use it routinelyUse it routinely, works well in WB, no complaints
Date published: 2015-04-21
Rated 5 out of 5 by from Strong nuclear localization in IF in methanolStrong nuclear localization in IF in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Worked well in IF in monkey CVWorked well in IF in monkey CV-1 cells. -SCBT Publication Review
Date published: 2015-03-05
Rated 5 out of 5 by from Good antibody for ChIP in human HEK293Good antibody for ChIP in human HEK293, MCF-7 cells. -SCBT Publication Review
Date published: 2015-01-14
Rated 5 out of 5 by from Strong band in WB in HeLa whole cell lysatesStrong band in WB in HeLa whole cell lysates. -SCBT QC
Date published: 2013-08-20
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p-Pol II 抗体 (8A7) is rated 4.9 out of 5 by 7.
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