Date published: 2025-9-7
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p-Akt1/2/3 Antibody (B-5) - Western Blotting - Image 376860
p-Akt1/2/3 抗体 (B-5): sc-271966. 对 Jurkat (A, C) 和钙霉素处理过的 Jurkat (B, D) 全细胞裂解液中 p-Akt1/2/3 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Akt1/2/3 (A-12): sc-271966 (A, B) 和 Akt1 (B-1): sc-5298 (C, D).
p-Akt1/2/3 抗体 (B-5): sc-271966. 对 Jurkat (A, C) 和钙霉素处理过的 Jurkat (B, D) 全细胞裂解液中 p-Akt1/2/3 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Akt1/2/3 (A-12): sc-271966 (A, B) 和 Akt1 (B-1): sc-5298 (C, D).

p-Akt1/2/3 抗体 (B-5): sc-271966

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说明书
  • p-Akt1/2/3抗体(B-5)是小鼠单克隆IgG2b κ, 在114篇文献中引用，规格为200 µg/ml
  • p-Akt1/2/3 抗体 (B-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的Thr 308 phosphorylated Akt1 and correspondingly Thr 309 phosphorylated Akt2 and correspondingly Thr 305 phosphorylated Akt3; 也和以下物种反应，包括： equine and avian
  • 抗p-Akt1/2/3抗体（B-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-Akt1/2/3 (B-5): sc-271966的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是p-Akt1/2/3 抗体 (B-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与p-Akt1/2/3 抗体 (B-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
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研究信息

関連項目

此外 Akt 抗体包括 Akt1, Akt2 和 Akt3

p-Akt1/2/3抗体（B-5）是一种IgG2b κ小鼠单克隆p-Akt1/2/3抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Thr 308磷酸化Akt1以及相应的Thr 309磷酸化Akt2和Thr 305磷酸化Akt3。p-Akt1/2/3抗体（B-5）可以是非偶联的抗p-Akt1/2/3抗体形式，也可以是多种偶联形式的抗p-Akt1/2/3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。丝氨酸/苏氨酸激酶Akt家族包含几个成员，包括Akt1（也称为PKB或RacPK）、Akt2（也称为PKBβ或RacPK-β）和Akt3（也称为PKBγ或胸腺病毒原癌基因3），它们与蛋白激酶A和C家族具有序列同源性，并由c-Akt原癌基因编码。Akt家族的所有成员都具有血小板同源结构域。Akt1和Akt2通过PDGF刺激被激活。这种激活依赖于PDGFR-β酪氨酸残基740和751，它们与磷脂酰肌醇3-激酶（PI 3-激酶）复合物的亚基结合。胰岛素或胰岛素生长因子-1（IGF-1）对Akt1的激活导致Thr 308和Ser 473的磷酸化。在胰岛素/IGF-1刺激的细胞中，Akt蛋白通过上游激酶被磷酸化和激活，而Akt1和Akt2的激活被PI激酶抑制剂wortmannin抑制。综上所述，这些数据充分表明该蛋白是PI激酶下游的信号分子。Akt3在丝氨酸残基上被磷酸化以响应胰岛素。然而，胰岛素对Akt3的激活被蛋白激酶C的先前激活所抑制，这种机制不需要PH结构域的存在。Akt3在3T3-L1成纤维细胞、脂肪细胞和骨骼肌中表达，可能参与各种生物过程，包括脂肪细胞和肌肉分化、糖原合成、葡萄糖摄取、细胞凋亡和细胞增殖。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Akt1/2/3 抗体 (B-5) 参考文献:

  1. 细胞质Skp2表达与p-Akt1相关，并预测人类乳腺癌预后不良。  |  Liu, J., et al. 2012. PLoS One. 7: e52675. PMID: 23300741
  2. 发现4-(哌嗪-1-基)-7H-吡咯并[2,3-d]嘧啶衍生物作为Akt抑制剂。  |  Liu, Y., et al. 2016. Arch Pharm (Weinheim). 349: 356-62. PMID: 26991997
  3. Slug通过上调p21/p27蛋白和下调Wnt/β-catenin信号通路的活性，抑制人宫颈癌细胞的增殖和肿瘤形成，通过转抑制Akt1/p-Akt1表达。  |  Cui, N., et al. 2016. Oncotarget. 7: 26152-67. PMID: 27036045
  4. 槲皮素通过抑制p-Akt1、基质金属蛋白酶（MMP）MMP-2和MMP-9的表达，抑制HCCLM3细胞的迁移和侵袭。  |  Lu, J., et al. 2018. Med Sci Monit. 24: 2583-2589. PMID: 29701200
  5. P-AKT2/SPK1 (P-SPK1)和P-MEK/P-ERK细胞信号传导通路参与LPS诱导的巨噬细胞迁移。  |  Lei, Y., et al. 2019. Am J Transl Res. 11: 2725-2741. PMID: 31217849
  6. 多发性硬化症患者外周T细胞亚群中Akt1和p-Akt1的表达。  |  Oktelik, FB., et al. 2021. Acta Neurol Belg. 121: 1777-1782. PMID: 33034831
  7. 六磷酸酶 2 通过 Akt1/p-Akt1 提高宫颈癌细胞中的纤维粘连蛋白，从而促进细胞移动和远处转移。  |  Chen, Q., et al. 2021. Cancer Cell Int. 21: 600. PMID: 34758823
  8. Hexokinase 2通过激活人卵巢癌细胞中的Akt1/p-Akt1促进细胞运动和增殖。  |  Tian, X., et al. 2022. J Ovarian Res. 15: 92. PMID: 35953860
  9. p-AKT1作为激素受体阳性及人表皮生长因子受体2阳性早期乳腺癌患者的预后标志物和治疗靶标的重要性。  |  Kim, JY., et al. 2022. J Breast Cancer. 25: 387-403. PMID: 36314765
  10. IL-21/23轴通过p-Akt1信号传导调节RA CD4+ T细胞中的炎症细胞因子和RANKL表达。  |  Bhattacharya, G., et al. 2023. Front Immunol. 14: 1235514. PMID: 37809066

