Date published: 2025-9-7
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O-GlcNAc transferase Antibody (F-12) - Western Blotting - Image 354324
O-GlcNAc 转移酶抗体 (F-12): sc-74546. NIH/3T3 (A), F9 (B), I-11. 15 (C) 和 C2C12 (D) 全细胞裂解液中 O-GlcNAc 转移酶表达的 Western 印迹分析.
O-GlcNAc 转移酶抗体 (F-12): sc-74546. NIH/3T3 (A), F9 (B), I-11. 15 (C) 和 C2C12 (D) 全细胞裂解液中 O-GlcNAc 转移酶表达的 Western 印迹分析.

O-GlcNAc transferase 抗体 (F-12): sc-74546

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说明书
  • O-GlcNAc transferase 抗体 F-12 是小鼠单克隆 IgG2a κ，O-GlcNAc transferase抗体, 在36篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-300，在O-GlcNAc transferase的human起源的N-terminus处进行映射
  • O-GlcNAc transferase 抗体 (F-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 O-GlcNAc transferase
  • 抗O-GlcNAc transferase抗体（F-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取O-GlcNAc transferase (F-12): sc-74546的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 O-GlcNAc transferase 抗体 (F-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与O-GlcNAc transferase 抗体 (F-12) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 GlcNAc transferase 抗体包括 HEC-GlcNAc6ST

O-GlcNAc转移酶抗体（F-12）是一种IgG2a κ小鼠单克隆O-GlcNAc转移酶抗体（也称为OGT抗体、丝氨酸/苏氨酸N-乙酰葡糖胺糖基转移酶抗体、OGTase抗体、O-GlcNAc转移酶抗体或O-连接N-乙酰葡糖胺（GlcNAc）转移酶抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的O-GlcNAc转移酶蛋白。O-GlcNAc转移酶抗体（F-12）既可作为非共轭抗O-GlcNAc转移酶抗体形式提供，也可作为多种共轭形式的抗O-GlcNAc转移酶抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。O-连接N-乙酰葡糖胺（O-GlcNAc）转移酶（也称为OGT）催化单个N-乙酰葡糖胺以O-糖苷键连接到丝氨酸或苏氨酸残基上。由于磷酸化和糖基化都竞争相似的丝氨酸或苏氨酸残基，因此这两个过程可能会竞争位点，或者它们可能会通过空间位阻或静电效应改变附近位点的底物特异性。O-GlcNAc转移酶已从大鼠肝脏中纯化出来。它以异源三聚体复合物的形式存在，具有两个相同分子量的亚基和一个较短的亚基。这两种多肽是相关的；短亚基带要么是多肽的蛋白水解产物，要么是替代翻译起始位点的产物。O-GlcNAc转移酶以多种转录本的形式表达，这些转录本在不同的人体组织中以不同的数量存在，其中在胰腺中的表达水平最高。表达大鼠O-GlcNAc转移酶的人类细胞的免疫荧光表明，它存在于细胞核和细胞质中。表达O-GlcNAc转移酶的HeLa细胞在长时间孵育过程中存活率不佳，这表明这种蛋白质可能对细胞有毒。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

O-GlcNAc transferase 抗体 (F-12) 参考文献:

  1. O-GlcNAc转移酶基因位于X染色体上，对胚胎干细胞的活力和小鼠的本体发育至关重要。  |  Shafi, R., et al. 2000. Proc Natl Acad Sci U S A. 97: 5735-9. PMID: 10801981
  2. O-GlcNAc转移酶和O-GlcNAc修饰蛋白在大鼠小脑皮质中的定位。  |  Akimoto, Y., et al. 2003. Brain Res. 966: 194-205. PMID: 12618343
  3. 核蛋白质和细胞质蛋白质的糖基化。尿苷二磷酸-N-乙酰葡糖胺：多肽 beta-N-乙酰葡糖胺基转移酶的纯化和表征。  |  Haltiwanger, RS., et al. 1992. J Biol Chem. 267: 9005-13. PMID: 1533623
  4. 确定参与梅花痘病毒感染的秘密因子为 O-GlcNAc 转移酶。  |  Chen, D., et al. 2005. J Virol. 79: 9381-7. PMID: 16014901
  5. O-GlcNAc 转移酶高通量测定法可检测肽底物的主序列偏好。  |  Leavy, TM. and Bertozzi, CR. 2007. Bioorg Med Chem Lett. 17: 3851-4. PMID: 17531489
  6. 磷酸肌醇信号将 O-GlcNAc 转移酶与胰岛素抵抗联系起来。  |  Yang, X., et al. 2008. Nature. 451: 964-9. PMID: 18288188
  7. 蛋白质 O-GlcNAc 糖基化的化学探测进展：结构作用和分子机制》。  |  Saha, A., et al. 2021. Chem Soc Rev. 50: 10451-10485. PMID: 34338261
  8. O-GlcNAc转移酶通过靶向FAM134B介导的ER吞噬调节椎间盘退化。  |  Luo, R., et al. 2022. Exp Mol Med. 54: 1472-1485. PMID: 36056188
  9. 星形胶质细胞中的O-GlcNAc转移酶通过谷氨酸能突触传递调节抑郁症相关的应激敏感性。  |  Fan, J., et al. 2023. J Clin Invest. 133: PMID: 36757814
  10. 肝细胞特异性O-GlcNAc转移酶下调通过改善线粒体功能改善非酒精性脂肪性肝炎。  |  Gonzalez-Rellan, MJ., et al. 2023. Mol Metab. 75: 101776. PMID: 37453647
  11. 核蛋白质和细胞膜蛋白质的动态糖基化。具有多个四肽重复序列的独特 O-GlcNAc 转移酶的克隆和特征描述。  |  Kreppel, LK., et al. 1997. J Biol Chem. 272: 9308-15. PMID: 9083067
  12. O-Linked GlcNAc 转移酶是一种保守的核细胞质蛋白，含有四重肽重复序列。  |  Lubas, WA., et al. 1997. J Biol Chem. 272: 9316-24. PMID: 9083068

