Date published: 2025-9-6
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p-PKC beta 2/delta Antibody (E-7) - Western Blotting - Image 141605
未经处理 (A, C) 和经λ蛋白磷酸酶 (sc-200312A) 处理 (B, D) 的 HeLa 全细胞裂解液中 PKC βIl/δ 磷酸化的 Western 印迹分析. 测试的抗体包括 p-PKC βII/δ (E-7): sc-365463 (A, B) 和 PKC δ (C-20): sc-937 (C, D).
未经处理 (A, C) 和经λ蛋白磷酸酶 (sc-200312A) 处理 (B, D) 的 HeLa 全细胞裂解液中 PKC βIl/δ 磷酸化的 Western 印迹分析. 测试的抗体包括 p-PKC βII/δ (E-7): sc-365463 (A, B) 和 PKC δ (C-20): sc-937 (C, D).

p-PKC beta 2/delta 抗体 (E-7): sc-365463

4.8(6)
寫評論提問

说明书
  • p-PKC beta 2/delta抗体(E-7)是小鼠单克隆IgG2a κ, 在6篇文献中引用，规格为200 µg/ml
  • 针对含PKC βII的human起源的磷酸化Ser 660的氨基酸序列
  • p-PKC beta 2/delta 抗体 (E-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测 Ser 660 phosphorylated PKC βII of mouse, rat and human origin, correspondingly Ser 662 phosphorylated PKC δ of mouse and rat origin, and correspondingly Ser 664 phosphorylated PKC δ of human origin
  • 抗p-PKC beta 2/delta抗体（E-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-PKC beta 2/delta (E-7): sc-365463的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 是p-PKC beta 2/delta 抗体 (E-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与p-PKC beta 2/delta 抗体 (E-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
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研究信息

関連項目

此外 PKC 抗体包括 PKC α, PKC β, PKC δ, PKC ε, PKC η, PKC γ, PKC ι, PKC λ, PKC μ 和 PKC ν

p-PKC βII/δ 抗体（E-7）是一种 IgG2a κ 小鼠单克隆 p-PKC βII/δ 抗体（也称 PKC beta 2/delta 抗体），可通过 WB、IP、IF、IHC(P) 和 ELISA 检测小鼠、大鼠和人源的 Ser 660 磷酸化 PKC βII、小鼠和大鼠源的相应 Ser 662 磷酸化 PKC δ 以及人源的相应 Ser 664 磷酸化 PKC δ。p-PKC βII/δ 抗体 (E-7) 可提供非结合型抗 p-PKC βII/δ 抗体，以及多种结合型抗 p-PKC βII/δ 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 结合物。蛋白激酶 C（PKC）家族成员在细胞生长和分化、基因表达、激素分泌和膜功能等多种细胞功能中发挥着关键的调节作用。PKC 最初被鉴定为丝氨酸/苏氨酸蛋白激酶，其活性依赖于钙和磷脂。二酰甘油（DAG）和肿瘤促进剂磷脂与 PKC 结合并激活 PKC。PKC 至少可细分为两大类，包括传统（c）PKC 同工酶（α、βI、βII 和 γ）和新型（n）PKC 同工酶（δ、ε、ζ、η 和 θ）。每种 PKC 异构体在不同组织中的表达模式各不相同，PKC 家族成员对辅助因子的依赖性也明显不同。例如，nPKC δ 和 ε 的激酶活性与 Ca2+ 无关。另一方面，nPKC δ 和 ε 以及所有 cPKC 成员都具有磷酸酯结合活性和激酶活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p-PKC beta 2/delta 抗体 (E-7)

