Date published: 2025-9-7
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Akt1 Antibody (B-1) - Western Blotting - Image 392794
Akt1 抗体 (B-1) Alexa Fluor® 488: sc-5298 AF488. IMR-32 (A), NIH/3T3 (B), A549 (C), T-47D (D) 和 MCF7 (E) 全细胞裂解液中 Akt1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Akt1 抗体 (B-1) Alexa Fluor® 488: sc-5298 AF488. IMR-32 (A), NIH/3T3 (B), A549 (C), T-47D (D) 和 MCF7 (E) 全细胞裂解液中 Akt1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Akt1 抗体 (B-1): sc-5298

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说明书
  • Akt1 抗体 B-1 是小鼠单克隆 IgG1 κ，Akt1抗体, 在728篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Akt1的氨基酸345-480
  • Akt1 抗体 (B-1) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 Akt1
  • 抗Akt1抗体（B-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Akt1 (B-1): sc-5298的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Akt1 抗体 (B-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Akt1 抗体 (B-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Akt 抗体 包括 Akt1, Akt2和Akt3

Akt1抗体（B-1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过WB、IP、IF、IHCP、FCM和ELISA检测小鼠、大鼠和人源的Akt1。Akt1单克隆抗体（B-1）有非结合型和多种结合型，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合物。Akt1，也称为蛋白激酶B（PKB），在细胞信号传导通路中起着至关重要的作用，可调节细胞生长、存活和新陈代谢。Akt1主要位于细胞质中，在胰岛素和血小板衍生生长因子（PDGF）等生长因子激活后，可转移到细胞膜上。这种转移对于激活至关重要，因为Akt1与磷脂酰肌醇依赖性激酶-1（PDK1）相互作用，后者在Thr 308处对Akt1进行磷酸化，这是完全激活的关键步骤。此外，Akt1对各种下游靶标进行磷酸化，对于调节细胞对生长信号的反应至关重要，这使得Akt1在葡萄糖代谢和细胞凋亡等过程中扮演着关键角色。Akt1在Ser 473位点的磷酸化虽然不是Thr 308位点磷酸化所必需的，但可以进一步增强活性，表明可以促进细胞存活和生长。Akt1的调节过程非常复杂，涉及各种上游信号通路，在正常生理和疾病状态（包括癌症）中均具有重要意义。这些特性使Akt1单克隆抗体（B-1）成为研究Akt1介导的信号通路及其在细胞过程和疾病研究中的作用的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Akt1 抗体 (B-1) 参考文献:

  1. 鉴定含有丝氨酸磷酸化调节位点的人类 Akt3（蛋白激酶 B γ）。  |  Nakatani, K., et al. 1999. Biochem Biophys Res Commun. 257: 906-10. PMID: 10208883
  2. 通过荧光原位杂交将编码Akt/蛋白激酶B家族成员的AKT3绘制到人类和啮齿动物的染色体上。  |  Murthy, SS., et al. 2000. Cytogenet Cell Genet. 88: 38-40. PMID: 10773662
  3. AKT1 为原代人类角质形成细胞的分化和分层提供了必要的生存信号。  |  Thrash, BR., et al. 2006. J Biol Chem. 281: 12155-62. PMID: 16517604
  4. AKT1/BRCA1在控制同源重组和遗传稳定性中的作用：遗传性和散发性乳腺癌之间的缺失环节。  |  Guirouilh-Barbat, J., et al. 2010. Oncotarget. 1: 691-699. PMID: 21321378
  5. AKT1介导多种磷酸化作用，从而促进HSF1的激活。  |  Lu, WC., et al. 2022. FEBS J. 289: 3876-3893. PMID: 35080342
  6. 向人类细胞输送活性 AKT1。  |  Siddika, T., et al. 2022. Cells. 11: PMID: 36497091
  7. 磷脂酰肌醇-3-OH 激酶信号转导中的蛋白激酶 B（c-Akt）。  |  Burgering, BM. and Coffer, PJ. 1995. Nature. 376: 599-602. PMID: 7637810
  8. Akt 原癌基因编码的蛋白激酶是 PDGF 激活的磷脂酰肌醇 3- 激酶的靶标。  |  Franke, TF., et al. 1995. Cell. 81: 727-36. PMID: 7774014
  9. AH/PH 结构域介导的 Akt 分子间相互作用及其在 Akt 调节中的潜在作用。  |  Datta, K., et al. 1995. Mol Cell Biol. 15: 2304-10. PMID: 7891724
  10. 人胰腺细胞中 AKT2 的扩增以及反义 RNA 对 AKT2 表达和致瘤性的抑制。  |  Cheng, JQ., et al. 1996. Proc Natl Acad Sci U S A. 93: 3636-41. PMID: 8622988
  11. 蛋白激酶 C 可调节 3T3-L1 脂肪细胞在胰岛素刺激下增加的 Akt1 和 Akt3 活性。  |  Barthel, A., et al. 1998. Biochem Biophys Res Commun. 243: 509-13. PMID: 9480839
  12. 体内大鼠骨骼肌中的 Akt 激酶和 2-脱氧葡萄糖摄取：与胰岛素和运动有关的研究。  |  Turinsky, J. and Damrau-Abney, A. 1999. Am J Physiol. 276: R277-82. PMID: 9887206

