Date published: 2025-9-7

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p-Cofilin 1 抗体 (F-11): sc-365882

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说明书
  • p-Cofilin 1抗体(F-11)是小鼠单克隆IgG2b κ, 在4篇文献中引用,规格为200 µg/ml
  • 抗-p-Cofilin 1 抗体 (F-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Ser 3 phosphorylated Cofilin 1
  • 抗p-Cofilin 1抗体(F-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-Cofilin 1 (F-11): sc-365882免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2b BP-HRP 是 p-Cofilin 1 抗体 (F-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-Cofilin 1 抗体 (F-11) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

p-Cofilin 1抗体(F-11)是一种IgG2b κ小鼠单克隆p-Cofilin 1抗体(也称为p-Cofilin 1抗体或Cofilin抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的Ser 3磷酸化Cofilin 1。p-Cofilin 1抗体(F-11)以非偶联的抗p-Cofilin 1抗体形式提供,同时也提供多种偶联形式的抗p-Cofilin 1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。LIM激酶1(LIMK-1)是一种含有LIM和PDZ结构域的丝氨酸/苏氨酸激酶。LIMK1在体内外均可在丝氨酸3处使cofilin磷酸化。Cofilin是一种肌动蛋白解聚因子,调节肌动蛋白细胞骨架的重组。已知在丝氨酸3上的cofilin磷酸化会阻断这些活动。LIMK1或其他酶对ADF/cofilin蛋白的磷酸化将通过抑制这些蛋白水解其结合的ATP并解离磷酸盐来切断和解聚较老的肌动蛋白丝的能力,从而趋于稳定肌动蛋白丝。肌动蛋白丝的快速周转和三级网状结构的形成受多种肌动蛋白结合蛋白的调节。因此,Cofilin是引起肌动蛋白细胞骨架重排的信号级联的终端效应器。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p-Cofilin 1 抗体 (F-11)

sc-365882
200 µg/ml
RMB2377.00

p-Cofilin 1 (F-11): m-IgG Fc BP-HRP 套装

sc-525905
200 µg Ab; 10 µg BP
RMB2662.00

p-Cofilin 1 (F-11): m-IgG2b BP-HRP 套装

sc-549646
200 µg Ab; 10 µg BP
RMB2662.00

p-Cofilin 1 抗体 (F-11) AC

sc-365882 AC
500 µg/ml, 25% agarose
RMB3129.00

p-Cofilin 1 抗体 (F-11) HRP

sc-365882 HRP
200 µg/ml
RMB2377.00

p-Cofilin 1 抗体 (F-11) FITC

sc-365882 FITC
200 µg/ml
RMB2482.00

p-Cofilin 1 抗体 (F-11) PE

sc-365882 PE
200 µg/ml
RMB2580.00

p-Cofilin 1 抗体 (F-11) Alexa Fluor® 488

sc-365882 AF488
200 µg/ml
RMB2685.00

p-Cofilin 1 抗体 (F-11) Alexa Fluor® 546

sc-365882 AF546
200 µg/ml
RMB2685.00

p-Cofilin 1 抗体 (F-11) Alexa Fluor® 594

sc-365882 AF594
200 µg/ml
RMB2685.00

p-Cofilin 1 抗体 (F-11) Alexa Fluor® 647

sc-365882 AF647
200 µg/ml
RMB2685.00

p-Cofilin 1 抗体 (F-11) Alexa Fluor® 680

sc-365882 AF680
200 µg/ml
RMB2685.00

p-Cofilin 1 抗体 (F-11) Alexa Fluor® 790

sc-365882 AF790
200 µg/ml
RMB2685.00

p-Cofilin 1 (F-11) 中和勝肽

sc-365882 P
100 µg/0.5 ml
RMB511.00

I purchased Anti-p-Cofilin 1 Antibody (F-11): sc-365882. I would like to know, it is raised in which animal? The datasheet says, it is anti-mouse but anti-mouse is not working for us rather anti-rabbit is giving a signal close to the true signal.

Asked by: SwetaK
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-01-07

What application is the blocking peptide sc-365882 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2023-01-07
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Rated 4 out of 5 by from Greatfirst antibody from SCT that I felt deserved a review because it worked great!
Date published: 2018-10-19
Rated 5 out of 5 by from Great AbUsed a 1:300 dilution for WB with 1- SUM159, 2- Caco-2, and 3- 293-T cell lysates. Detected a very clean and strong band.
Date published: 2018-01-30
Rated 5 out of 5 by from very goodit is easy to detect p-cofilin1 band. Good antibody.
Date published: 2017-06-27
Rated 5 out of 5 by from definitely phospho specific for CFL1 in WBTested in western blots at 1000X dilution and produced a very specific and strong western signal.
Date published: 2017-03-20
Rated 5 out of 5 by from Nice pCofflin1 detection in JurkatWe used this antibody for expression detection of pCofflin1 by western blot. We got a very nice band around the correct molecular weight in Jurkat cells.
Date published: 2017-02-08
Rated 5 out of 5 by from Very potent and specific antibodyThis monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is highly recommended for Western blotting. There was strong detection of the ~15 kDa cofilin target in SDS-PAGE solubilized lysates from overnight serum-starved human A431 cells treated for 5 minutes with EGF on Western blots, and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2016-12-04
Rated 5 out of 5 by from Nice Western Blot data of Cofilin 1 phosphorylationNice Western Blot data of Cofilin 1 phosphorylation in untreated and lambda protein phosphatase treated NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2014-07-22
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p-Cofilin 1 抗体 (F-11) is rated 4.9 out of 5 by 7.
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