Date published: 2025-9-8

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p-eNOS 抗体 (15E2): sc-81510

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说明书
  • p-eNOS 抗体 15E2 是小鼠单克隆 IgG1, 在13篇文献中引用,提供 50 µg/0.5 ml
  • 与humanPARP-1的NOS3中丝氨酸1177周围的氨基酸残基对应的合成磷酸化肽
  • 推荐用于 mouse, rat 和 human 来源的Ser 1177 phosphorylated NOS3 WB 和 IP检测
  • 关于如何获取p-eNOS (15E2): sc-81510免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是p-eNOS Antibody (15E2) for WB applications. 的首选辅助检测试剂,这些试剂现与p-eNOS Antibody (15E2) 打包提供(请参阅下面的订购信息)。

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関連項目

p-NOS3抗体(15E2)是一种小鼠单克隆IgG1抗体,可通过蛋白质印迹(WB)和免疫沉淀(IP)检测小鼠、大鼠和人类样本中Ser 1177磷酸化NOS3。抗磷酸化NOS3抗体(15E2)以非结合形式提供,为各种实验设置提供了灵活性。NOS3,也称为内皮一氧化氮合酶,通过合成一氧化氮(NO)在心血管健康中发挥关键作用。一氧化氮是一种重要的信号分子,可调节血管张力、血压和血管生成。NOS3的正常运作有助于维持心血管的稳态;失调会导致各种心血管疾病。NOS3通过丝氨酸1177的磷酸化激活,这一过程由丝氨酸/苏氨酸蛋白激酶Akt/PKB介导,而丝氨酸/苏氨酸蛋白激酶Akt/PKB是磷脂酰肌醇3-激酶(PI 3-kinase)的重要下游靶标。这种磷酸化反应对于内皮细胞响应血管内皮生长因子(VEGF)等生长因子而迁移至关重要,凸显了NOS3在血管生成和组织修复中的重要性。p-NOS3 (15E2) 单克隆抗体使研究人员能够有效地研究NOS3的激活状态及其在各种生理和病理过程中的意义。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

p-eNOS 抗体 (15E2)

sc-81510
50 µg/0.5 ml
RMB2377.00

p-eNOS (15E2): m-IgG Fc BP-HRP 套装

sc-539620
50 µg Ab; 10 µg BP
RMB2662.00

p-eNOS (15E2): m-IgG1 BP-HRP 套装

sc-541605
50 µg Ab; 20 µg BP
RMB2662.00

what do you recommend as apositive control in WB for this antibody?

Asked by: Doaa Babaeer
Thank you for your question. Here is a recommended positive control : https://www.scbt.com/p/huv-ec-c-whole-cell-lysate.
Answered by: BlakeJ
Date published: 2024-06-06

I am using p-NOS3 (15E2): sc-81510 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from It works in WBI tried this antibody in HUVEC cells and it worked in at 1:300 dilution
Date published: 2023-03-08
Rated 4 out of 5 by from Funziona bene nel mio sistemaHo provato l'anticorpo su cellule endoteliali primarie di arteria polmonare con la diluizione indicata in datasheet ed ha funzionato
Date published: 2020-11-24
Rated 5 out of 5 by from phospho eNOS in keratinocytesGood p-eNOS signal in keratinocytes by WB and fibroblasts
Date published: 2018-11-16
Rated 4 out of 5 by from weak bandI use 1:200 in WB,the band is very weak,the antibody for p-nos3 is difficult to use of various of bands of antibody such as CST and abcam,so I forgive it.
Date published: 2018-03-09
Rated 4 out of 5 by from Quality band in endothelial cellsGot a good band in HMvECs for Western blot. Would recommend
Date published: 2018-02-19
Rated 4 out of 5 by from A little weak1:500 diluted with 1 %BSA for wb of rat protein,the band is little weak.
Date published: 2017-07-02
Rated 4 out of 5 by from Good specificity and signal strength inGood specificity and signal strength in untreated (A) and pervanadate-treated (B) bend 3 whole cell lysates. -SCBT QC
Date published: 2013-06-11
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p-eNOS 抗体 (15E2) is rated 4.0 out of 5 by 7.
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