Date published: 2025-9-6
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NKG2-D Antibody (5C6) - Western Blotting - Image 285095
NKG2-D 抗体 (5C6): sc-53501. K-562 全细胞裂解液中 NKG2-D 表达的 Western 印迹分析.
NKG2-D Antibody (5C6) - Western Blotting - Image 285095
NKG2-D 抗体 (5C6): sc-53501. K-562 全细胞裂解液中 NKG2-D 表达的 Western 印迹分析.
NKG2-D Antibody (5C6) - Flow Cytometry - Image 14466
NKG2-D 抗体 (5C6): sc-53501. 用 NKG2-D 抗体 (5C6) 染色的 TCR α/β+, CD8+ 人外周血白细胞的间接 FCM 分析, 然后用 PE 结合的山羊抗小鼠 IgG: sc-3738. 黑线直方图代表同型对照, 正常小鼠 IgG2a: sc-3878.
NKG2-D 抗体 (5C6): sc-53501. K-562 全细胞裂解液中 NKG2-D 表达的 Western 印迹分析.

NKG2-D 抗体 (5C6): sc-53501

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说明书
  • NKG2-D 抗体 5C6 是小鼠单克隆 IgG2a κ，NKG2-D抗体, 在6篇文献中引用，规格为200 µg/ml
  • 针对human来源NKL细胞
  • NKG2-D 抗体 (5C6) 推荐用于WB, IP 和 FCM，检测human 来源的 NKG2-D
  • 抗NKG2-D抗体（5C6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NKG2-D (5C6): sc-53501的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是NKG2-D Antibody (5C6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与NKG2-D Antibody (5C6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外NKG2抗体，包括NKG2-D

NKG2-D抗体（5C6）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和流式细胞术（FCM）检测人源NKG2-D蛋白。抗NKG2-D抗体（5C6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。NKG2-D蛋白在免疫反应中发挥着关键作用，特别是通过识别肿瘤细胞中通常过度表达的MICA等应激诱导配体，激活自然杀伤（NK）细胞和某些T细胞。这种相互作用对于免疫系统识别和消除恶性细胞的能力至关重要，使NKG2-D成为癌症免疫治疗的重要靶标。NKG2-D在NK细胞、γδ T细胞和CD8+ αβ T细胞上的表达凸显了其在调节免疫监控和细胞应激反应中的重要性。在研究中使用NKG2-D单克隆抗体（5C6）可以深入了解免疫调节机制，以及NKG2-D在癌症治疗中的潜在治疗应用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NKG2-D 抗体 (5C6) 参考文献:

  1. 应激诱导型 MICA 受体 NKG2D 激活 NK 细胞和 T 细胞。  |  Bauer, S., et al. 1999. Science. 285: 727-9. PMID: 10426993
  2. 小鼠 Ly49 类凝集素受体对自然杀伤细胞的抑制作用  |  Salcedo, M. 1999. Curr Top Microbiol Immunol. 244: 97-105. PMID: 10453652
  3. 一种与杀伤细胞抑制受体同源的新型鼠细胞表面糖蛋白的分子克隆。  |  Hayami, K., et al. 1997. J Biol Chem. 272: 7320-7. PMID: 9054430
  4. NK 细胞上凝集素样受体的不同功能  |  Ryan, JC. and Seaman, WE. 1997. Immunol Rev. 155: 79-89. PMID: 9059884
  5. 自然杀伤细胞受体。  |  Long, EO. and Wagtmann, N. 1997. Curr Opin Immunol. 9: 344-50. PMID: 9203417
  6. HLA I 类特异性抑制受体。  |  Moretta, A. and Moretta, L. 1997. Curr Opin Immunol. 9: 694-701. PMID: 9368779
  7. 克隆小鼠 CD94 的同源物扩展了小鼠自然杀伤细胞上的 C 型凝集素家族。  |  Vance, RE., et al. 1997. Eur J Immunol. 27: 3236-41. PMID: 9464811
  8. 巨噬细胞中主要的 SHP-1 结合蛋白, 酪氨酸磷酸化蛋白是 KIR/LIR 家族的成员，也是 SHP-1 的底物。  |  Berg, KL., et al. 1998. Oncogene. 17: 2535-41. PMID: 9824165

