Date published: 2025-9-7
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p-Akt1/2/3 Antibody (C-11) - Western Blotting - Image 376603
p-Akt1/2/3 抗体 (C-11): sc-514032. SJRH30 (A), OVCAR-3 (B), Sol8 (C) 和 C2C12 (D) 全细胞裂解液中 Akt1/2/3 磷酸化的 Western 印迹分析.
p-Akt1/2/3 抗体 (C-11): sc-514032. SJRH30 (A), OVCAR-3 (B), Sol8 (C) 和 C2C12 (D) 全细胞裂解液中 Akt1/2/3 磷酸化的 Western 印迹分析.

p-Akt1/2/3 抗体 (C-11): sc-514032

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说明书
  • p-Akt1/2/3抗体(C-11)是小鼠单克隆IgG2b κ, 在173篇文献中引用，规格为200 µg/ml
  • 特异性表位，对应于含磷酸化丝氨酸473的短氨基酸序列，Akt1 human来源
  • 抗-p-Akt1/2/3 抗体 (C-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Ser 473 phosphorylated Akt1 and correspondingly Ser 474 phosphorylated Akt2 and correspondingly Ser 472 phosporylated Akt3
  • 抗p-Akt1/2/3抗体（C-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-Akt1/2/3 (C-11): sc-514032的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 p-Akt1/2/3 抗体 (C-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-Akt1/2/3 抗体 (C-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Akt 抗体包括 Akt1, Akt2 和 Akt3

p-Akt1/2/3抗体（C-11）是一种IgG2b κ小鼠单克隆p-Akt1/2/3抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Ser 473磷酸化Akt1以及相应的Ser 474磷酸化Akt2和Ser 472磷酸化Akt3。p-Akt1/2/3抗体（C-11）可以是非偶联的抗p-Akt1/2/3抗体形式，也可以是多种偶联形式的抗p-Akt1/2/3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。丝氨酸/苏氨酸激酶Akt家族包含几个成员，包括Akt1（也称为PKB或RacPK）、Akt2（也称为PKBβ或RacPK-β）和Akt3（也称为PKBγ或胸腺病毒原癌基因3），它们与蛋白激酶A和C家族具有序列同源性，并由c-Akt原癌基因编码。Akt家族的所有成员都具有血小板同源结构域。Akt1和Akt2通过PDGF刺激被激活。这种激活依赖于PDGFR-β酪氨酸残基740和751，它们与磷脂酰肌醇3-激酶（PI 3-激酶）复合物的亚基结合。胰岛素或胰岛素生长因子-1（IGF-1）对Akt1的激活导致Thr 308和Ser 473的磷酸化。在胰岛素/IGF-1刺激的细胞中，Akt蛋白通过上游激酶被磷酸化和激活，而Akt1和Akt2的激活被PI激酶抑制剂wortmannin抑制。综上所述，这些数据充分表明该蛋白是PI激酶下游的信号分子。Akt3在丝氨酸残基上被磷酸化以响应胰岛素。然而，胰岛素对Akt3的激活被蛋白激酶C的先前激活所抑制，这种机制不需要PH结构域的存在。Akt3在3T3-L1成纤维细胞、脂肪细胞和骨骼肌中表达，可能参与各种生物过程，包括脂肪细胞和肌肉分化、糖原合成、葡萄糖摄取、细胞凋亡和细胞增殖。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Akt1/2/3 抗体 (C-11) 参考文献:

  1. 细胞质Skp2表达与p-Akt1相关，并预测人类乳腺癌预后不良。  |  Liu, J., et al. 2012. PLoS One. 7: e52675. PMID: 23300741
  2. 发现4-(哌嗪-1-基)-7H-吡咯并[2,3-d]嘧啶衍生物作为Akt抑制剂。  |  Liu, Y., et al. 2016. Arch Pharm (Weinheim). 349: 356-62. PMID: 26991997
  3. Slug通过上调p21/p27蛋白和下调Wnt/β-catenin信号通路的活性，抑制人宫颈癌细胞的增殖和肿瘤形成，通过转抑制Akt1/p-Akt1表达。  |  Cui, N., et al. 2016. Oncotarget. 7: 26152-67. PMID: 27036045
  4. 槲皮素通过抑制p-Akt1、基质金属蛋白酶（MMP）MMP-2和MMP-9的表达，抑制HCCLM3细胞的迁移和侵袭。  |  Lu, J., et al. 2018. Med Sci Monit. 24: 2583-2589. PMID: 29701200
  5. P-AKT2/SPK1 (P-SPK1)和P-MEK/P-ERK细胞信号传导通路参与LPS诱导的巨噬细胞迁移。  |  Lei, Y., et al. 2019. Am J Transl Res. 11: 2725-2741. PMID: 31217849
  6. 多发性硬化症患者外周T细胞亚群中Akt1和p-Akt1的表达。  |  Oktelik, FB., et al. 2021. Acta Neurol Belg. 121: 1777-1782. PMID: 33034831
  7. 六磷酸酶 2 通过 Akt1/p-Akt1 提高宫颈癌细胞中的纤维粘连蛋白，从而促进细胞移动和远处转移。  |  Chen, Q., et al. 2021. Cancer Cell Int. 21: 600. PMID: 34758823
  8. Hexokinase 2通过激活人卵巢癌细胞中的Akt1/p-Akt1促进细胞运动和增殖。  |  Tian, X., et al. 2022. J Ovarian Res. 15: 92. PMID: 35953860
  9. p-AKT1作为激素受体阳性及人表皮生长因子受体2阳性早期乳腺癌患者的预后标志物和治疗靶标的重要性。  |  Kim, JY., et al. 2022. J Breast Cancer. 25: 387-403. PMID: 36314765
  10. IL-21/23轴通过p-Akt1信号传导调节RA CD4+ T细胞中的炎症细胞因子和RANKL表达。  |  Bhattacharya, G., et al. 2023. Front Immunol. 14: 1235514. PMID: 37809066

