Date published: 2025-9-6
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p-ERK 5 Antibody (1.T218/Y220) - Western Blotting - Image 93565
未经处理 (A, D), 经 EGF 处理 (B, E) 以及经 EGF 和 lambda 蛋白磷酸酶处理 (C, F) 的 HeLa 全细胞裂解液中 ERK 5 磷酸化的 Western 印迹分析. 测试的抗体包括 p-ERK 5 抗体 (1. T218/Y220): sc-135760 (A, B, C) 和 ERK 5 (C-20)-R: sc-1284-R (D, E, F).
p-ERK 5 Antibody (1.T218/Y220) - Western Blotting - Image 93565
未经处理 (A, D), 经 EGF 处理 (B, E) 以及经 EGF 和 lambda 蛋白磷酸酶处理 (C, F) 的 HeLa 全细胞裂解液中 ERK 5 磷酸化的 Western 印迹分析. 测试的抗体包括 p-ERK 5 抗体 (1. T218/Y220): sc-135760 (A, B, C) 和 ERK 5 (C-20)-R: sc-1284-R (D, E, F).
未经处理 (A, D), 经 EGF 处理 (B, E) 以及经 EGF 和 lambda 蛋白磷酸酶处理 (C, F) 的 HeLa 全细胞裂解液中 ERK 5 磷酸化的 Western 印迹分析. 测试的抗体包括 p-ERK 5 抗体 (1. T218/Y220): sc-135760 (A, B, C) 和 ERK 5 (C-20)-R: sc-1284-R (D, E, F).

p-ERK 5 抗体 (1.T218/Y220): sc-135760

4.0(1)
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说明书
  • p-ERK 5抗体(1.T218/Y220)是小鼠单克隆IgG1 κ, 在12篇文献中引用，规格为200 µg/ml
  • 针对ERK 5的human起源的双磷酸化的Thr 218和Tyr 220的短氨基酸序列
  • 抗-p-ERK 5 抗体 (1.T218/Y220) 推荐用于 WB, IP 和 ELISA，检测mouse, rat 和human 来源的 Thr 218 and Tyr 220 dually phosphorylated ERK 5
  • 抗p-ERK 5抗体（1.T218/Y220）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-ERK 5 (1.T218/Y220): sc-135760的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是p-ERK 5 Antibody (1.T218/Y220) for WB applications. 的首选辅助检测试剂，这些试剂现与p-ERK 5 Antibody (1.T218/Y220) 打包提供（请参阅下面的订购信息）。

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関連項目

附加ERK抗体，包括ERK 1, ERK 2, ERK 3和ERK 5

p-ERK 5抗体（1.T218/Y220）是一种IgG1κ小鼠单克隆p-ERK 5抗体，可通过WB、IP和ELISA检测小鼠、大鼠和人源性的Thr 218和Tyr 220双磷酸化ERK 5。p-ERK 5抗体（1.T218/Y220）有非偶联抗p-ERK 5抗体形式，以及多种偶联形式的抗p-ERK 5抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。生长因子、激素和神经递质对信号转导通路的激活是通过两种密切相关的MAP激酶p44和p42介导的，它们分别被指定为细胞外信号相关激酶1（ERK 1）和ERK 2。ERK蛋白通过在特征性Thr-Glu-Tyr模序内映射的特定酪氨酸和苏氨酸位点上的双磷酸化来调节。MAP激酶需要在苏氨酸218和酪氨酸220残基上进行双磷酸化才能获得酶活性。作为激活的响应，MAP激酶在丝氨酸和苏氨酸上磷酸化下游组分。MAP激酶上游调节因子包括MAP激酶激酶(MEK)、MEK激酶和Raf-1。ERK家族还有另外三个成员：ERK 3、ERK 5和ERK 6。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-ERK 5 抗体 (1.T218/Y220) 参考文献:

