Date published: 2025-9-7
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NuMA Antibody (F-11) - Western Blotting - Image 403374
NuMA 抗体 (F-11) Alexa Fluor® 488: sc-365532 AF488. 直接荧光西部印迹分析 MCF7 (A) 和 Jurkat (B) 核提取物中 NuMA 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214.
NuMA 抗体 (F-11) Alexa Fluor® 488: sc-365532 AF488. 直接荧光西部印迹分析 MCF7 (A) 和 Jurkat (B) 核提取物中 NuMA 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214.

NuMA 抗体 (F-11): sc-365532

4.5(6)
寫評論提問

说明书
  • NuMA 抗体 F-11 是小鼠单克隆 IgG1 κ，NuMA抗体, 在8篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1816-2115位于human物种的NuMA的C-terminus
  • NuMA 抗体 (F-11) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 NuMA
  • 抗NuMA抗体（F-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NuMA (F-11): sc-365532的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是NuMA Antibody (F-11) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与NuMA Antibody (F-11) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

NuMA抗体（F-11）是一种IgG1κ小鼠单克隆NuMA抗体（也称为核有丝分裂器蛋白1抗体、核基质蛋白-22抗体、SP-H抗原抗体、NMP-22抗体、NuMA抗体、嗜中性粒细胞稳定有丝分裂细胞中的有丝分裂纺锤体抗体、核有丝裂器蛋白抗体、结构核蛋白抗体或NMP22抗体），通过WB、IP、IF、IHC（P）和ELISA检测人源性NuMA蛋白。NuMA抗体（F-11）既有非偶联的抗NuMA抗体形式，也有多种偶联形式的抗NuMA抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞核中有许多结构成分，它们在细胞核组装和有丝分裂方面维持着适当的结构和功能关系。人类核有丝分裂器蛋白基因，也称为NuMA，定位于染色体11q13，编码一种非中心体蛋白。NuMA通过其羧基末端区域具有微管（MT）结合能力，并参与纺锤极组织。从早期有丝分裂到至少后期开始，NuMA对纺锤极的组织和稳定至关重要。在间期，NuMA存在于整个细胞核中，并在进入有丝分裂时定位于纺锤体。在有丝分裂过程中，NuMA形成与微管和某些运动蛋白相互作用的聚集体，因此可能将微管的负端聚集在一起，从而帮助将它们组织成双极纺锤体。与有丝分裂细胞相反，有丝分裂后的神经元在细胞核和细胞质中都显示出NuMA。据报道，癌症患者的NuMA表达水平升高，特别是结直肠癌和早期结直肠癌。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NuMA 抗体 (F-11) 参考文献:

  1. 良性和恶性疾病中的核有丝分裂装置蛋白（NuMA）。  |  Hasholzner, U., et al. 1999. Anticancer Res. 19: 2415-20. PMID: 10470168
  2. 核/有丝分裂器蛋白 NuMA 是神经元体支微管阵列的组成部分。  |  Ferhat, L., et al. 1998. J Neurocytol. 27: 887-99. PMID: 10659681
  3. NuMA：一种参与有丝分裂中心体功能的核蛋白。  |  Zeng, C. 2000. Microsc Res Tech. 49: 467-77. PMID: 10842374
  4. 有丝分裂过程中的染色体移动需要纺锤体两极的微管固定。  |  Gordon, MB., et al. 2001. J Cell Biol. 152: 425-34. PMID: 11157972
  5. NuMA 影响人类乳腺上皮细胞的高阶染色质组织。  |  Abad, PC., et al. 2007. Mol Biol Cell. 18: 348-61. PMID: 17108325
  6. 上皮性卵巢癌中的 NuMA 过表达。  |  Brüning-Richardson, A., et al. 2012. PLoS One. 7: e38945. PMID: 22719996
  7. 在有丝分裂过程中，NuMA 将动力蛋白的活性吸引到微管负端。  |  Hueschen, CL., et al. 2017. Elife. 6: PMID: 29185983
  8. Dynein-Dynactin-NuMA簇作为多臂集合体产生皮质纺锤体拉力。  |  Okumura, M., et al. 2018. Elife. 7: PMID: 29848445
  9. NuMA 集合体组织微管星群，在顶体人类细胞中建立纺锤体双极性。  |  Chinen, T., et al. 2020. EMBO J. 39: e102378. PMID: 31782546
  10. 与有丝分裂装置极区结合的人类特异性核蛋白：在人/鼠杂交细胞中的分布。  |  Lydersen, BK. and Pettijohn, DE. 1980. Cell. 22: 489-99. PMID: 7004645
  11. NuMA，一种参与有丝分裂和核重组的核蛋白。  |  Compton, DA. and Cleveland, DW. 1994. Curr Opin Cell Biol. 6: 343-6. PMID: 7917323
  12. 将核有丝分裂装置蛋白 NuMA 基因归入人类 11q13 染色体。  |  Sparks, CA., et al. 1993. Genomics. 17: 222-4. PMID: 8406455

