Date published: 2025-9-11
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NQO1 Antibody (A180) - Western Blotting - Image 376095
NQO1 抗体 (A180): sc-32793. Hep G2 (A), A549 (B), AN3 CA (C) 和 SK-MEL-24 (D) 全细胞裂解液中 NQO1 表达的 Western 印迹分析.
NQO1 抗体 (A180): sc-32793. Hep G2 (A), A549 (B), AN3 CA (C) 和 SK-MEL-24 (D) 全细胞裂解液中 NQO1 表达的 Western 印迹分析.

NQO1 抗体 (A180): sc-32793

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说明书
  • NQO1 抗体 A180 是小鼠单克隆 IgG1 κ，NQO1抗体, 在208篇文献中引用，规格为200 µg/ml
  • 针对human来源的纯化重组NQO1蛋白
  • NQO1 抗体 (A180) 推荐用于WB, IP, IF 和 IHC(P)，检测human, mouse， 以及在较小程度上，检测 rat 来源的 NQO1 ; 不会与 purified human NQO2产生交联反应
  • 抗NQO1抗体（A180）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NQO1 (A180): sc-32793的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 NQO1 抗体 (A180) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NQO1 抗体 (A180) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 NQO 抗体 包括 NQO2

NQO1抗体（A180）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测人类、小鼠和少量大鼠来源的NQO1蛋白。NQO1 (A180) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。NQO1，也称为NAD(P)H：醌氧化还原酶1，通过催化醌还原为氢醌，从而解毒有害化合物并保护细胞免受氧化应激、细胞毒性和诱变性的影响，在细胞防御中起着至关重要的作用。这种酶活性在癌症中尤为重要，因为许多肿瘤会过度表达NQO1，它是一种必需的两电子还原酶，不仅能使毒素失活，还能激活生物还原抗癌药物。NQO1是一种由274个氨基酸组成的蛋白质，在各种组织中普遍表达，但表达水平可能会有很大差异。NQO1基因的表达受到严格控制，可由异生物、抗氧化剂、重金属和辐射等因素诱导，NQO1启动子中的抗氧化反应元件（ARE）在此过程中起着关键作用。叔丁基对苯二酚等化合物激活ARE的过程取决于PI3激酶通路，该通路位于转录因子Nrf2的上游。包括Nrf2、c-Jun、Nrf1、Jun-B和Jun-D在内的各种转录因子与ARE结合，以调节NQO1的表达，而Maf-Maf同源二聚体和Maf-Nrf2异源二聚体可能会对这种转录激活产生负向调节。这种对NQO1的复杂调控对于维持细胞内稳态和应对氧化应激至关重要，这使得抗NQO1抗体（A180）成为研究人员研究这些关键生物学过程的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NQO1 抗体 (A180) 参考文献:

  1. 小 maf（MafG 和 MafK）蛋白负向调节抗氧化反应元件介导的 NAD(P)H:Quinone 氧化还原酶 1 基因的表达和抗氧化诱导。  |  Dhakshinamoorthy, S. and Jaiswal, AK. 2000. J Biol Chem. 275: 40134-41. PMID: 11013233
  2. 编码 NAD(P)H:quinone 氧化还原酶基因的调控。  |  Jaiswal, AK. 2000. Free Radic Biol Med. 29: 254-62. PMID: 11035254
  3. DT-二磷酸酶（NQO1）和 NRH：醌氧化还原酶（NQO2）的结构功能研究。  |  Chen, S., et al. 2000. Free Radic Biol Med. 29: 276-84. PMID: 11035256
  4. 建立 NQO1 基因表达的同源人类结肠肿瘤模型：应用于研究 DT-二磷酸酶在体外和体内生物还原药物活化中的作用。  |  Sharp, SY., et al. 2000. Mol Pharmacol. 58: 1146-55. PMID: 11040064
  5. NAD(P)H：醌氧化还原酶 1 缺乏会增加苯并芘诱发小鼠皮肤癌的易感性。  |  Long, DJ., et al. 2000. Cancer Res. 60: 5913-5. PMID: 11085502
  6. 磷脂酰肌醇 3- 激酶而非细胞外信号调节激酶调节 IMR-32 人类神经母细胞瘤细胞中抗氧化反应元件的激活。  |  Lee, JM., et al. 2001. J Biol Chem. 276: 20011-6. PMID: 11274155
  7. 黄酮醇槲皮素对人类 NAD(P)H:quinone 氧化还原酶 (NQO1) 基因表达的诱导。  |  Valerio, LG., et al. 2001. Toxicol Lett. 119: 49-57. PMID: 11275421
  8. NQO1抑制剂双香豆素通过促进HBx降解来阻断cccDNA的转录。  |  Cheng, ST., et al. 2021. J Hepatol. 74: 522-534. PMID: 32987030
  9. NQO1的多种功能及其在氧化还原控制中的作用。  |  Ross, D. and Siegel, D. 2021. Redox Biol. 41: 101950. PMID: 33774477
  10. NQO1在卵巢癌中的作用。  |  Tossetta, G., et al. 2023. Int J Mol Sci. 24: PMID: 37175546
  11. NQO1失调对中枢神经系统疾病的影响。  |  Yuhan, L., et al. 2024. J Transl Med. 22: 4. PMID: 38167027
  12. NQO1触发中性粒细胞募集和NET形成，推动浸润性乳腺癌的肺转移  |  Wang, X., et al. 2024. Cancer Res. 84: 3538-3555. PMID: 39073320