订购信息

产品名称产品编号规格价格数量收藏夹

NKG2-D 抗体 (5C6)

sc-53501
200 µg/ml
RMB2377.00

NKG2-D (5C6): m-IgG Fc BP-HRP 套装

sc-525602
200 µg Ab; 10 µg BP
RMB2662.00

NKG2-D (5C6): m-IgG2a BP-HRP 套装

sc-546335
200 µg Ab; 10 µg BP
RMB2662.00

NKG2-D 抗体 (5C6) AC

sc-53501 AC
500 µg/ml, 25% agarose
RMB3129.00

NKG2-D 抗体 (5C6) HRP

sc-53501 HRP
200 µg/ml
RMB2377.00

NKG2-D 抗体 (5C6) FITC

sc-53501 FITC
200 µg/ml
RMB2482.00

NKG2-D 抗体 (5C6) PE

sc-53501 PE
200 µg/ml
RMB2580.00

NKG2-D 抗体 (5C6) Alexa Fluor® 488

sc-53501 AF488
200 µg/ml
RMB2685.00

NKG2-D 抗体 (5C6) Alexa Fluor® 546

sc-53501 AF546
200 µg/ml
RMB2685.00

NKG2-D 抗体 (5C6) Alexa Fluor® 594

sc-53501 AF594
200 µg/ml
RMB2685.00

NKG2-D 抗体 (5C6) Alexa Fluor® 647

sc-53501 AF647
200 µg/ml
RMB2685.00

NKG2-D 抗体 (5C6) Alexa Fluor® 680

sc-53501 AF680
200 µg/ml
RMB2685.00

NKG2-D 抗体 (5C6) Alexa Fluor® 790

sc-53501 AF790
200 µg/ml
RMB2685.00

Questo Ab può essere utilizzato come anticorpo neutralizzante?

Asked by: Rossellina4
Thank you for your question. We have only tested this antibody in protein detection assays and cannot say whether it would have blocking properties.
Answered by: Tech Support Europe
Date published: 2025-02-12

In the data sheet, the anti-NKG2D (5C6) sc-53501 mAb detects one or two proteins by western blotting analysis of total lysate of K562 cells, a myelogenous leukemia devoid of NKG2D expression. Can you provide data demonstrating that sc-53501 works in wb?

Asked by: Margherita Doria
Thank you for your question. We are not aware of any literature indicating that K562 cells do not express NKG2-D, however we will investigate it further based on your comment. We do not have any additional Western blot data available at this time for this mouse monoclonal, NKG2-D Antibody (5C6): sc-53501. However, we do have positive flow cytometry (FCM) data in CD8+ human peripheral blood leukocytes. We are also aware of a publication citing use of this antibody for immunoprecipitation and Western blot, please see PMID: 21816829 for details. In addition, there is another citation using this antibody specifically in K562 cells, please see PMID: 27057443 for details.
Answered by: Technical Support
Date published: 2017-04-13

Can NKG2-D (5C6): sc-53501 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, NKG2-D (5C6): sc-53501 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from okdai primi test fatti sembrerebbe che l'anticorpo funzioni dopo una blocking di 1h al 5% milk, utilizzando l'ab 1:500 in TBS on e il II 1:3000 3%Milk
Date published: 2023-06-19
Rated 4 out of 5 by from Works for flow cytometryThe NKG2-D antibody works nicely for flow cytometry
Date published: 2018-11-01
Rated 4 out of 5 by from IHC publishable data with human neuroblastoma SHIHC publishable data with human neuroblastoma SH-SY5Y cells, astroglial, and microglial cells - SCBT Publication Review
Date published: 2015-05-13
Rated 4 out of 5 by from Good band detected in WB using KGood band detected in WB using K-562 whole cell lysate. -SCBT QC
Date published: 2015-04-08
Rated 4 out of 5 by from Publishable IP and WB data with human primaryPublishable IP and WB data with human primary NK (natural killer) cells - SCBT Publication Review
Date published: 2015-03-05
Rated 4 out of 5 by from Produced wonderful Indirect FCM data for TCR a/b+Produced wonderful Indirect FCM data for TCR a/b+, CD8+ human peripheral blood leukocytes stained with NKG2-D . -SCBT QC
Date published: 2013-06-13
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NKG2-D 抗体 (5C6) is rated 4.0 out of 5 by 6.
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