Date published: 2025-9-7
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p-Akt1 Antibody (5.Ser 473) - Western Blotting - Image 159341
未经处理 (A, D), 钙culin A 处理 (B, E) 和钙culin A 及λ蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 Jurkat 全细胞裂解液中 Akt1 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Akt1 抗体 (5. Ser 473): sc-293125 (A, B, C) 和 Akt1 (C-20): sc-1618 (D, E, F).
p-Akt1 Antibody (5.Ser 473) - Western Blotting - Image 159341
未经处理 (A, D), 钙culin A 处理 (B, E) 和钙culin A 及λ蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 Jurkat 全细胞裂解液中 Akt1 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Akt1 抗体 (5. Ser 473): sc-293125 (A, B, C) 和 Akt1 (C-20): sc-1618 (D, E, F).
p-Akt1 Antibody (5.Ser 473) - Western Blotting - Image 158395
未经处理 (A, D), 经 mLIF 处理 (B, E) 以及经 mLIF 和 lambda 蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 3T3-L1 全细胞裂解液中 Akt1 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Akt1 抗体 (5. Ser 473): sc-293125 (A, B, C) 和 Akt1 (C-20): sc-1618 (D, E, F).
p-Akt1 Antibody (5.Ser 473) - Western Blotting - Image 394361
p-Akt1 抗体 (5. Ser 473) HRP: sc-293125 HRP. Jurkat (A) 和 Jurkat + PMA 诱导 (B) 全细胞裂解液中 Akt1 磷酸化的直接 Western 印迹分析.
未经处理 (A, D), 钙culin A 处理 (B, E) 和钙culin A 及λ蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 Jurkat 全细胞裂解液中 Akt1 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Akt1 抗体 (5. Ser 473): sc-293125 (A, B, C) 和 Akt1 (C-20): sc-1618 (D, E, F).

p-Akt1 抗体 (5.Ser 473): sc-293125

4.2(17)
寫評論提問

说明书
  • p-Akt1抗体(5.Ser 473)是小鼠单克隆IgG1 κ, 在129篇文献中引用，规格为200 µg/ml
  • 针对含磷酸化Ser 473的短氨基酸序列的抗体，该序列来自Akt1 of human
  • 推荐用于 mouse, rat 和 human 来源的Ser 473 phosphorylated Akt1 WB, IP 和 ELISA检测; 可能与 Ser 473 phosphorylated Akt2 and Ser 473 phosphorylated Akt3产生交联反应
  • 还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
  • 关于如何获取p-Akt1 (5.Ser 473): sc-293125的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是p-Akt1 Antibody (5.Ser 473) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p-Akt1 Antibody (5.Ser 473) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

附加Akt抗体，包括Akt1, Akt2和Akt3

p-Akt1抗体（5.Ser 473）是一种IgG1 κ小鼠单克隆p-Akt1抗体，可通过WB、IP和ELISA检测小鼠、大鼠和人类来源的Ser 473磷酸化Akt1。p-Akt1抗体（5.Ser 473）可作为偶联和非偶联的抗p-Akt1抗体形式提供。丝氨酸/苏氨酸激酶Akt家族包含几个成员，包括Akt1（也称为PKB或RacPK）、Akt2（也称为PKBβ或RacPK-β）和Akt 3（也称为PKBγ或thyoma病毒原发癌基因3），它们与蛋白激酶A和C家族具有序列同源性，并由c-Akt原癌基因编码。Akt家族的所有成员都具有血小板同源结构域。Akt1和Akt2通过PDGF刺激被激活。这种激活依赖于PDGFR-β酪氨酸残基740和751，它们与磷脂酰肌醇3-激酶（PI 3-激酶）复合物的亚基结合。胰岛素或胰岛素生长因子-1（IGF-1）对Akt1的激活导致Thr 308和Ser 473的磷酸化。在胰岛素/IGF-1刺激的细胞中，Akt蛋白通过上游激酶被磷酸化和激活，而Akt1和Akt2的激活被PI激酶抑制剂wortmannin抑制。综上所述，这些数据充分表明该蛋白是PI激酶下游的信号分子。Akt3在丝氨酸残基上被磷酸化以响应胰岛素。然而，胰岛素对Akt3的激活被蛋白激酶C的先前激活所抑制，这种机制不需要PH结构域的存在。Akt3在3T3-L1成纤维细胞、脂肪细胞和骨骼肌中表达，可能参与各种生物过程，包括脂肪细胞和肌肉分化、糖原合成、葡萄糖摄取、细胞凋亡和细胞增殖。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Akt1 抗体 (5.Ser 473) 参考文献:

  1. 鉴定含有丝氨酸磷酸化调节位点的人类 Akt3（蛋白激酶 B γ）。  |  Nakatani, K., et al. 1999. Biochem Biophys Res Commun. 257: 906-10. PMID: 10208883
  2. 通过荧光原位杂交将编码Akt/蛋白激酶B家族成员的AKT3绘制到人类和啮齿动物的染色体上。  |  Murthy, SS., et al. 2000. Cytogenet Cell Genet. 88: 38-40. PMID: 10773662
  3. 与胃癌细胞周期异常相关的新型 AKT1 突变。  |  Ghatak, S., et al. 2018. Per Med. 15: 79-86. PMID: 29714127
  4. 外周支气管乳头状瘤中的 AKT1 基因突变 腺乳头状瘤和鳞状细胞与腺乳头状瘤混合瘤与支气管腺瘤的区别。  |  Sasaki, E., et al. 2021. Am J Surg Pathol. 45: 119-126. PMID: 32868527
  5. 多发性硬化症患者外周T细胞亚群中Akt1和p-Akt1的表达。  |  Oktelik, FB., et al. 2021. Acta Neurol Belg. 121: 1777-1782. PMID: 33034831
  6. AKT1（p. E17K）热点突变对恶性肿瘤发生和预后的影响  |  Chen, Y., et al. 2020. Front Cell Dev Biol. 8: 573599. PMID: 33123537
  7. 磷脂酰肌醇-3-OH 激酶信号转导中的蛋白激酶 B（c-Akt）。  |  Burgering, BM. and Coffer, PJ. 1995. Nature. 376: 599-602. PMID: 7637810
  8. Akt 原癌基因编码的蛋白激酶是 PDGF 激活的磷脂酰肌醇 3- 激酶的靶标。  |  Franke, TF., et al. 1995. Cell. 81: 727-36. PMID: 7774014
  9. AH/PH 结构域介导的 Akt 分子间相互作用及其在 Akt 调节中的潜在作用。  |  Datta, K., et al. 1995. Mol Cell Biol. 15: 2304-10. PMID: 7891724
  10. 人胰腺细胞中 AKT2 的扩增以及反义 RNA 对 AKT2 表达和致瘤性的抑制。  |  Cheng, JQ., et al. 1996. Proc Natl Acad Sci U S A. 93: 3636-41. PMID: 8622988
  11. 蛋白激酶 C 可调节 3T3-L1 脂肪细胞在胰岛素刺激下增加的 Akt1 和 Akt3 活性。  |  Barthel, A., et al. 1998. Biochem Biophys Res Commun. 243: 509-13. PMID: 9480839
  12. 体内大鼠骨骼肌中的 Akt 激酶和 2-脱氧葡萄糖摄取：与胰岛素和运动有关的研究。  |  Turinsky, J. and Damrau-Abney, A. 1999. Am J Physiol. 276: R277-82. PMID: 9887206

订购信息

产品名称产品编号规格价格数量收藏夹

p-Akt1 抗体 (5.Ser 473)

sc-293125
200 µg/ml
$316.00

p-Akt1 (5.Ser 473): m-IgG Fc BP-HRP 套装

sc-529297
200 µg Ab; 10 µg BP
$354.00

p-Akt1 (5.Ser 473): m-IgGκ BP-HRP 套装

sc-522114
200 µg Ab, 40 µg BP
$354.00

p-Akt1 (5.Ser 473): m-IgG1 BP-HRP 套装

sc-543437
200 µg Ab; 20 µg BP
$354.00

p-Akt1 抗体 (5.Ser 473) AC

sc-293125 AC
500 µg/ml, 25% agarose
$416.00

p-Akt1 抗体 (5.Ser 473) HRP

sc-293125 HRP
200 µg/ml
$316.00

What is the exact MW of phospho Akt and phospho ERK? Sc 7985R and Sc 101 760 catalog number, respectively. Rabbit polyclonal

Asked by: Marialice
Thank you for your question. The expected molecular weight for these phosphorylated proteins is published on our antibody data sheets. You can still download the data sheets for these now discontinued antibodies by searching on our website with the Data Sheet search, or use the link below: https://www.scbt.com/resources/datasheet-search
Answered by: Technical Support
Date published: 2020-03-23

What secondary is best to use with p-Akt1 (5.Ser 473): sc-293125?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with p-Akt1 (5.Ser 473): sc-293125 monoclonal antibody, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-17
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Rated 5 out of 5 by from sc-293125 sample pAkt western blotI received this antibody as sample and I use it in a western blot experiment of proteins obtained from mouse neural stem cells. I found the result excellent even without any setup, just following the data sheet indication. I got a single band of the correct size. I think I will buy the full size antibody
Date published: 2025-05-08
Rated 1 out of 5 by from Non funziona su estratti proteici di topoHo provato la trial size circa un mese fa. Purtroppo non funziona per western blot su estratti proteici preparati da muscolo di topo. Riconosce soltanto le catene leggere e pesanti delle immunoglobuline a 20 kDae 55kDa, rispettivamente
Date published: 2023-04-14
Rated 5 out of 5 by from Very good resultsWorked good on tumor epithelial cells, IHC staining, 1:100 dilution.
Date published: 2018-07-24
Rated 5 out of 5 by from This antibody works for western blot.We acquired this antibody about 7 months ago. We performed western blot using HeLa cells whole cell lysates at a dilution of 1 to 1000. We were able to detect the protein with one minute exposure. This was repeated twice with success.
Date published: 2018-06-14
Rated 4 out of 5 by from Good antibodiesI used this antibodies in Western blot in dilution 1:1000. It works good but in little background.
Date published: 2018-06-10
Rated 5 out of 5 by from Excellent antibodyThis antibody works very well with minimal background.
Date published: 2018-05-02
Rated 3 out of 5 by from p-AKT1 (5.Ser 473)I used this antibodies at 1:1000 dilution on western blot with 30ug of protein from cell line.After immunodetection was a lot of non-specific bands higher and lower from the correct band, so it was difficult to discern correct bands.
Date published: 2018-04-06
Rated 3 out of 5 by from Good for Akt2, but poor for Akt1 or Akt3I used this antibody at 1:1000 dilution on a western blot with 100 ug of protein from tissue. The 55 kDa band was great for Akt2, but very weak for Akt1 or Akt3. Also, many non-specific bands both higher and lower from the correct kDa sizes making it slightly difficult to discern correct bands to analyze.
Date published: 2018-02-21
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p-Akt1 抗体 (5.Ser 473) is rated 4.2 out of 5 by 17.
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