订购信息

产品名称产品编号规格价格数量收藏夹

NuMA 抗体 (F-11)

sc-365532
200 µg/ml
RMB2377.00

NuMA (F-11): m-IgGκ BP-HRP 套装

sc-522268
200 µg Ab, 40 µg BP
RMB2662.00

NuMA 抗体 (F-11) AC

sc-365532 AC
500 µg/ml, 25% agarose
RMB3129.00

NuMA 抗体 (F-11) HRP

sc-365532 HRP
200 µg/ml
RMB2377.00

NuMA 抗体 (F-11) FITC

sc-365532 FITC
200 µg/ml
RMB2482.00

NuMA 抗体 (F-11) PE

sc-365532 PE
200 µg/ml
RMB2580.00

NuMA 抗体 (F-11) Alexa Fluor® 488

sc-365532 AF488
200 µg/ml
RMB2685.00

NuMA 抗体 (F-11) Alexa Fluor® 546

sc-365532 AF546
200 µg/ml
RMB2685.00

NuMA 抗体 (F-11) Alexa Fluor® 594

sc-365532 AF594
200 µg/ml
RMB2685.00

NuMA 抗体 (F-11) Alexa Fluor® 647

sc-365532 AF647
200 µg/ml
RMB2685.00

NuMA 抗体 (F-11) Alexa Fluor® 680

sc-365532 AF680
200 µg/ml
RMB2685.00

NuMA 抗体 (F-11) Alexa Fluor® 790

sc-365532 AF790
200 µg/ml
RMB2685.00

what is the isoelectric point of NUMA(F-11)

Asked by: htting
Thank you for your question. We do not characterize the isoelectric point for any of our antibodies. Although we do not characterize the isoelectric point of our antibodies, we can find some theoretical information regarding isoelectric point. The isoelectric point (pI) of IgG is found in the range of 6.4 to 9.0 [1]. References: [1] Li G, Stewart R, Conlan B, Gilbert A, Roerth P, Nair H. Purification of human immunoglobulin G: A new approach to plasma fractionation. Vox Sanguinis (2002) 83, 332-338.
Answered by: Technical Support
Date published: 2019-02-22

What is the recommended fixation method for immunofluorescence staining with NuMA (F-11): sc-365532 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-09-29
  • y_2025, m_9, d_7, h_4CST
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Rated 5 out of 5 by from the antibody worked well at 1/500the antibody worked well at 1/500 by IF and PFA fixed cells.
Date published: 2017-11-13
Rated 5 out of 5 by from Great stainingRequested a trial of this antibody for immunofluorescent staining purposes. Wanted to detect mitotic vs. interphase cells and be able to determine spindle orientation. Works fabulously.
Date published: 2017-10-27
Rated 3 out of 5 by from Numa antibodyThis antibody gave a clean but faint signal by western-blot on 60µg of 293T cells when incubated O/N at 4}© at 1/250 dilution in TBST + 10%SVF. Seems specific but not very strong.
Date published: 2017-09-27
Rated 4 out of 5 by from Works for IFI used this for IF staining of brain sections and it works well
Date published: 2017-08-15
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Great Western Blot data of NuMA expression in MOLTGreat Western Blot data of NuMA expression in MOLT-4 nuclear extract. -SCBT QC
Date published: 2014-06-23
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NuMA 抗体 (F-11) is rated 4.5 out of 5 by 6.
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