sc-365463
200 µg/ml
RMB2377.00

p-PKC beta 2/delta (E-7): m-IgG2a BP-HRP 套装

sc-545939
200 µg Ab; 10 µg BP
RMB2662.00

p-PKC beta 2/delta 抗体 (E-7) AC

sc-365463 AC
500 µg/ml, 25% agarose
RMB3129.00

p-PKC beta 2/delta 抗体 (E-7) HRP

sc-365463 HRP
200 µg/ml
RMB2377.00

p-PKC beta 2/delta 抗体 (E-7) FITC

sc-365463 FITC
200 µg/ml
RMB2482.00

p-PKC beta 2/delta 抗体 (E-7) PE

sc-365463 PE
200 µg/ml
RMB2580.00

p-PKC beta 2/delta 抗体 (E-7) Alexa Fluor® 488

sc-365463 AF488
200 µg/ml
RMB2685.00

p-PKC beta 2/delta 抗体 (E-7) Alexa Fluor® 546

sc-365463 AF546
200 µg/ml
RMB2685.00

p-PKC beta 2/delta 抗体 (E-7) Alexa Fluor® 594

sc-365463 AF594
200 µg/ml
RMB2685.00

p-PKC beta 2/delta 抗体 (E-7) Alexa Fluor® 647

sc-365463 AF647
200 µg/ml
RMB2685.00

p-PKC beta 2/delta 抗体 (E-7) Alexa Fluor® 680

sc-365463 AF680
200 µg/ml
RMB2685.00

p-PKC beta 2/delta 抗体 (E-7) Alexa Fluor® 790

sc-365463 AF790
200 µg/ml
RMB2685.00

p-PKC beta 2/delta (E-7) 中和勝肽

sc-365463 P
100 µg/0.5 ml
RMB511.00

Dear Madam/Sir, Does sc-365463 antibody recognize also alpha-PKC isoform when phosphotylated at residue corresponding to Ser660 of PKC beta II?

Asked by: Matthew2
Thank you for your question. p-PKC βII/δ Antibody (E-7): sc-365463 recognizes Ser 660 phosphorylated PKC βII (mouse, rat, or human origin), Ser 662 phosphorylated PKC δ (mouse or rat origin), and Ser 664 phosphorylated PKC δ (human origin) by WB, IP, IF, IHC(P) and ELISA. These two antibodies are recommended for the detection of p-PKC α: p-PKC α Antibody (A-11) sc-377565 https://www.scbt.com/scbt/product/p-pkc-alpha-antibody-a-11 p-PKC α Antibody (pT638.35) sc-517540 https://www.scbt.com/scbt/product/p-pkc-alpha-antibody-pt638-35
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Does the antibody detect both phosphorylated PKC beta and delta?

Asked by: Dhanalakshmi
Yes, this antibody is recommended for detection of Ser 660 phosphorylated PKC βII of mouse, rat and human origin, correspondingly Ser 662 phosphorylated PKC δ of mouse and rat origin, and correspondingly Ser 664 phosphorylated PKC δ of human origin.
Answered by: Technical Support
Date published: 2018-12-21

What application is the blocking peptide sc-365463 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from gutThe antibody worked very well at 1:500 dilution on THP-1 cells stimulated with PMA but the delta band is more or less absent.
Date published: 2019-03-05
Rated 5 out of 5 by from perfect antibodyperfect results with specific band for target protein
Date published: 2017-12-04
Rated 5 out of 5 by from works great on western blot and IHCWe have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 5 out of 5 by from Very potent and specific antibodyThis monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is highly recommended for Western blotting. There was very strong detection of ~84 kDa protein that may be PKC-beta in human HeLa cells treated for 2 hours with anisomycin on Western blots, but no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2016-12-04
Rated 5 out of 5 by from Works well for IF applicationsWorks well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-01-09
Rated 5 out of 5 by from Expected results obtained by Western blotExpected results obtained by Western blot analysis of PKC Il/ phosphorylation in untreated and lambda protein phosphatase treated HeLa whole cell lysates. -SCBT QC
Date published: 2014-03-11
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p-PKC beta 2/delta 抗体 (E-7) is rated 4.8 out of 5 by 6.
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