Date published: 2026-10-24

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p-eNOS Antibody (15E2) - Western Blotting - Image 62936
p-NOS3 抗体 (15E2): sc-81510. 未经处理 (A) 和经巴伐那酸处理 (B) 的弯曲 3 全细胞裂解液中 NOS3 磷酸化的 Western 印迹分析.
p-eNOS Antibody (15E2) - Western Blotting - Image 62936
p-NOS3 抗体 (15E2): sc-81510. 未经处理 (A) 和经巴伐那酸处理 (B) 的弯曲 3 全细胞裂解液中 NOS3 磷酸化的 Western 印迹分析.
p-NOS3 抗体 (15E2): sc-81510. 未经处理 (A) 和经巴伐那酸处理 (B) 的弯曲 3 全细胞裂解液中 NOS3 磷酸化的 Western 印迹分析.

p-eNOS 抗体 (15E2): sc-81510

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说明书
  • p-eNOS 抗体 15E2 是小鼠单克隆 IgG1, 在13篇文献中引用，提供 50 µg/0.5 ml
  • 与humanPARP-1的NOS3中丝氨酸1177周围的氨基酸残基对应的合成磷酸化肽
  • 推荐用于 mouse, rat 和 human 来源的Ser 1177 phosphorylated NOS3 WB 和 IP检测
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是p-eNOS Antibody (15E2) for WB applications. 的首选辅助检测试剂，这些试剂现与p-eNOS Antibody (15E2) 打包提供（请参阅下面的订购信息）。
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附加NOS抗体，包括NOS1, NOS2, NOS3和pan NOS

p-NOS3抗体（15E2）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测小鼠、大鼠和人类样本中Ser 1177磷酸化NOS3。抗磷酸化NOS3抗体（15E2）以非结合形式提供，为各种实验设置提供了灵活性。NOS3，也称为内皮一氧化氮合酶，通过合成一氧化氮（NO）在心血管健康中发挥关键作用。一氧化氮是一种重要的信号分子，可调节血管张力、血压和血管生成。NOS3的正常运作有助于维持心血管的稳态；失调会导致各种心血管疾病。NOS3通过丝氨酸1177的磷酸化激活，这一过程由丝氨酸/苏氨酸蛋白激酶Akt/PKB介导，而丝氨酸/苏氨酸蛋白激酶Akt/PKB是磷脂酰肌醇3-激酶（PI 3-kinase）的重要下游靶标。这种磷酸化反应对于内皮细胞响应血管内皮生长因子（VEGF）等生长因子而迁移至关重要，凸显了NOS3在血管生成和组织修复中的重要性。p-NOS3 (15E2) 单克隆抗体使研究人员能够有效地研究NOS3的激活状态及其在各种生理和病理过程中的意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-eNOS 抗体 (15E2) 参考文献:

  1. 机械活动激活一氧化氮合成酶（NOS3）以Ca2+独立的方式改变心肌细胞的收缩活动：肌钙蛋白I磷酸化作用。  |  Kaye, DM., et al. 1999. Biochem Biophys Res Commun. 256: 398-403. PMID: 10079196
  2. Akt激活NOS3并分别恢复H2O2胁迫人心脏微血管内皮屏障的完整性。  |  Dossumbekova, A., et al. 2008. Am J Physiol Heart Circ Physiol. 295: H2417-26. PMID: 18931031
  3. Akt1介导厚升支中嘌呤受体依赖性NOS3的激活。  |  Silva, GB. and Garvin, JL. 2009. Am J Physiol Renal Physiol. 297: F646-52. PMID: 19570880
  4. 血管紧张素II通过AT（2）受体和Akt1依赖性一氧化氮合成酶3（NOS3）激活刺激厚升支NO的产生。  |  Herrera, M. and Garvin, JL. 2010. J Biol Chem. 285: 14932-14940. PMID: 20299462
  5. 小鼠心脏骤停后，NOS3的基因缺失会增加致命性心脏功能障碍。  |  Beiser, DG., et al. 2011. Resuscitation. 82: 115-21. PMID: 20951489
  6. 磷酸二酯酶5A（PDE5A）定位于内皮细胞囊泡，可调节NOS3活性。  |  Gebska, MA., et al. 2011. Cardiovasc Res. 90: 353-63. PMID: 21421555
  7. 对抗 Fas 的敏感性与 NOS-3 过表达的 HepG2 细胞中增加的 cathepsin D 活性和肾上腺素还原酶表达无关。  |  Linares, CI., et al. 2015. Biochim Biophys Acta. 1853: 1182-94. PMID: 25712867
  8. 血浆凝血素通过增强巨噬细胞NOS3的功能，提高肺部对肺炎的防御能力。  |  Yang, Z., et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L11-6. PMID: 25957291
  9. 大麻二酚通过增强人结肠癌细胞中NOS3和SOD2诱导的自噬克服奥沙利铂耐药性。  |  Jeong, S., et al. 2019. Cancers (Basel). 11: PMID: 31195721
  10. MAP激酶通过两个独特的NOS3位点与NOS3结合并使其磷酸化。  |  Solone, XKV., et al. 2022. FEBS Open Bio. 12: 1075-1086. PMID: 35182051

订购信息

产品名称产品编号规格价格数量收藏夹

p-eNOS 抗体 (15E2)

sc-81510
50 µg/0.5 ml
CNY2422.00

p-eNOS (15E2): m-IgG Fc BP-HRP 套装

sc-539620
50 µg Ab; 10 µg BP
CNY2715.00

p-eNOS (15E2): m-IgG1 BP-HRP 套装

sc-541605
50 µg Ab; 20 µg BP
CNY2715.00

what do you recommend as apositive control in WB for this antibody?

Asked by: Doaa Babaeer
Thank you for your question. Here is a recommended positive control : https://www.scbt.com/p/huv-ec-c-whole-cell-lysate.
Answered by: BlakeJ
Date published: 2024-06-06

I am using p-NOS3 (15E2): sc-81510 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by PD2020 from It works in WBI tried this antibody in HUVEC cells and it worked in at 1:300 dilution
Date published: 2023-03-08
Rated 4 out of 5 by silg from Funziona bene nel mio sistemaHo provato l'anticorpo su cellule endoteliali primarie di arteria polmonare con la diluizione indicata in datasheet ed ha funzionato
Date published: 2020-11-24
Rated 5 out of 5 by Gross70 from phospho eNOS in keratinocytesGood p-eNOS signal in keratinocytes by WB and fibroblasts
Date published: 2018-11-16
Rated 4 out of 5 by 雨过天晴博 from weak bandI use 1:200 in WB,the band is very weak,the antibody for p-nos3 is difficult to use of various of bands of antibody such as CST and abcam,so I forgive it.
Date published: 2018-03-09
Rated 4 out of 5 by Ben Horst from Quality band in endothelial cellsGot a good band in HMvECs for Western blot. Would recommend
Date published: 2018-02-19
Rated 4 out of 5 by 小新贝贝 from A little weak1:500 diluted with 1 ％BSA for wb of rat protein,the band is little weak.
Date published: 2017-07-02
Rated 4 out of 5 by NZ from Good specificity and signal strength inGood specificity and signal strength in untreated (A) and pervanadate-treated (B) bend 3 whole cell lysates. -SCBT QC
Date published: 2013-06-11
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p-eNOS 抗体 (15E2) is rated 4.0 out of 5 by 7.
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