Date published: 2025-9-8
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OPA1 Antibody (D-9) - Western Blotting - Image 294530
OPA1 抗体 (D-9): sc-393296. 拉莫斯 (A), NIH/3T3 (B) 和 HeLa (C) 全细胞裂解液以及大鼠大脑 (D) 和人类海马 (E) 组织提取物中 OPA1 表达的 Western 印迹分析.
OPA1 Antibody (D-9) - Western Blotting - Image 294530
OPA1 抗体 (D-9): sc-393296. 拉莫斯 (A), NIH/3T3 (B) 和 HeLa (C) 全细胞裂解液以及大鼠大脑 (D) 和人类海马 (E) 组织提取物中 OPA1 表达的 Western 印迹分析.
OPA1 Antibody (D-9) - Western Blotting - Image 381573
OPA1 抗体 (D-9): sc-393296. HeLa 全细胞裂解液中 OPA1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
OPA1 Antibody (D-9) - Western Blotting - Image 398908
OPA1 抗体 (D-9) HRP: sc-393296 HRP. HeLa (A), NRK (B) 和 RAT2 (C) 全细胞裂解液中 OPA1 表达的直接 western 印迹分析.
OPA1 抗体 (D-9): sc-393296. 拉莫斯 (A), NIH/3T3 (B) 和 HeLa (C) 全细胞裂解液以及大鼠大脑 (D) 和人类海马 (E) 组织提取物中 OPA1 表达的 Western 印迹分析.

OPA1 抗体 (D-9): sc-393296

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说明书
  • OPA1 抗体 D-9 是小鼠单克隆 IgG1 κ，OPA1抗体, 在62篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列647-780位于human物种的OPA1的内部区域内
  • OPA1 抗体 (D-9) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 OPA1; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗OPA1抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取OPA1 (D-9): sc-393296的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是OPA1 Antibody (D-9) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与OPA1 Antibody (D-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

OPA抗体

OPA1抗体（D-9）是一种IgG1 κ小鼠单克隆OPA1抗体（也称为Dynamin样120 KDa线粒体蛋白抗体、Dynamin样鸟苷三磷酸酶抗体、视神经萎缩1（常染色体显性）抗体、线粒体Dynamin样GTP酶抗体、视神经萎缩蛋白1抗体、MGM1抗体、NPG抗体、NTG抗体或BERHS抗体），可通过WB, IP, IF 和 ELISA检测小鼠、大鼠和人类来源的OPA1蛋白。OPA1抗体（D-9）既有非偶联的抗OPA1抗体形式，也有多种偶联形式的抗OPA1抗体，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。OPA1（视神经萎缩1基因蛋白）属于Dynamin家族。编码OPA1的基因定位于3q29，靶向线粒体，参与线粒体生物发生。OPA1的缺陷是视神经萎缩1型的原因。OPA1主要在视网膜中表达，但也可在脑、睾丸、心脏和骨骼肌中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

OPA1 抗体 (D-9) 参考文献:

  1. 编码线粒体达因明相关蛋白的核基因 OPA1 在显性视神经萎缩中发生突变。  |  Delettre, C., et al. 2000. Nat Genet. 26: 207-10. PMID: 11017079
  2. 显性视神经萎缩症中 OPA1 基因突变的范围, 频率和渗透性。  |  Toomes, C., et al. 2001. Hum Mol Genet. 10: 1369-78. PMID: 11440989
  3. OPA1 基因的突变谱和剪接变体。  |  Delettre, C., et al. 2001. Hum Genet. 109: 584-91. PMID: 11810270
  4. 线粒体中动态相关蛋白 OPA1 异构体的不同亚定位。  |  Satoh, M., et al. 2003. Biochem Biophys Res Commun. 300: 482-93. PMID: 12504110
  5. 哺乳动物线粒体裂变和融合介质 Fis1, Drp1 和 Opa1 在细胞凋亡中的作用。  |  Lee, YJ., et al. 2004. Mol Biol Cell. 15: 5001-11. PMID: 15356267
  6. OPA1 需要丝裂蛋白 1 来促进线粒体融合。  |  Cipolat, S., et al. 2004. Proc Natl Acad Sci U S A. 101: 15927-32. PMID: 15509649
  7. OPA1 与常染色体显性视神经萎缩有关，在人脑中广泛表达。  |  Bette, S., et al. 2005. Acta Neuropathol. 109: 393-9. PMID: 15700187
  8. 将显性视神经萎缩基因座（OPA1）细化为染色体 3q28-3q29 上的 1.4 厘米区间，位于 3-Mb YAC contig 内。  |  Jonasdottir, A., et al. 1997. Hum Genet. 99: 115-20. PMID: 9003507