订购信息

产品名称产品编号规格价格数量收藏夹

O-GlcNAc transferase 抗体 (F-12)

sc-74546
200 µg/ml
RMB2377.00

O-GlcNAc transferase (F-12): m-IgG Fc BP-HRP 套装

sc-525728
200 µg Ab; 10 µg BP
RMB2662.00

O-GlcNAc transferase (F-12): m-IgG2a BP-HRP 套装

sc-546384
200 µg Ab; 10 µg BP
RMB2662.00

O-GlcNAc transferase 抗体 (F-12) AC

sc-74546 AC
500 µg/ml, 25% agarose
RMB3129.00

O-GlcNAc transferase 抗体 (F-12) HRP

sc-74546 HRP
200 µg/ml
RMB2377.00

O-GlcNAc transferase 抗体 (F-12) FITC

sc-74546 FITC
200 µg/ml
RMB2482.00

O-GlcNAc transferase 抗体 (F-12) PE

sc-74546 PE
200 µg/ml
RMB2580.00

O-GlcNAc transferase 抗体 (F-12) Alexa Fluor® 488

sc-74546 AF488
200 µg/ml
RMB2685.00

O-GlcNAc transferase 抗体 (F-12) Alexa Fluor® 546

sc-74546 AF546
200 µg/ml
RMB2685.00

O-GlcNAc transferase 抗体 (F-12) Alexa Fluor® 594

sc-74546 AF594
200 µg/ml
RMB2685.00

O-GlcNAc transferase 抗体 (F-12) Alexa Fluor® 647

sc-74546 AF647
200 µg/ml
RMB2685.00

O-GlcNAc transferase 抗体 (F-12) Alexa Fluor® 680

sc-74546 AF680
200 µg/ml
RMB2685.00

O-GlcNAc transferase 抗体 (F-12) Alexa Fluor® 790

sc-74546 AF790
200 µg/ml
RMB2685.00

Can you recommend a positive control to use with O-GlcNAc transferase (F-12): sc-74546 monoclonal antibody for Western blot?

Asked by: jenni
We recommend SK-N-MC nuclear extract (sc-2154), A549 (sc-2413) or c4 (sc-364186) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of O-GlcNAc transferase in these cell lines.
Answered by: Technical Support 9
Date published: 2017-01-17
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Rated 4 out of 5 by from Good signal in ImmunofluorescenceWe used this Antibody in Immunosfluorescence (IF) of single mouse zygotes at a 1:200 dilution and we detected good signal compared to a secondary antibody only control. Specificilty cannot be evaluated by this experiment because IF does not give information about size and we did not have an Ogt-KO control sample.
Date published: 2021-11-29
Rated 4 out of 5 by from Great antibodiesI got a free sample from company. Antibodies work great with a high specifity in dilution 1: 1000 (WB)
Date published: 2018-06-10
Rated 4 out of 5 by from Good antibodiesI used this antibody 1:1000 on Western blot with30 ug of protein from endometrial cell lines HEC-1A and Ishikawa . Antibody specifity is high.
Date published: 2018-04-04
Rated 5 out of 5 by from Great!The band was clear at the predicted M.W on the WB (1:1000 dilution, 20 ug C57 mouse myocardium).
Date published: 2017-11-08
Rated 5 out of 5 by from Good o-GlcNAc transferase antibody for WBVery clean and strong WB results with o-GlcNAc transferase antibody .
Date published: 2017-10-18
Rated 5 out of 5 by from The antibody work well in Western blotWestern blot analysis of HAT1 expression in GBC-SD (A), SGC-996 (B), HepG2 (C) whole cell lysates.
Date published: 2017-09-22
Rated 4 out of 5 by from Works for WB in human ES cellsFaint but specific signal with 25 ug of WCL from human ES cells. Used at 1:1000
Date published: 2017-09-15
Rated 4 out of 5 by from a good antibody for WBThis antibody recognizes a band of good signal at the predicted M.W on the WB (1:500 dilution, 40 ug HeLa lysate). I
Date published: 2017-07-30
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O-GlcNAc transferase 抗体 (F-12) is rated 4.4 out of 5 by 16.
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