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Nociceptin 抗体 (F-10): sc-398073

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说明书
  • Nociceptin 抗体 F-10 是小鼠单克隆 IgG1 (kappa轻链),Nociceptin抗体,规格为200 µg/ml
  • 免疫human物种的代表全长Nociceptin的氨基酸1-176
  • Nociceptin 抗体 (F-10) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 Nociceptin and Neuropeptide1 and Neuropeptide2 processed active peptides
  • 抗Nociceptin抗体(F-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nociceptin (F-10): sc-398073免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Nociceptin Antibody (F-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Nociceptin Antibody (F-10) 打包提供(请参阅下面的订购信息)。

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関連項目

Nociceptin抗体(F-10)是一种IgG1 κ小鼠单克隆Nociceptin抗体(也称为Nociceptin抗体),能够通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验检测人类来源的Nociceptin蛋白。Nociceptin抗体(F-10)既有非偶联型抗Nociceptin抗体形式,也有多种偶联形式的抗Nociceptin抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。伤害感受是一种疼痛反应机制,是机体在受到威胁其完整性的刺激时产生的。由于伤害感受的启动而产生的第一突触受到兴奋性氨基酸(谷氨酸和天冬氨酸)和多种肽类(P物质、CGRP、CCK、内源性阿片肽)的调节。Nociceptin(也称为孤儿素FQ)是一种神经元肽,与阿片肽类似。Nociceptin激活KOR-3(κ型阿片受体,也称为ORL1),这是一种G蛋白偶联受体。尽管与κ型阿片肽--强啡肽A相似,但Nociceptin的作用是使动物对伤害感受刺激产生过度反应。Nociceptin还参与运动行为,并可能参与学习和记忆中的突触可塑性调节。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nociceptin 抗体 (F-10) 参考文献:

  1. 外源性而非内源性痛觉素能调节小鼠间叶多巴胺的释放。  |  Koizumi, M., et al. 2004. J Neurochem. 89: 257-63. PMID: 15030410
  2. 阻断大鼠黑质的痛觉素/表皮素 FQ 传导可逆转氟哌啶醇诱导的运动障碍,并使黑质谷氨酸释放正常化。  |  Marti, M., et al. 2004. J Neurochem. 91: 1501-4. PMID: 15584926
  3. 从核磁共振结构和生物活性关系得出的阿片受体结合的神经肽药理位点。  |  Orsini, MJ., et al. 2005. J Biol Chem. 280: 8134-42. PMID: 15596448
  4. Orphanin FQ:一种激活类阿片 G 蛋白偶联受体的神经肽。  |  Reinscheid, RK., et al. 1995. Science. 270: 792-4. PMID: 7481766
  5. 阿片受体样 ORL1 受体内源性激动剂的分离与结构。  |  Meunier, JC., et al. 1995. Nature. 377: 532-5. PMID: 7566152
  6. 痛觉素能刺激小鼠的运动和探索行为。  |  Florin, S., et al. 1996. Eur J Pharmacol. 317: 9-13. PMID: 8982713
  7. Orphanin FQ/nociceptin通过突触后机制抑制大鼠齿状回的突触传递和长期延时。  |  Yu, TP. and Xie, CW. 1998. J Neurophysiol. 80: 1277-84. PMID: 9744938
  8. 痛觉素和代诺啡 A 激活类阿片受体 1 和卡巴阿片受体的不同机制  |  Mollereau, C., et al. 1999. Mol Pharmacol. 55: 324-31. PMID: 9927625

订购信息

产品名称产品编号规格价格数量收藏夹

Nociceptin 抗体 (F-10)

sc-398073
200 µg/ml
RMB2377.00

Nociceptin (F-10): m-IgG Fc BP-HRP 套装

sc-530689
200 µg Ab; 10 µg BP
RMB2662.00

Nociceptin (F-10): m-IgGκ BP-HRP 套装

sc-524211
200 µg Ab, 40 µg BP
RMB2662.00

Nociceptin (F-10): m-IgG1 BP-HRP 套装

sc-544200
200 µg Ab; 20 µg BP
RMB2662.00

Nociceptin 抗体 (F-10) AC

sc-398073 AC
500 µg/ml, 25% agarose
RMB3129.00

Nociceptin 抗体 (F-10) HRP

sc-398073 HRP
200 µg/ml
RMB2377.00

Nociceptin 抗体 (F-10) FITC

sc-398073 FITC
200 µg/ml
RMB2482.00

Nociceptin 抗体 (F-10) PE

sc-398073 PE
200 µg/ml
RMB2580.00

Nociceptin 抗体 (F-10) Alexa Fluor® 488

sc-398073 AF488
200 µg/ml
RMB2685.00

Nociceptin 抗体 (F-10) Alexa Fluor® 546

sc-398073 AF546
200 µg/ml
RMB2685.00

Nociceptin 抗体 (F-10) Alexa Fluor® 594

sc-398073 AF594
200 µg/ml
RMB2685.00

Nociceptin 抗体 (F-10) Alexa Fluor® 647

sc-398073 AF647
200 µg/ml
RMB2685.00

Nociceptin 抗体 (F-10) Alexa Fluor® 680

sc-398073 AF680
200 µg/ml
RMB2685.00

Nociceptin 抗体 (F-10) Alexa Fluor® 790

sc-398073 AF790
200 µg/ml
RMB2685.00

could I get a 10 ul trial sample of this one? Thank you so much, Lieselot

Asked by: lotsliz
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Tech Service
Date published: 2019-01-16

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Nociceptin (F-10): sc-398073?

Asked by: Professor Griffin
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Primate retinaAntibody was tested under standard conditions (Long Y, Bordt AS, Liu WS, Davis EP, Lee SJ, Tseng L, Chuang AZ, Whitaker CM, Massey SC, Sherman MB, Marshak DW. Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART). Peptides. 2016 Oct;84:22-35). The isolated retina of a baboon was fixed for 60 minutes in 4% paraformaldehyde. Tissue was incubated for 9 days in a 1:1,000 solution of primary antibody with detergent and a blocking agent at 4 °C, washed, then incubated in an affinity-purified secondary antibody for 4 days at 4 °C, washed, and mounted in medium containing DAPI. No peptide blocking control was performed. Results: Bright punctate labeling and low background throughout the optic fiber layer, ganglion cell layer, inner plexiform layer, and inner nuclear layer (less).
Date published: 2017-06-01
Rated 5 out of 5 by from Satisfactory Western blot data of NociceptinSatisfactory Western blot data of Nociceptin expression in non-transfected and human Nociceptin transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-10-27
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Nociceptin 抗体 (F-10) is rated 4.5 out of 5 by 2.
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