订购信息

产品名称产品编号规格价格数量收藏夹

OPA1 抗体 (D-9)

sc-393296
200 µg/ml
RMB2377.00

OPA1 (D-9): m-IgG Fc BP-HRP 套装

sc-530422
200 µg Ab; 10 µg BP
RMB2662.00

OPA1 (D-9): m-IgGκ BP-HRP 套装

sc-523893
200 µg Ab, 40 µg BP
RMB2662.00

OPA1 抗体 (D-9) AC

sc-393296 AC
500 µg/ml, 25% agarose
RMB3129.00

OPA1 抗体 (D-9) HRP

sc-393296 HRP
200 µg/ml
RMB2377.00

OPA1 抗体 (D-9) FITC

sc-393296 FITC
200 µg/ml
RMB2482.00

OPA1 抗体 (D-9) PE

sc-393296 PE
200 µg/ml
RMB2580.00

OPA1 抗体 (D-9) Alexa Fluor® 488

sc-393296 AF488
200 µg/ml
RMB2685.00

OPA1 抗体 (D-9) Alexa Fluor® 546

sc-393296 AF546
200 µg/ml
RMB2685.00

OPA1 抗体 (D-9) Alexa Fluor® 594

sc-393296 AF594
200 µg/ml
RMB2685.00

OPA1 抗体 (D-9) Alexa Fluor® 647

sc-393296 AF647
200 µg/ml
RMB2685.00

OPA1 抗体 (D-9) Alexa Fluor® 680

sc-393296 AF680
200 µg/ml
RMB2685.00

OPA1 抗体 (D-9) Alexa Fluor® 790

sc-393296 AF790
200 µg/ml
RMB2685.00

I am currently looking for antibodies for OPA1 for immunofluorescence. I would like to ask if it is possible to get a trial sample to try? We have OPA1 ko cells so if it works, I could upload some photos and protocols to share. Many thanks in advance!

Asked by: Xiaotong
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2020-07-24

As I am unhappy with the current OPA1-antibody we are using (other manufacturer), I am looking to buy a new one. Would it be possible to get a free sample of this antibody for testing?

Asked by: Christiaan
Thank you for your question. Please contact Technical Service for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-02-06

8% separation gel, first antibody 1:100, 4℃，overnight. second antibody 1:1000,room temperature, 1 hour. But I think the band (130-250kDa) is not right.

Asked by: Anonymous
Thank you for your question. Please contact your local distributor or Technical Service directly for assistance regarding your WB results, we are happy to discuss your results with you.
Answered by: Tech Service
Date published: 2019-04-26

what buffer does the opa1 antibody work best in? I tried probing a blot of mouse heart tissue with opa1 at 1:1000 in 5%milk/ TBST and the blot is there is a lot of background. I checked the publications cited and they lack the buffer details

Asked by: Dr C
Thank you for your question. The optimal blocking buffer and antibody diluent depends on which type of Western blot detection method you are using. If you are using an HRP conjugated secondary for chemiluminescent detection, then we recommend Blotto, or 5% nonfat milk in TBST (1X TBST + 0.05% Tween-20) for blocking, and for incubating both primary and secondary antibodies. If you are using a fluorescent or NIR (Near-Infrared) detection method, for optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF: sc-516541, and UltraCruz® Blocking Reagent: sc-516214 for all blocking, primary antibody and secondary antibody incubations.
Answered by: Technical Support
Date published: 2022-04-27

I would like to know your OPA1 antibody (D9): sc-393296 could use with immunofluorescence? How long to be shipped to Korea, the volume and the price to my university in Korea. Thank you very much!

Asked by: Trang
Thank you for your question. We have not yet tested this mouse monoclonal antibody, OPA1 (D-9): sc-393296, in immunofluorescent staining applications. However, based on the antibody's performance in other applications, we do recommend and warranty the antibody for IF, or immunofluorescent staining of fresh or frozen tissues or cells, fixed in methanol or acetone. Please contact one of our authorized distributors in Korea to inquire about shipping times and to receive a quote to ship to a University in Korea. A link is provided below to search for our distributors. https://www.scbt.com/scbt/resources/distributors For a limited time, we are offering a free 10 µg sample of this antibody. Please contact us if you would like to request a sample to test in your application. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-25

Can OPA1 Antibody (D-9): sc-393296 be used for detection of OPA1 of chicken origin?

Asked by: jenni
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 1 out of 5 by from it does not worksMaybe it works in lysates from tissue but does not works in cell lysates, not even at 1:100.
Date published: 2023-04-24
Rated 1 out of 5 by from very poor quality.Purchased this antibody for studying mitochondrial dynamics, very faint signal and a huge background noise, I used 1/100 dilution. after 4 times trying with different gels and samples, I gave up.
Date published: 2021-04-01
Rated 4 out of 5 by from goodSingle extremely weak band detected with muscle cells, but strong single in muscle tissue.
Date published: 2020-02-06
Rated 4 out of 5 by from Good Inner mitochondrial membrane protein markerCan be used in 1:500 dilution for western blotting to look at mouse mitochondrial extracts.
Date published: 2019-10-05
Rated 5 out of 5 by from good signalI loaded total homogenate from mouse brain 5-10-20-30 ug, good signal with 20 and 30 ug. I recommend this antibody for WB.
Date published: 2019-01-28
Rated 3 out of 5 by from Not quite strong band1:200 diluted for wb of rat protein,the band is not quite clear
Date published: 2018-06-12
Rated 3 out of 5 by from WBTested in SHSY-5Y human neuroblastoma cells (~15-20ug/lane), dilution 1:1000 in 3% milk in TBST. Got a single clear band at expected size after very long exposure.
Date published: 2018-05-15
Rated 4 out of 5 by from Great featuresI bought it a month ago, it works well as I recent run in WB.
Date published: 2017-10-30
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OPA1 抗体 (D-9) is rated 4.0 out of 5 by 22.
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