Date published: 2025-9-9

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NQO1 抗体 (H-9): sc-376023

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说明书
  • NQO1抗体(H-9)是小鼠单克隆IgG2b κ, 在53篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的NQO1的内部区域内的氨基酸201-239之间
  • 抗-NQO1 抗体 (H-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 NQO1; 也和以下物种反应,包括: porcine
  • 抗NQO1抗体(H-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NQO1 (H-9): sc-376023免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 NQO1 抗体 (H-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NQO1 抗体 (H-9) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

NQO1抗体(H-9)是一种IgG2b κ小鼠单克隆NQO1抗体,能够通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的NQO1。NQO1抗体(H-9)既有非偶联抗NQO1抗体的形式,也有多种偶联形式的抗NQO1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。NAD(P)H:醌氧化还原酶1(NQO1)和NRH:醌氧化还原酶(NQO2)是黄素蛋白,它们使用NADH或NADPH作为电子供体,催化醌及其衍生物代谢解毒为氢醌。这可以保护细胞免受醌诱导的氧化应激、细胞毒性和诱变性。许多肿瘤会过度表达NQO1,这是一种必需的两电子还原酶,可使毒素失活并激活生物还原性抗癌药物。NQO1是一种由274个氨基酸组成的蛋白质,在生物体内广泛表达,但其表达水平因组织而异。NQO1基因的表达会对异源物质、抗氧化剂、重金属和辐射产生协调诱导反应。NQO1基因启动子中的抗氧化反应元件(ARE)对于NQO1的表达和协调诱导至关重要。叔丁基氢醌对ARE的激活依赖于PI3激酶,该激酶位于Nrf2的上游。Nrf2、c-Jun、Nrf1、Jun-B和Jun-D与ARE结合,调节NQO1基因的表达和诱导。Maf-Maf同源二聚体和可能的Maf-Nrf2异源二聚体在ARE介导的转录的负调控中发挥作用,但Maf-Nrf1异源二聚体无法与NQO1基因的ARE结合,因此不抑制NQO1的转录。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

NQO1 抗体 (H-9)

sc-376023
200 µg/ml
RMB2377.00

NQO1 (H-9): m-IgGκ BP-HRP 套装

sc-522821
200 µg Ab, 40 µg BP
RMB2662.00

NQO1 (H-9): m-IgG2b BP-HRP 套装

sc-549073
200 µg Ab; 10 µg BP
RMB2662.00

NQO1 抗体 (H-9) AC

sc-376023 AC
500 µg/ml, 25% agarose
RMB3129.00

NQO1 抗体 (H-9) HRP

sc-376023 HRP
200 µg/ml
RMB2377.00

NQO1 抗体 (H-9) FITC

sc-376023 FITC
200 µg/ml
RMB2482.00

NQO1 抗体 (H-9) PE

sc-376023 PE
200 µg/ml
RMB2580.00

NQO1 抗体 (H-9) Alexa Fluor® 488

sc-376023 AF488
200 µg/ml
RMB2685.00

NQO1 抗体 (H-9) Alexa Fluor® 546

sc-376023 AF546
200 µg/ml
RMB2685.00

NQO1 抗体 (H-9) Alexa Fluor® 594

sc-376023 AF594
200 µg/ml
RMB2685.00

NQO1 抗体 (H-9) Alexa Fluor® 647

sc-376023 AF647
200 µg/ml
RMB2685.00

NQO1 抗体 (H-9) Alexa Fluor® 680

sc-376023 AF680
200 µg/ml
RMB2685.00

NQO1 抗体 (H-9) Alexa Fluor® 790

sc-376023 AF790
200 µg/ml
RMB2685.00

NQO1 (H-9) 中和勝肽

sc-376023 P
100 µg/0.5 ml
RMB511.00

How do I use the blocking peptide sc-376023 P? Can I load it on a gel as a positive control?

Asked by: TinTin
The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. It is not recommended to be loaded on the gel as positive control as it is very small. For full protocol details on using the blocking peptide, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 5
Date published: 2017-02-03
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Rated 5 out of 5 by from Excellent antibody!!!i got this antibody and i tried in wb analysis in mouse tumor tissues. Very strong and clear signal.!!
Date published: 2018-05-11
Rated 5 out of 5 by from clear bandStrong and specific signal detected NQO1by WB in human samples
Date published: 2017-04-30
Rated 5 out of 5 by from Great detectionGreat data in WB, IF, IHC(P) for NQO1 detection in multiple cell lines and tissue types. Great antibody.
Date published: 2017-02-07
Rated 5 out of 5 by from Published in WB with MCFPublished in WB with MCF-7 samples. -SCBT QC
Date published: 2015-06-18
Rated 5 out of 5 by from WB: Single strong band in HepG2 samplesWB: Single strong band in HepG2 samples. -SCBT QC
Date published: 2015-05-20
Rated 5 out of 5 by from WB: Single strong band using HeLaWB: Single strong band using HeLa. -SCBT QC
Date published: 2015-05-14
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic and nuclearSuperb immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Great IF images strong cytoplasmic stainingGreat IF images strong cytoplasmic staining in HeLa samples. -SCBT QC
Date published: 2015-04-07
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NQO1 抗体 (H-9) is rated 5.0 out of 5 by 8.
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