Date published: 2025-9-8

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OTX2 抗体 (D-8): sc-514195

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说明书
  • OTX2 抗体 D-8 是小鼠单克隆 IgG1 κ,OTX2抗体, 在20篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列115-259位于human物种的OTX2的C-terminus附近
  • OTX2 抗体 (D-8) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 OTX2
  • 抗OTX2抗体(D-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取OTX2 (D-8): sc-514195免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是OTX2 Antibody (D-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与OTX2 Antibody (D-8) 打包提供(请参阅下面的订购信息)。

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関連項目

OTX2抗体(D-8)是一种IgG1 κ型小鼠单克隆OTX2抗体(也称为OTX2抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人的OTX2蛋白。OTX2抗体(D-8)既有非偶联的抗OTX2抗体形式,也有多种偶联形式的抗OTX2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。转录因子OTX1和OTX2是果蝇orthodenticle(OTD)的两个小鼠同源物,显示出有限的氨基酸序列差异。OTX1和OTX2在大脑发育早期和后期所需的事件中发挥着重要作用,因为它们参与大脑的诱导、特化和区域化过程。OTX1参与皮质发生、感觉器官发育和垂体功能,而OTX2在发育早期是必需的,对于正确的前神经板特化和原条的组织至关重要。OTX2也是早期神经外胚层特化所必需的,神经外胚层注定要成为前中脑,而OTX1和OTX2通过剂量依赖性机制共同作用于发育中的大脑的构图。依赖于OTX蛋白精确阈值的分子机制对于峡部区域的正确定位和前脑构图是必需的。编码OTX1和OTX2的基因分别定位于人类染色体2p13和14q21-q22。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

OTX2 抗体 (D-8) 参考文献:

  1. Otx基因和大脑形态发生的基因控制。  |  Acampora, D., et al. 1999. Mol Cell Neurosci. 13: 1-8. PMID: 10049527
  2. 转录控制的差异是产生 Otx1-/- 和 Otx2-/- 不同表型的主要分子事件。  |  Acampora, D., et al. 1999. Development. 126: 1417-26. PMID: 10068635
  3. TINS 讲座。了解 Otx1 和 Otx2 在控制大脑形态发生中的作用。  |  Acampora, D. and Simeone, A. 1999. Trends Neurosci. 22: 116-22. PMID: 10199636
  4. 小鼠内耳的正常发育需要 Otx1 和 Otx2 的活性。  |  Morsli, H., et al. 1999. Development. 126: 2335-43. PMID: 10225993
  5. 皮质形成和大脑发育中的 Otx 基因  |  Acampora, D., et al. 1999. Cereb Cortex. 9: 533-42. PMID: 10498271
  6. Otx同源结构域蛋白在头部发育中的遗传和分子作用  |  Acampora, D., et al. 2000. Gene. 246: 23-35. PMID: 10767524
  7. 早期生活压力通过腹侧被盖区 OTX2 使小鼠终生易受压力影响。  |  Peña, CJ., et al. 2017. Science. 356: 1185-1188. PMID: 28619944
  8. MDIG 介导的 H3K9me3 去甲基化可通过激活 OTX2 上调 Myc,促进肝脏再生。  |  Du, J., et al. 2023. Signal Transduct Target Ther. 8: 351. PMID: 37709738
  9. O-GlcNAcylation 可调节 OTX2 的蛋白稳态。  |  Wulff-Fuentes, E., et al. 2023. iScience. 26: 108184. PMID: 38026167
  10. 第3组髓母细胞瘤干细胞程序由OTX2介导的替代剪接维持。  |  Saulnier, O., et al. 2024. Nat Cell Biol. 26: 1233-1246. PMID: 39025928
  11. 人类 EMX 和 OTX 基因的染色体位置。  |  Kastury, K., et al. 1994. Genomics. 22: 41-5. PMID: 7959790
  12. Otx2和Otx1在喙头发育过程中的功能等同性  |  Suda, Y., et al. 1999. Development. 126: 743-57. PMID: 9895322

订购信息

产品名称产品编号规格价格数量收藏夹

OTX2 抗体 (D-8)

sc-514195
200 µg/ml
RMB2377.00

OTX2 (D-8): m-IgG Fc BP-HRP 套装

sc-531061
200 µg Ab; 10 µg BP
RMB2662.00

OTX2 (D-8): m-IgGκ BP-HRP 套装

sc-524644
200 µg Ab, 40 µg BP
RMB2662.00

OTX2 (D-8): m-IgG1 BP-HRP 套装

sc-544378
200 µg Ab; 20 µg BP
RMB2662.00

OTX2 抗体 (D-8) AC

sc-514195 AC
500 µg/ml, 25% agarose
RMB3129.00

OTX2 抗体 (D-8) HRP

sc-514195 HRP
200 µg/ml
RMB2377.00

OTX2 抗体 (D-8) FITC

sc-514195 FITC
200 µg/ml
RMB2482.00

OTX2 抗体 (D-8) PE

sc-514195 PE
200 µg/ml
RMB2580.00

OTX2 抗体 (D-8) Alexa Fluor® 488

sc-514195 AF488
200 µg/ml
RMB2685.00

OTX2 抗体 (D-8) Alexa Fluor® 546

sc-514195 AF546
200 µg/ml
RMB2685.00

OTX2 抗体 (D-8) Alexa Fluor® 594

sc-514195 AF594
200 µg/ml
RMB2685.00

OTX2 抗体 (D-8) Alexa Fluor® 647

sc-514195 AF647
200 µg/ml
RMB2685.00

OTX2 抗体 (D-8) Alexa Fluor® 680

sc-514195 AF680
200 µg/ml
RMB2685.00

OTX2 抗体 (D-8) Alexa Fluor® 790

sc-514195 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-514195: OTX2 (D-8) mouse monoclonal antibody will work?

Asked by: jenniferc
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 1 out of 5 by from Doesn't work for mouse brain section IFI tested the sample version of the antibody on mouse brain sections (two different genotypes). Unfortunately it did not work in either case.
Date published: 2018-01-17
Rated 4 out of 5 by from Works well with antigen retrievalThis antibody worked well on fixed frozen sections with antigen retrieval.
Date published: 2017-07-26
Rated 4 out of 5 by from The antibody worksThis otx2 antibody works well. The target band is clear and sharp, very easy to identify.
Date published: 2017-03-26
Rated 5 out of 5 by from Excellent antibody for WBThis antibody worked for my WB. Good antibody with less background
Date published: 2017-03-15
Rated 5 out of 5 by from outstandingI use this antibody to detect the expression of OTX2 in human stem cell after certain induction as a neural marker. Antibody produced great results by WB ,Highly recommended!
Date published: 2017-02-13
Rated 5 out of 5 by from Satisfactory Western Blot data of OTX2 expressionSatisfactory Western Blot data of OTX2 expression in Jurkat, Hep G2 and HeLa nuclear extracts and U-87 MG whole cell lysate . -SCBT QC
Date published: 2015-01-13
Rated 5 out of 5 by from Nice Western Blot data of OTX2 expression in NTERANice Western Blot data of OTX2 expression in NTERA-2 cl.D1 whole cell lysate. -SCBT QC
Date published: 2013-10-23
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OTX2 抗体 (D-8) is rated 4.1 out of 5 by 7.
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