订购信息

产品名称产品编号规格价格数量收藏夹

Akt1 抗体 (B-1)

sc-5298
200 µg/ml
RMB2377.00

Akt1 (B-1): m-IgG Fc BP-HRP 套装

sc-528153
200 µg Ab; 10 µg BP
RMB2662.00

Akt1 (B-1): m-IgGκ BP-HRP 套装

sc-520461
200 µg Ab, 40 µg BP
RMB2662.00

Akt1 (B-1): m-IgG1 BP-HRP 套装

sc-542789
200 µg Ab; 20 µg BP
RMB2662.00

Akt1 抗体 (B-1) AC

sc-5298 AC
500 µg/ml, 25% agarose
RMB3129.00

Akt1 抗体 (B-1) HRP

sc-5298 HRP
200 µg/ml
RMB2377.00

Akt1 抗体 (B-1) FITC

sc-5298 FITC
200 µg/ml
RMB2482.00

Akt1 抗体 (B-1) PE

sc-5298 PE
200 µg/ml
RMB2580.00

Akt1 抗体 (B-1) Alexa Fluor® 488

sc-5298 AF488
200 µg/ml
RMB2685.00

Akt1 抗体 (B-1) Alexa Fluor® 546

sc-5298 AF546
200 µg/ml
RMB2685.00

Akt1 抗体 (B-1) Alexa Fluor® 594

sc-5298 AF594
200 µg/ml
RMB2685.00

Akt1 抗体 (B-1) Alexa Fluor® 647

sc-5298 AF647
200 µg/ml
RMB2685.00

Akt1 抗体 (B-1) Alexa Fluor® 680

sc-5298 AF680
200 µg/ml
RMB2685.00

Akt1 抗体 (B-1) Alexa Fluor® 790

sc-5298 AF790
200 µg/ml
RMB2685.00

I recently used your antibody. I noticed that it gives me signal at two different molecular weight heights: one band is at 60 kDa while the other band is around 100 kDa. How is it possible? How can i explain it?

Asked by: APq_
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-03-01

hello. My name is Ridvan. I am an researcher assistant in Eskisehir Osmangazi University in Eskisehir/Turkey. Can you send me Immunohistochemistry ve Western Blot protocols of Akt-1 (Sc-5298) and p-Akt 1/2/3 antibodies (sc-514032 ). Thank You very much.

Asked by: rido
Thank you for your question. Our recommended protocols for use with both antibodies can be found online. Please contact us for further Technical Support in using these antibodies.
Answered by: Technical Support
Date published: 2019-05-03

is there reactivity for rabbit?

Asked by: exertion7
Thank you for your question. This antibody has been published over 400 times, and we are not aware of any publications in rabbit. Please contact our Technical Service Department if you would like assistance performing sequence analysis using the antibody’s antigen to predict cross reactivity with rabbit.
Answered by: Technical Support
Date published: 2019-04-13

Hi, Where I can check what materials used to wash nitrocellulose or PVDF membrane?

Asked by: Robert1124
Thank you for your question. We recommend washing the membrane with TBS and TBST. The details can be found in our protocol online.
Answered by: Technical Support
Date published: 2019-02-23

Among the different indications of this antibody: Akt1 (B-1): sc-5298, it is its ability to recognize the phosphorylated-AKT1. Since there are different phosphorylation sites, I need to be sure that it applies also for the S473-phosphorylated AKT1.

Asked by: stefania70
Thank you for your question. This antibody can detect the Akt1 protein, both phosphorylated and non-phosphorylated. It is not phospho-specific and does therefore not specifically detect a certain phosphorylation site. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2018-11-30

Dear Whome it may concern, We have some antibodies from other resources that are not work well, and we would like to try a few antibodies. How can we receive free samples? We can send pictures of our not working antibodies if needed.

Asked by: Sevilay Acar
Thank you you for your request. Please contact our European Technical Service team by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com, we would be happy to send you any samples you would like to try.
Answered by: Tech Service
Date published: 2017-10-10

What is the recommended secondary reagent to be used with the Akt1 Antibody (B-1) for Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-22

Akt1 Antibody (B-1): sc-5298 1) Does it only binds Akt1, or also binds phosphorylated Akt1? 2) How to get a 10 ug sample? How much do we need to pay?

Asked by: tua64317
Thank you for your question. This monoclonal, Akt1 Antibody (B-1): sc-5298, is able to recognize Akt1 and phosphorylated Akt1. This is demonstrated in the Western blot data on our website showing evaluation of inactive and active human recombinant Akt1. Please contact our Technical Service Department to request a complimentary 10 µg sample of this mouse monoclonal antibody for testing. The sample is free of charge. Please call us directly at (800) 457-3801 option 2, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2016-12-21
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Rated 5 out of 5 by from sc-5298-sampleI used this sample in Western blot using my mouse NSCs. I found the result excellent even without any setup, just following the data sheet indication. I got a single band of the correct size.
Date published: 2025-05-08
Rated 4 out of 5 by from WonderfulQuite Good Antibody,worked well in WB(specilfic bands)
Date published: 2024-05-09
Rated 5 out of 5 by from Buena señalProbamos anti AKT1 y obtuvimos buena señal. Adjunto foto
Date published: 2024-03-14
Rated 5 out of 5 by from Great antibodyIused this antibody (1:1000) and it worked fine for my purposes
Date published: 2023-07-18
Rated 5 out of 5 by from Great antibodyIused this antibody (1:1000) and it worked fine for my purposes
Date published: 2023-07-18
Rated 5 out of 5 by from Work nicely in WBAntibody sc-5298 (1:1000) worked pretty well in MDA-MB-231 cell lysates
Date published: 2023-07-11
Rated 4 out of 5 by from Buon funzionamentoHo testato il campione omaggio di questo anticorpo e ho avuto buoni risultati
Date published: 2023-04-18
Rated 5 out of 5 by from anti-Akt1 (B-1) antibodyThe anti-Akt1 (B-1) monoclonal antibody worked very well in our Western blotting.
Date published: 2022-06-15
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Akt1抗体 (B-1) is rated 4.7 out of 5 by 92.
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