Date published: 2026-10-24

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NGFR p75 Antibody (B-1) - Western Blotting - Image 135978
NGFR p75 抗体 (B-1): sc-271708. Western 印迹分析 SK-N-NC 全细胞裂解液中 NGFR p75 的表达.
NGFR p75 抗体 (B-1): sc-271708. Western 印迹分析 SK-N-NC 全细胞裂解液中 NGFR p75 的表达.

NGFR p75 抗体 (B-1): sc-271708

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说明书
  • NGFR p75 抗体 B-1 是小鼠单克隆 IgG1 κ，NGFR p75抗体, 在40篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的NGFR p75的C-terminus的氨基酸393-427之间
  • NGFR p75 抗体 (B-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 NGFR p75
  • 抗NGFR p75抗体（B-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是NGFR p75 Antibody (B-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与NGFR p75 Antibody (B-1) 打包提供（请参阅下面的订购信息）。
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NGFR p75抗体（B-1）是一种IgG1 κ小鼠单克隆NGFR p75抗体（也称为NGFR抗体、p75NTR抗体、TNFRSF16抗体或神经生长因子（NGF）受体抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的NGFR p75蛋白。NGFR p75抗体（B-1）既有非偶联形式的抗NGFR p75抗体，也有多种偶联形式的抗NGFR p75抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Trk癌基因编码一种膜跨越蛋白酪氨酸激酶gp140Trk，其表达在体内仅限于来源于神经嵴的感觉脊髓和颅神经节的神经元。神经生长因子（NGF）刺激神经细胞系和胚胎背根神经节中Trk A的酪氨酸磷酸化。NGF对Trk的酪氨酸磷酸化作用迅速、特异，并且发生在皮摩尔量级的因子上，表明该反应是由生理量的NGF介导的，提示Trk A参与了NGF的主要信号转导机制。Trk A受体复合物的另一个组分NGFR p75以低亲和力与神经营养因子结合，但对于有效信号传导是必需的。NGFR p75加速Trk A的激活，并可能将下游效应分子募集到配体复合物中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NGFR p75 抗体 (B-1) 参考文献:

  1. 在生理条件下，p75神经营养因子受体（p75NTR）会改变肿瘤坏死因子介导的NF-κB活性，但直接p75NTR介导的NF-κB激活需要细胞应激。  |  Bhakar, AL., et al. 1999. J Biol Chem. 274: 21443-9. PMID: 10409708
  2. p75神经营养素受体的膜内蛋白水解调节了其与TrkA受体的缔合作用。  |  Jung, KM., et al. 2003. J Biol Chem. 278: 42161-9. PMID: 12913006
  3. 有证据表明，肿瘤坏死因子α转化酶（TACE）在p75神经营养因子受体（p75NTR）的外部结构脱落中起着关键作用。  |  Weskamp, G., et al. 2004. J Biol Chem. 279: 4241-9. PMID: 14638693
  4. p75神经营养因子受体的表达可抵御β-淀粉样蛋白可溶性低聚体的神经毒性。  |  Costantini, C., et al. 2005. Exp Cell Res. 311: 126-34. PMID: 16223482
  5. 神经营养因子p75受体（p75NTR）促进内皮细胞凋亡并抑制血管生成：对糖尿病引起的缺血性肢体肌肉新血管生成受损的影响。  |  Caporali, A., et al. 2008. Circ Res. 103: e15-26. PMID: 18566344
  6. 神经营养因子受体p75NTR在炎症性肌病中诱导成熟肌纤维，并促进肌管在炎症应激中的存活。  |  Colombo, E., et al. 2012. Neuropathol Appl Neurobiol. 38: 367-78. PMID: 21851375
  7. p75神经营养因子受体调节MC3T3-E1成骨细胞分化。  |  Mikami, Y., et al. 2012. Differentiation. 84: 392-9. PMID: 22906707
  8. p75神经营养因子受体在神经细胞中逃避内溶酶体途径，有利于多囊泡体专门用于外泌体释放。  |  Escudero, CA., et al. 2014. J Cell Sci. 127: 1966-79. PMID: 24569882
  9. p75神经营养因子受体的裂解与阿尔茨海默氏病有关。  |  Chao, MV. 2016. Mol Psychiatry. 21: 300-1. PMID: 26782055
  10. p75神经营养因子受体与TRAF6的关联。  |  Khursigara, G., et al. 1999. J Biol Chem. 274: 2597-600. PMID: 9915784

