Date published: 2025-9-8

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O-GlcNAc transferase 抗体 (A-6): sc-74547

5.0(5)
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说明书
  • O-GlcNAc transferase抗体(A-6)是小鼠单克隆IgG2a κ, 在5篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1-300,在O-GlcNAc transferase的human起源的N-terminus处进行映射
  • 推荐用于 mouse, rat 和 human 来源的O-GlcNAc transferase WB, IP, IF 和 ELISA检测; 也和以下物种反应,包括: 和 equine, canine and bovine
  • 看 O-GlcNAc transferase (F-12): sc-74546,可查看有标记的 O-GlcNAc transferase 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP是O-GlcNAc transferase Antibody (A-6) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与O-GlcNAc transferase Antibody (A-6) 搭配使用(请参阅下面的订购信息)。

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関連項目

O-连接N-乙酰葡糖胺(O-GlcNAc)转移酶(也称为OGT)催化单个N-乙酰葡糖胺以O-糖苷键连接到丝氨酸或苏氨酸残基上。由于磷酸化和糖基化都竞争相似的丝氨酸或苏氨酸残基,因此这两个过程可能会竞争位点,或者它们可能会通过空间位阻或静电效应改变附近位点的底物特异性。O-GlcNAc转移酶已从大鼠肝脏中纯化出来。它以异源三聚体复合物的形式存在,具有两个相同分子量的亚基和一个较短的亚基。这两种多肽是相关的;短亚基带要么是多肽的蛋白水解产物,要么是替代翻译起始位点的产物。O-GlcNAc转移酶以多种转录本的形式表达,这些转录本在不同的人体组织中以不同的数量存在,其中在胰腺中的表达水平最高。表达大鼠O-GlcNAc转移酶的人类细胞的免疫荧光表明,它存在于细胞核和细胞质中。表达O-GlcNAc转移酶的HeLa细胞在长时间孵育过程中存活率不佳,这表明这种蛋白质可能对细胞有毒。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

O-GlcNAc transferase 抗体 (A-6)

sc-74547
200 µg/ml
RMB2377.00

O-GlcNAc transferase (A-6): m-IgG Fc BP-HRP 套装

sc-539543
200 µg Ab; 10 µg BP
RMB2662.00

Are there any issues with using sc-74547: O-GlcNAc transferase (A-6) mouse monoclonal primary antibody on mouse samples?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-74547 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from perfect!Clear bands can be observed in WB when PC12 cells were stimulated in different lengths of time.
Date published: 2017-09-25
Rated 5 out of 5 by from Published data for WB in HEK293T and HeLa cellsPublished data for WB in HEK293T and HeLa cells. -SCBT Publication Review
Date published: 2015-06-25
Rated 5 out of 5 by from Published data for IF in human U2OS andPublished data for IF in human U2OS and fibroblast cells. -SCBT Publication Review
Date published: 2015-04-11
Rated 5 out of 5 by from Band of interest observed in WB in SKBand of interest observed in WB in SK-N-SH whole cell lysate. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Clear band with good specificity in WB in SKClear band with good specificity in WB in SK-N-MC nuclear extract and SK-N-MC, A549, c4 and rat PBL whole cell lysates. -SCBT QC
Date published: 2013-12-19
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O-GlcNAc transferase 抗体 (A-6) is rated 5.0 out of 5 by 5.
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