  1. ERK1和ERK2是两种微管相关蛋白2激酶，它们在原位介导酪氨酸羟化酶丝氨酸-31的磷酸化。  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
  2. 纯化磷酸化并激活 Erk-1 基因产物的鼠蛋白酪氨酸/苏氨酸激酶：与裂殖酵母 byr1 基因产物的关系。  |  Crews, CM. and Erikson, RL. 1992. Proc Natl Acad Sci U S A. 89: 8205-9. PMID: 1381507
  3. MEK 的主要结构，MEK 是一种蛋白激酶，能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  4. 光诱导小鼠胚胎干细胞分化与ERK5的激活有关。  |  Zou, GM., et al. 2006. Oncogene. 25: 463-9. PMID: 16247486
  5. 细胞外信号调节激酶 1（一种胰岛素刺激的微管相关蛋白 2 激酶）的纯化和特性。  |  Boulton, TG., et al. 1991. Biochemistry. 30: 278-86. PMID: 1846291
  6. 确定 pp42/丝裂原活化蛋白激酶（MAP 激酶）的调节磷酸化位点。  |  Payne, DM., et al. 1991. EMBO J. 10: 885-92. PMID: 1849075
  7. MEK5 和 ERK5 是 IGF-2 促进成肌作用的介质。  |  Carter, EJ., et al. 2009. J Cell Sci. 122: 3104-12. PMID: 19654213
  8. 在大鼠手术脑损伤模型中，神经营养素-3通过TrkC受体依赖性pErk5激活提供神经保护。  |  Akyol, O., et al. 2018. Exp Neurol. 307: 82-89. PMID: 29883578
  9. 富脯氨酸延展素样受体激酶 PERK5 和 PERK12 参与花粉管的生长。  |  Borassi, C., et al. 2021. FEBS Lett. 595: 2593-2607. PMID: 34427925
  10. 一种新的人类蛋白激酶信号转导途径的组成部分。  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
  11. 参与 C2C12 肌母细胞分化的有丝分裂原激活蛋白激酶 ERK6  |  Lechner, C., et al. 1996. Proc Natl Acad Sci U S A. 93: 4355-9. PMID: 8633070

订购信息

产品名称产品编号规格价格数量收藏夹

p-ERK 5 抗体 (1.T218/Y220)

sc-135760
200 µg/ml
RMB2377.00

p-ERK 5 (1.T218/Y220): m-IgG Fc BP-HRP 套装

sc-527101
200 µg Ab; 10 µg BP
RMB2662.00

p-ERK 5 (1.T218/Y220): m-IgG1 BP-HRP 套装

sc-532474
200 µg Ab; 20 µg BP
RMB2662.00

p-ERK 5 抗体 (1.T218/Y220) AC

sc-135760 AC
500 µg/ml, 25% agarose
RMB3129.00

p-ERK 5 抗体 (1.T218/Y220) HRP

sc-135760 HRP
200 µg/ml
RMB2377.00

p-ERK 5 抗体 (1.T218/Y220) FITC

sc-135760 FITC
200 µg/ml
RMB2482.00

p-ERK 5 抗体 (1.T218/Y220) PE

sc-135760 PE
200 µg/ml
RMB2580.00

p-ERK 5 抗体 (1.T218/Y220) Alexa Fluor® 488

sc-135760 AF488
200 µg/ml
RMB2685.00

p-ERK 5 抗体 (1.T218/Y220) Alexa Fluor® 546

sc-135760 AF546
200 µg/ml
RMB2685.00

p-ERK 5 抗体 (1.T218/Y220) Alexa Fluor® 594

sc-135760 AF594
200 µg/ml
RMB2685.00

p-ERK 5 抗体 (1.T218/Y220) Alexa Fluor® 647

sc-135760 AF647
200 µg/ml
RMB2685.00

p-ERK 5 抗体 (1.T218/Y220) Alexa Fluor® 680

sc-135760 AF680
200 µg/ml
RMB2685.00

p-ERK 5 抗体 (1.T218/Y220) Alexa Fluor® 790

sc-135760 AF790
200 µg/ml
RMB2685.00

I'm trying to get pERK5 western blot band but I get too many non-specific bands that I cannot even distinguish which is pERK5. I'm using 5% BSA solution for blocking. (Tried 1h, 2h and 24h blocking but all have non specific bands). What's the problem..?

Asked by: Hannah H
Thank you for your product question. We are sorry to hear that this product did not perform as expected. We want you to be successful in your research, please contact our Technical Service Department for support by phone, e-mail or live chat.
Answered by: Technical Service SH
Date published: 2023-01-09

Dear Sir/Madam, I bought this pERK5 antibody a month ago and tried it in rat aortic endothelial cells. The western blot gave me no bands between 100 and 150 kDA. The dilution i tried was 1:250, will you please advise me further troubleshooting tips.

Asked by: Anonymous
Thank you for your question. Please contact Technical Service directly for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2021-02-09

What was the concentration and duration EGF treatment for Hela's as a positive control for this antibody?

Asked by: akuraki
Thank you for your question. We used EGF at a concentration of 5ng/ml for 4 hours at 37ºC for this induction.
Answered by: Technical Support
Date published: 2017-07-18

Are there any issues with using sc-135760: p-ERK 5 (1.T218/Y220) mouse monoclonal primary antibody on mouse samples?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-135760 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from Good Western blot data in analyzing ERK 5Good Western blot data in analyzing ERK 5 phosphorylation in EGF treated and EGF and lambda protein phosphatase treated HeLa whole cell lysates. Some bacckground bands detected -SCBT QC
Date published: 2013-05-02
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p-ERK 5 抗体 (1.T218/Y220) is rated 4.0 out of 5 by 1.
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