订购信息

产品名称产品编号规格价格数量收藏夹

NGFR p75 抗体 (B-1)

sc-271708
200 µg/ml
CNY2422.00

NGFR p75 (B-1): m-IgG Fc BP-HRP 套装

sc-529246
200 µg Ab; 10 µg BP
CNY2715.00

NGFR p75 (B-1): m-IgGκ BP-HRP 套装

sc-522033
200 µg Ab, 40 µg BP
CNY2715.00

NGFR p75 (B-1): m-IgG1 BP-HRP 套装

sc-543412
200 µg Ab; 20 µg BP
CNY2715.00

NGFR p75 抗体 (B-1) AC

sc-271708 AC
500 µg/ml, 25% agarose
CNY3189.00

NGFR p75 抗体 (B-1) HRP

sc-271708 HRP
200 µg/ml
CNY2422.00

NGFR p75 抗体 (B-1) FITC

sc-271708 FITC
200 µg/ml
CNY2527.00

NGFR p75 抗体 (B-1) PE

sc-271708 PE
200 µg/ml
CNY2625.00

NGFR p75 抗体 (B-1) Alexa Fluor® 488

sc-271708 AF488
200 µg/ml
CNY2738.00

NGFR p75 抗体 (B-1) Alexa Fluor® 546

sc-271708 AF546
200 µg/ml
CNY2738.00

NGFR p75 抗体 (B-1) Alexa Fluor® 594

sc-271708 AF594
200 µg/ml
CNY2738.00

NGFR p75 抗体 (B-1) Alexa Fluor® 647

sc-271708 AF647
200 µg/ml
CNY2738.00

NGFR p75 抗体 (B-1) Alexa Fluor® 680

sc-271708 AF680
200 µg/ml
CNY2738.00

NGFR p75 抗体 (B-1) Alexa Fluor® 790

sc-271708 AF790
200 µg/ml
CNY2738.00

NGFR p75 (B-1) 中和勝肽

sc-271708 P
100 µg/0.5 ml
CNY519.00

Hi, what is the best gel percentage for p75 to avoid having many bands? Also, which blocking solution do you use? BSA or Milk? And in which solution do you prepare the antibody?

Asked by: ka92
Thank you for your question. As the molecular weight of p75 is 75 kDa it is recommended to use a gel percentage of 10%. For blocking and antibody dilution we use UltraCruz® Blocking Reagent (sc-516214)
Answered by: Tech Support Europe
Date published: 2021-07-23

Can this antibody also be used for FACS?

Asked by: Libka
Thank you for your question. Anti-NGFR p75 Antibody (B-1) is available with fluorescent conjugates, which are suited for FACS.
Answered by: Tech Support Europe
Date published: 2021-07-14

Hi! I wonder which is the best method for WB analysis. In particular which blocking solution do you use?

Asked by: vale89
Thank you for your question. We recommend using a blocking buffer of 5% milk TBST for Western Blot. Our complete Western Blot protocol can be found on the website here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Service
Date published: 2017-07-13

What is the recommended fixation method for IF with NGFR p75 (B-1): sc-271708 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 8
Date published: 2017-02-03
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Rated 5 out of 5 by saralo from Great AntibodyI used it in WB at 1:300 ratio and I obtained a strong and specific band.
Date published: 2022-06-06
Rated 5 out of 5 by lining7584 from greatIt works great, the response is perfect, I get the results I want
Date published: 2022-02-03
Rated 5 out of 5 by Ceci00 from NGFR p75 antibody fo IHCI used this antibody for IHC on paraffin sectins. It works very well with a good positivecytoplasmic signal, anyway I recommend also the use of the corresponding blocking peptid, because it is possible to seea background signal.
Date published: 2020-10-02
Rated 4 out of 5 by marzia from For me it's okThe antibody anti-NGFR p75 (B-1; sc-271708) was tested for Western blotting. The samples analyzed derive from human cancer cells known to be positive or negative for the p75 expression. As shown in the western blot, the antibody recognizes two bands: a specific band corresponding to 75 KDa (as expected) and an aspecific band at about 100 KDa (as expected). Western blot conditions: Blocking 5% Milk; Ab I 1:500 in tTBS Ab II 1:2000 anti-mouse in 3% Milk
Date published: 2019-05-31
Rated 4 out of 5 by LBMN from IF on mouse lung works fineImmunofluorescence staining on mouse lung works fine with this ab.
Date published: 2018-09-19
Rated 4 out of 5 by Lelli from Clear staining in western blotI used this antibody on primary brain culture and it works very well in western blot even at low concentrations (1:2000)
Date published: 2018-05-25
Rated 5 out of 5 by Marco84 from Amazing Western blot and IHC resultsI tried several anti-p75NGFR antibodies (also produced by different companies). However, this antibody is became my first choice. We tested sc-271708 on rat brain and human tissues, and it gives a clear and strong signal at 75 kDa, which corresponds to the mature glycosylated form of the receptor. A band at about 60 kDa is also present, corresponding to the immature form of the receptor. Depending on the physiopathological conditions, it is also possible to observe p75 cleavage products.
Date published: 2017-07-14
Rated 5 out of 5 by linghan123 from excellent antibodyI bought this antibody use for pig and rat peripheral nervous tissue cryosections immunofluorescence stain. We got perfect image results.
Date published: 2017-05-19
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NGFR p75 抗体 (B-1) is rated 4.8 out of 5 by 16.
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