Date published: 2025-9-7

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p-Cofilin 1 抗体 (E-5): sc-271921

5.0(11)
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说明书
  • p-Cofilin 1抗体(E-5)是小鼠单克隆IgM κ, 在13篇文献中引用,规格为200 µg/ml
  • 与含磷酸化Ser 3的短氨基酸序列杂交,该序列来自Cofilin 1的human起源
  • 推荐用于 mouse, rat 和 human 来源的Ser 3 phosphorylated Cofilin 1 WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应,包括: 和 bovine and porcine
  • 关于如何获取p-Cofilin 1 (E-5): sc-271921免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是p-Cofilin 1 Antibody (E-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与p-Cofilin 1 Antibody (E-5) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

p-Cofilin 1抗体(E-5)是一种IgM κ小鼠单克隆p-Cofilin 1抗体(也称为p-Cofilin 1抗体或Cofilin抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的Ser 3磷酸化Cofilin 1。p-Cofilin 1抗体(E-5)以非偶联的抗p-Cofilin 1抗体形式提供。LIM激酶1(LIMK-1)是一种含有LIM和PDZ结构域的丝氨酸/苏氨酸激酶。LIMK1在体内外均可在丝氨酸3处使cofilin磷酸化。Cofilin是一种肌动蛋白解聚因子,调节肌动蛋白细胞骨架的重组。已知在丝氨酸3上的cofilin磷酸化会阻断这些活动。LIMK1或其他酶对ADF/cofilin蛋白的磷酸化将通过抑制这些蛋白水解其结合的ATP并解离磷酸盐来切断和解聚较老的肌动蛋白丝的能力,从而趋于稳定肌动蛋白丝。肌动蛋白丝的快速周转和三级网状结构的形成受多种肌动蛋白结合蛋白的调节。因此,Cofilin是引起肌动蛋白细胞骨架重排的信号级联的终端效应器。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

p-Cofilin 1 抗体 (E-5)

sc-271921
200 µg/ml
RMB2377.00

p-Cofilin 1 (E-5): m-IgGκ BP-HRP 套装

sc-551996
200 µg Ab; 40 µg BP
RMB2662.00

p-Cofilin 1 (E-5) 中和勝肽

sc-271921 P
100 µg/0.5 ml
RMB511.00

Has this antibody been tested on frozen tissue sections for immunofluorescence, specifically mouse tissues? Are any antigen retrieval steps recommended?

Asked by: HB23
Thank you for your question. sc-271921 is recommended for mouse, rat, human , bovine , and porcine. We validate this antibody for WB, IP, IF, IHC(P) and ELISA. Please see attached protocol for antigen retrieval. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2023-03-16

Does this antibody (or any of the other anti-cofilin antibodies currently available) recognize Drosophila Cofilin by IF?

Asked by: JSR1
Thank you for your question. Sequence analysis shows very low to no homology between the epitope sequence of the antibodies and the target in Drosophila. If you have additional questions, please contact Technical Support at 1800-457-3801 option 2, by emailing [email protected] or by live chat on our website at www.scbt.com so we may further assist you.
Answered by: Santa Cruz TS
Date published: 2021-07-07

What application is the blocking peptide sc-271921 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Anti-p-Cofilin per analisi in western blotL'Ab è stato testato utilizzando lisati cellulari MCF-7 e MDA-MD-231, ottenuti in RIPA buffer. 15 ug di proteine sono state separate tramite SDS-Page e trasferite su un filtro di nitrocellulosa. Il bloccaggio è stato effettuato in Blotto A per 1h, l'Ab primario è stato utilizzato alla diluizione di 1:1000 in 10 mL di Blotto A per 1h. Il secondario 1:2000 in Blotto A per 1h. Il segnale ottenuto è pulito, con un'unica banda del perso molecolare atteso. La specificità del segnale è stata verificata utilizzando l'inibitore della chinasi ROCK, Y-27632.
Date published: 2024-07-23
Rated 5 out of 5 by from Great antibody for western blottingWe used 1:1000 on western blotting from NPCs, it give a nice and clear band.
Date published: 2018-07-19
Rated 5 out of 5 by from Great AbUsed a 1:300 dilution for WB with 1- SUM159, 2- Caco-2, and 3- 293-T cell lysates. detected a very clean and strong band.
Date published: 2018-01-30
Rated 5 out of 5 by from Works very well on WBI used this antibody on brain tissue (protein load: 30 uL, Ab concentration: 1/1000), and it worked very well: one band and good intensity for quantification.
Date published: 2018-01-08
Rated 5 out of 5 by from The band is present and abundantThis antibody worked great with HEK293 whole cell lysis at 1:500 dilution and incubation with 5% BSA
Date published: 2017-11-09
Rated 5 out of 5 by from good antibodyI used it at 1:150 dilution with milk and worked very well.
Date published: 2017-04-14
Rated 5 out of 5 by from Sensitive and SpecificGreat specificity to phosphorylated Cofilin 1 as shown by phosphatase treatment.
Date published: 2017-01-16
Rated 5 out of 5 by from Very potent and highly specific antibodyThis monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and determined to be an excellent antibody for Western blotting. Strong detection of cofilin 1 was observed, but no cross-reactivities detected by Western blotting in HeLa cells exposed to anisomycin for 2 hours. No detection of the cofilin 1 target was observed in overnight-serum starved human A431 cells that were treated for 5 minutes with epidermal growth factor. However, there was no significant cross-reactivities on these A431 cell immunoblots with any off-target proteins.
Date published: 2016-12-08
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p-Cofilin 1 抗体 (E-5) is rated 5.0 out of 5 by 11.
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