订购信息

产品名称产品编号规格价格数量收藏夹

p-Akt1/2/3 抗体 (B-5)

sc-271966
200 µg/ml
RMB2377.00

p-Akt1/2/3 (B-5): m-IgG2b BP-HRP 套装

sc-548711
200 µg Ab; 10 µg BP
RMB2662.00

p-Akt1/2/3 抗体 (B-5) AC

sc-271966 AC
500 µg/ml, 25% agarose
RMB3129.00

p-Akt1/2/3 抗体 (B-5) HRP

sc-271966 HRP
200 µg/ml
RMB2377.00

p-Akt1/2/3 抗体 (B-5) FITC

sc-271966 FITC
200 µg/ml
RMB2482.00

p-Akt1/2/3 抗体 (B-5) PE

sc-271966 PE
200 µg/ml
RMB2580.00

p-Akt1/2/3 抗体 (B-5) Alexa Fluor® 488

sc-271966 AF488
200 µg/ml
RMB2685.00

p-Akt1/2/3 抗体 (B-5) Alexa Fluor® 546

sc-271966 AF546
200 µg/ml
RMB2685.00

p-Akt1/2/3 抗体 (B-5) Alexa Fluor® 594

sc-271966 AF594
200 µg/ml
RMB2685.00

p-Akt1/2/3 抗体 (B-5) Alexa Fluor® 647

sc-271966 AF647
200 µg/ml
RMB2685.00

p-Akt1/2/3 抗体 (B-5) Alexa Fluor® 680

sc-271966 AF680
200 µg/ml
RMB2685.00

p-Akt1/2/3 抗体 (B-5) Alexa Fluor® 790

sc-271966 AF790
200 µg/ml
RMB2685.00

p-Akt1/2/3 (B-5) 中和勝肽

sc-271966 P
100 µg/0.5 ml
RMB511.00

Have you or do you know of western data for use of sc-271966 to detect mouse brain p-Akt1/2/3?

Asked by: Billf
Thank you for your question. We have not tested in mouse brain and do not have any publications for mouse brain. If you happen to purchase the antibody we have a one year warranty from date of purchase. We have a antibody sample program please contact technical service to get more information. Please email [email protected] or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-05-18

What application is the blocking peptide sc-271966 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great for WBThe antibody is good for western blot. The band is single and clear.
Date published: 2024-03-19
Rated 5 out of 5 by from p-AKT antibodyThe antibody is good on western blot. The band is single and clear.
Date published: 2023-08-06
Rated 5 out of 5 by from B5 mAb P-AKTGood antibody for cell staining, P-AKT in green shows well in our cells.
Date published: 2022-07-22
Rated 5 out of 5 by from Good!It can be used for detection in human cacer cell lines. Although it is a very good antibody for WB, a non-specific band was also seen.
Date published: 2021-02-20
Rated 2 out of 5 by from Funziona solo su PBMC non su linee di CancroPrimary antibody pAKT 1/2/3 (B-5) sc-271966 was used diluted 1:500 according to the manufacturer’s recommendations (for Western Blotting dilution range 1:100-1:1000), in BSA 5% for pAKT, 1%. Membrane of PVDF has been incubated overnight at 4°C and then it has been probed for 1h at RT with Santa Cruz secondary antibodies diluted 1:5000 in BSA 1% for pAKT. We have tested this antibody on two different sets of samples: in six cancer cell lines and in human activated PBMCs. Moreover, even if we tested the antibody at higher concentration, we only obtained a higher background but not an increase in the specific protein signal; therefore we show only the blots obtained after the incubation with antibodies prepared as described above. In conclusion: • Strangely, pAKT did not seem to work on these cancer cell lines where there should be high levels of protein, as we know from our data and from literature. Indeed we tested another pAKT antibody (Cell Signaling S473 (D9E) 4060S) on the same blot and it was successful to detect the specific protein (left panel, middle). However it did not work on these cancer cell lines, the antibody pAKT 1/2/3 (B-5) sc-271966 worked well on lymphocytes; All the membranes have been probed with a normalizer and each gel has been stained with Coomassie Brillant Blue, shown on the right side of each panel, in order to demonstrate that the samples ran well and that the proteins are present on the SDS-PAGE.
Date published: 2019-11-26
Rated 4 out of 5 by from p-Akt1/2/3 Antibody (B-5)p-Akt1/2/3 Antibody (B-5) is good quality and high stability for western blotting (dilute factor: 1:1000)
Date published: 2019-05-17
Rated 4 out of 5 by from Good antibodiesThe antibodies works good. I used this antibody 1:1000 on Western blot.
Date published: 2018-06-29
Rated 4 out of 5 by from Great productI got a free sample from company. In Western Blot antibodies works in dilution 1:5000.
Date published: 2018-06-10
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p-Akt1/2/3 抗体 (B-5) is rated 4.2 out of 5 by 40.
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