订购信息

产品名称产品编号规格价格数量收藏夹

p-Akt1/2/3 抗体 (C-11)

sc-514032
200 µg/ml
RMB2377.00

p-Akt1/2/3 (C-11): m-IgG Fc BP-HRP 套装

sc-526342
200 µg Ab; 10 µg BP
RMB2662.00

p-Akt1/2/3 (C-11): m-IgG2b BP-HRP 套装

sc-549715
200 µg Ab; 10 µg BP
RMB2662.00

p-Akt1/2/3 抗体 (C-11) AC

sc-514032 AC
500 µg/ml, 25% agarose
RMB3129.00

p-Akt1/2/3 抗体 (C-11) HRP

sc-514032 HRP
200 µg/ml
RMB2377.00

p-Akt1/2/3 抗体 (C-11) FITC

sc-514032 FITC
200 µg/ml
RMB2482.00

p-Akt1/2/3 抗体 (C-11) PE

sc-514032 PE
200 µg/ml
RMB2580.00

p-Akt1/2/3 抗体 (C-11) Alexa Fluor® 488

sc-514032 AF488
200 µg/ml
RMB2685.00

p-Akt1/2/3 抗体 (C-11) Alexa Fluor® 546

sc-514032 AF546
200 µg/ml
RMB2685.00

p-Akt1/2/3 抗体 (C-11) Alexa Fluor® 594

sc-514032 AF594
200 µg/ml
RMB2685.00

p-Akt1/2/3 抗体 (C-11) Alexa Fluor® 647

sc-514032 AF647
200 µg/ml
RMB2685.00

p-Akt1/2/3 抗体 (C-11) Alexa Fluor® 680

sc-514032 AF680
200 µg/ml
RMB2685.00

p-Akt1/2/3 抗体 (C-11) Alexa Fluor® 790

sc-514032 AF790
200 µg/ml
RMB2685.00

p-Akt1/2/3 (C-11) 中和勝肽

sc-514032 P
100 µg/0.5 ml
RMB511.00

HRP 의 경우 타겟 항체에 대한 specific 제품을 사용해야 하나요 ?  p-Akt 항체를 가지고 WB를 하려고 하는데, p-Akt1/2/3 Antibody (C-11) HRP 또는 m-IgGκ BP-HRP sc-516102을 사용해도 되는지요 ? 

Asked by: seong Joseph
산타크루즈바이오테크놀러지에 문의 주셔서 감사합니다.HRP 결합 1 차 항체 있으시면 ,2 차 항체 사용할 필요가 없습니다.sc-514032는 sc-516102을 사용해도 됍니다.다른 문제 있으시면 기술지원팀에 연락하셔서 전화:86 21-60936350 이메일: [email protected] 또는 온라인문의로 문의하시면 됩니다. Thank you for your question.There is no need to use 2nd antibody with HRP conjugated primary antibody.sc-51610 is a suitble 2nd antibody for sc-514032 in WB.If you have any other question ,please contact our Technical Service Department at email [email protected],call us directly at  (86 21) 6093-6350, or contact us by live chat.
Answered by: Technical Support 8
Date published: 2019-01-25

I am using p-Akt1/2/3 (C-11): sc-514032 monoclonal antibody for Western blot. How do I know if the band I am detecting is specific?

Asked by: jenni
We recommend using the blocking peptide sc-514032 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 10
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with p-Akt1/2/3 (C-11): sc-514032?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-12
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Rated 5 out of 5 by from 抗體讚這支抗體專一性高、背景低、操作穩定，適用多種實驗，是非常可靠的工具!!!!!!!!!!!!!!!!!!
Date published: 2025-07-18
Rated 4 out of 5 by from This antibody worked well in WBThis antibody worked well in WB using human macrophages
Date published: 2025-06-06
Rated 5 out of 5 by from 信号清晰且稳定我们在Western Blot实验中使用了p-Akt1/2/3抗体，结果非常出色。抗体具有很高的特异性，能够有效检测到磷酸化的Akt蛋白，信号清晰且稳定。背景噪音很低，确保了结果的准确性。该抗体在我们的实验中表现一致，可靠性高，是研究信号通路中Akt磷酸化的重要工具。
Date published: 2024-12-19
Rated 5 out of 5 by from Great features for western blotThe antibody can give a nice clean band. It is a good antibody.
Date published: 2024-07-03
Rated 5 out of 5 by from 抗体质量很好Santa Cruz Biotechnology家的抗体跑完western Blot后条带很清晰，杂带少，值得推荐
Date published: 2024-06-16
Rated 4 out of 5 by from good antibodygood antibody clean and strong signal in western blot or IF
Date published: 2021-11-15
Rated 5 out of 5 by from Very goodThe antibody was used for Western Blot in breast carcinoma cells, it works very well at a concentration of 1: 100
Date published: 2020-10-16
Rated 4 out of 5 by from Very good antibodyThe antibody was used for Western Blot with breast carcinoma cells, it works very well at a concentration of 1: 200.
Date published: 2018-11-22
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p-Akt1/2/3 抗体 (C-11) is rated 4.5 out of 5 by 62.
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