订购信息

产品名称产品编号规格价格数量收藏夹

NQO1 抗体 (A180)

sc-32793
200 µg/ml
RMB2377.00

NQO1 (A180): m-IgG Fc BP-HRP 套装

sc-528475
200 µg Ab; 10 µg BP
RMB2662.00

NQO1 (A180): m-IgGκ BP-HRP 套装

sc-520864
200 µg Ab, 40 µg BP
RMB2662.00

NQO1 (A180): m-IgG1 BP-HRP 套装

sc-542953
200 µg Ab; 20 µg BP
RMB2662.00

NQO1 抗体 (A180) AC

sc-32793 AC
500 µg/ml, 25% agarose
RMB3129.00

NQO1 抗体 (A180) HRP

sc-32793 HRP
200 µg/ml
RMB2377.00

NQO1 抗体 (A180) FITC

sc-32793 FITC
200 µg/ml
RMB2482.00

NQO1 抗体 (A180) PE

sc-32793 PE
200 µg/ml
RMB2580.00

NQO1 抗体 (A180) Alexa Fluor® 488

sc-32793 AF488
200 µg/ml
RMB2685.00

NQO1 抗体 (A180) Alexa Fluor® 546

sc-32793 AF546
200 µg/ml
RMB2685.00

NQO1 抗体 (A180) Alexa Fluor® 594

sc-32793 AF594
200 µg/ml
RMB2685.00

NQO1 抗体 (A180) Alexa Fluor® 647

sc-32793 AF647
200 µg/ml
RMB2685.00

NQO1 抗体 (A180) Alexa Fluor® 680

sc-32793 AF680
200 µg/ml
RMB2685.00

NQO1 抗体 (A180) Alexa Fluor® 790

sc-32793 AF790
200 µg/ml
RMB2685.00

Dear team, My advisor buy this code NQO1 (A180): sc-32793 in IHC-P application . I would like to ask in Positive control for tonsil Where NQO1 will it be attach at squamous epithelium or vessel ?

Asked by: Yanisorn
Thank you for your question. NQO1 is expressed in the cytoplasm, so cytoplasmic staining is expected. I do not have any information regarding specific attachment in tonsil, so I advice you to look in the primary literature for further information. https://www.uniprot.org/uniprot/P15559
Answered by: Tech Service
Date published: 2019-12-11

We tested the NQO1 A180 antibody on rat spinal cord protein extract and see no band at 45 ug protein. The same sample showed strong band of HO-1 as well as Iba-1. Can you help us to resolve our problem? What kind of secondary antibody should I use?

Asked by: Sungreace
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody. We are happy to help!
Answered by: Tech Service
Date published: 2019-06-24

I want to perform a double stain in my samples, how can I do it with only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using conjugated primary antibodies. This product, sc-32793, is available directly conjugated to FITC, AF488, and AF 647.
Answered by: TechService7
Date published: 2016-12-30
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Rated 5 out of 5 by from GOODthis antibody is very good! we have great result for WB.
Date published: 2024-07-29
Rated 5 out of 5 by from GreatI use this antobody for Western Blot. I get a positive result. I would recommend this great antibody to my colleagues.
Date published: 2023-11-20
Rated 5 out of 5 by from NQO1 antibodyThis antibody has great result for western blot. The band is clean and clear on HaCa T cell.
Date published: 2023-08-06
Rated 2 out of 5 by from NQO1 Slight signal for WBIt showed a slight signal at the expected molecular weight. It shows a more evident nonspecific signal at 55 kDa. I use it at a dilution of 1:1000 on lisates from murine hepatocytes. It seems not so well for my WB.
Date published: 2023-02-17
Rated 5 out of 5 by from Nice antibody!this antibody has great result for western blot analysis!
Date published: 2022-06-20
Rated 5 out of 5 by from very goodvery good for detection of NQO1 in different cell lysates
Date published: 2022-01-24
Rated 5 out of 5 by from excellent！This antibody has great result for western blot analysis
Date published: 2022-01-05
Rated 5 out of 5 by from Great antibody for WBThis primary antibody worked very well in Western blot.
Date published: 2021-03-31
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NQO1 抗体 (A180) is rated 4.8 out of 5 by 34.
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