Date published: 2025-9-8

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Op18 抗体 (E-3): sc-55531

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说明书
  • Op18 抗体 E-3 是小鼠单克隆 IgG2b κ,Op18抗体, 在21篇文献中引用,规格为200 µg/ml
  • 与氨基酸1-149结合,代表全长Op18,human起源
  • Op18 抗体 (E-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Op18
  • 抗Op18抗体(E-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Op18 (E-3): sc-55531免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRP是Op18 Antibody (E-3) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与Op18 Antibody (E-3) 打包提供(请参阅下面的订购信息)。

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関連項目

Op18 抗体(E-3)是一种小鼠单克隆 IgG2b kappa 轻链抗体,可通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附试验(ELISA)检测小鼠、大鼠和人源的 Op18 蛋白。抗 Op18 抗体 (E-3) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Op18 又称肿瘤蛋白 18、stathmin、prosolin 或 metablastin,是一种高度保守的、与微管蛋白相关的细胞内磷蛋白,在调节微管动力学方面起着关键作用。Op18 调节微管稳定性的能力对细胞正常分裂和分化至关重要,因此 Op18 在各种细胞过程中扮演着重要角色。值得注意的是,Op18 会发生多种翻译后修饰,包括磷酸化,这会显著影响其活性以及与其他蛋白质的相互作用。这种调控在癌症中尤为重要,因为 Op18 的异常表达和修饰与多种恶性肿瘤有关,包括乳腺癌和急性白血病。Op18在这些癌症中的存在凸显了其作为生物标记物和治疗靶点的潜力,突出了了解Op18在正常和病理状态下的功能和调控的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Op18 抗体 (E-3)

sc-55531
200 µg/ml
RMB2377.00

Op18 (E-3): m-IgG Fc BP-HRP 套装

sc-525634
200 µg Ab; 10 µg BP
RMB2662.00

Op18 (E-3): m-IgG2b BP-HRP 套装

sc-549600
200 µg Ab; 10 µg BP
RMB2662.00

Op18 抗体 (E-3) AC

sc-55531 AC
500 µg/ml, 25% agarose
RMB3129.00

Op18 抗体 (E-3) HRP

sc-55531 HRP
200 µg/ml
RMB2377.00

Op18 抗体 (E-3) FITC

sc-55531 FITC
200 µg/ml
RMB2482.00

Op18 抗体 (E-3) PE

sc-55531 PE
200 µg/ml
RMB2580.00

Op18 抗体 (E-3) Alexa Fluor® 488

sc-55531 AF488
200 µg/ml
RMB2685.00

Op18 抗体 (E-3) Alexa Fluor® 546

sc-55531 AF546
200 µg/ml
RMB2685.00

Op18 抗体 (E-3) Alexa Fluor® 594

sc-55531 AF594
200 µg/ml
RMB2685.00

Op18 抗体 (E-3) Alexa Fluor® 647

sc-55531 AF647
200 µg/ml
RMB2685.00

Op18 抗体 (E-3) Alexa Fluor® 680

sc-55531 AF680
200 µg/ml
RMB2685.00

Op18 抗体 (E-3) Alexa Fluor® 790

sc-55531 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with Op18 (E-3): sc-55531 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from yes in WB no in IFGood staining on WB of human cheratinocytes. No immunofluorescence staining on PFA fixed cells.
Date published: 2018-12-12
Rated 4 out of 5 by from Good for IFThis Op18 Antibody (E-3), sc-55531, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 1 out of 5 by from Didn't work-Western BlotWe tried this sample when we ran out of our regular anti-stathmin antibody that we order from another company and it didn't work on our A20 lysates
Date published: 2017-08-24
Rated 4 out of 5 by from Band seen in WB using Jurkat whole cell lysateBand seen in WB using Jurkat whole cell lysate. -SCBT QC
Date published: 2015-06-17
Rated 4 out of 5 by from Publishable data in WB using mouse CCLPublishable data in WB using mouse CCL-247 tumor xenografts -SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmicBeautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-27
Rated 4 out of 5 by from Publishable data in WB using EpsteinPublishable data in WB using Epstein-Barr virus CNE2E cell,CNE2 cell lines. -SCBT Publication Review
Date published: 2015-03-15
Rated 4 out of 5 by from Good band detected in WB using KGood band detected in WB using K-562 whole cell lysate. -SCBT QC
Date published: 2015-02-24
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Op18 抗体 (E-3) is rated 3.6 out of 5 by 8.
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