Date published: 2025-9-8
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NGFR p75 Antibody (ME20.4) - Western Blotting - Image 53545
NGFR p75 抗体 (ME20. 4): sc-13577. 对 SK-N-MC 全细胞裂解液中 NGFR p75 的表达进行 Western 印迹分析.
NGFR p75 抗体 (ME20. 4): sc-13577. 对 SK-N-MC 全细胞裂解液中 NGFR p75 的表达进行 Western 印迹分析.

NGFR p75 抗体 (ME20.4): sc-13577

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寫評論提問

说明书
  • NGFR p75抗体(ME20.4)是小鼠单克隆IgG1 κ, 在39篇文献中引用，规格为200 µg/ml
  • 针对human来源的WM245黑色素瘤细胞
  • 抗-NGFR p75 抗体 (ME20.4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 NGFR p75; 也和以下物种反应，包括： 和 bovine, porcine, feline and canine
  • 抗NGFR p75抗体（ME20.4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NGFR p75 (ME20.4): sc-13577的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成NGFR p75 抗体 (ME20.4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

NGFR p75抗体（ME20.4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠和人类样本中的NGFR p75。抗NGFR p75抗体（ME20.4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。NGFR p75通过调节神经生长因子（NGF）等神经生长因子的作用，在神经系统发挥关键作用。NGFR p75主要位于神经元的表面，对神经元的存活和分化至关重要。NGFR p75通过与低亲和力的神经营养因子结合，提高高亲和力Trk受体（如Trk A）的信号传导效率，这对于激活下游信号通路至关重要，从而促进神经元的健康和功能。NGFR p75和Trk A之间的相互作用不仅加速了Trk A的激活，还促进了下游效应分子的募集，从而在神经生长因子信号级联中发挥了重要作用。NGFR p75是神经退行性疾病和神经元发育研究的重要靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NGFR p75 抗体 (ME20.4) 参考文献:

  1. NGF 诱导的 trk 原癌基因产物的酪氨酸磷酸化和酪氨酸激酶活性。  |  Kaplan, DR., et al. 1991. Nature. 350: 158-60. PMID: 1706478
  2. trk 原癌基因编码神经生长因子的受体。  |  Klein, R., et al. 1991. Cell. 65: 189-97. PMID: 1849459
  3. 高亲和力 NGF 结合需要 trk 原癌基因和低亲和力 NGF 受体的共同表达。  |  Hempstead, BL., et al. 1991. Nature. 350: 678-83. PMID: 1850821
  4. 候选干细胞标记物p75神经营养因子受体（p75NTR）在循环肿瘤细胞中的表达的临床意义。  |  Okumura, T., et al. 2017. Adv Exp Med Biol. 994: 247-254. PMID: 28560678
  5. 一种由截短的肌球蛋白和蛋白酪氨酸激酶序列融合而成的人类癌基因。  |  Martin-Zanca, D., et al. Nature. 319: 743-8. PMID: 2869410
  6. 人类 trk 原癌基因的分子和生化特征。  |  Martin-Zanca, D., et al. 1989. Mol Cell Biol. 9: 24-33. PMID: 2927393
  7. 人类肌球蛋白基因的组织特异性表达与 trk 致癌基因的产生有关。  |  Reinach, FC. and MacLeod, AR. Nature. 322: 648-50. PMID: 3018581
  8. P75神经营养因子受体作为治疗神经疾病药物开发的治疗靶点。  |  Xiong, LL., et al. 2022. Eur J Neurosci. 56: 5299-5318. PMID: 36017737
  9. 小鼠缺血性中风后，P75神经营养因子受体的遗传和药理调节可减轻脑损伤。  |  Mirzahosseini, G., et al. 2024. Mol Neurobiol. 61: 276-293. PMID: 37606717
  10. 帕金森病中p75神经营养因子受体（p75NTR）的分子通路：新进展。  |  Ali, NH., et al. 2024. Mol Neurobiol. 61: 2469-2480. PMID: 37897634
  11. 小鸡胚胎神经肌肉系统发育过程中 trkB 和 p75 的表达以及 BDNF 的作用。  |  McKay, SE., et al. 1996. Development. 122: 715-24. PMID: 8625822
  12. p75（NGFR）和 TrkA 受体相互协作，迅速激活 p75（NGFR）相关蛋白激酶。  |  Canossa, M., et al. 1996. EMBO J. 15: 3369-76. PMID: 8698038

订购信息

产品名称产品编号规格价格数量收藏夹

NGFR p75 抗体 (ME20.4)

sc-13577
200 µg/ml
RMB2377.00

NGFR p75 抗体 (ME20.4) AC

sc-13577 AC
500 µg/ml, 25% agarose
RMB3129.00

NGFR p75 抗体 (ME20.4) HRP

sc-13577 HRP
200 µg/ml
RMB2377.00

NGFR p75 抗体 (ME20.4) FITC

sc-13577 FITC
200 µg/ml
RMB2482.00

NGFR p75 抗体 (ME20.4) PE

sc-13577 PE
200 µg/ml
RMB2580.00

NGFR p75 抗体 (ME20.4) Alexa Fluor® 488

sc-13577 AF488
200 µg/ml
RMB2685.00

NGFR p75 抗体 (ME20.4) Alexa Fluor® 546

sc-13577 AF546
200 µg/ml
RMB2685.00

NGFR p75 抗体 (ME20.4) Alexa Fluor® 594

sc-13577 AF594
200 µg/ml
RMB2685.00

NGFR p75 抗体 (ME20.4) Alexa Fluor® 647

sc-13577 AF647
200 µg/ml
RMB2685.00

NGFR p75 抗体 (ME20.4) Alexa Fluor® 680

sc-13577 AF680
200 µg/ml
RMB2685.00

NGFR p75 抗体 (ME20.4) Alexa Fluor® 790

sc-13577 AF790
200 µg/ml
RMB2685.00

I want to know if the sc-13577 can bind with human MSCs?

Asked by: ziziyanyan
Thank you for your question. Anti-NGFR p75 Antibody (ME20.4) is recommended for detection of NGFR p75 of mouse, rat and human origin by WB, IP, IF and IHC(P). It should recognize its epitope in all human cells that express it.
Answered by: Tech Support Europe
Date published: 2020-06-17

I want to study p75NTR placental samples. My application will be IHC. Please tell me whether the this antibody suitable for placental samples or not.

Asked by: Prachi
Thank you for your question. We do not have any data or product citations using this NGFR p75 antibody in placental samples.
Answered by: Technical Support
Date published: 2020-05-04

What is the recommended fixation method for immunofluorescence staining with NGFR p75 (ME20.4): sc-13577 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from One laneThe antibody anti-NGFR p75 (ME20.4; sc-13577) was tested by Western blotting analysis. The samples loaded (30 μg total of proteins) derive from human cancer cells known to be positive (ctrl +) or negative (ctrl -) for the p75 expression. As shown in the western blot, the antibody recognizes only a band corresponding to 75 KDa. Despite the high amount of proteins loaded the bands observed are very faint. Probably the primary antibody must be used more concentrated (e.g 1:200) Western blot conditions: Blocking 5% Milk (1h- RT); Ab I 1:500 in tTBS overnight (+4°C) ; Ab II 1:2000 anti-mouse in 3% Milk (1h-RT)
Date published: 2019-05-31
Rated 5 out of 5 by from Methanol fixed IF is GreatIF staining of methanol fixed cells shows excellent membrane localization.
Date published: 2017-01-20
Rated 5 out of 5 by from Published IHC data for use in thymic cellsPublished IHC data for use in thymic cells -SCBT Publication Review
Date published: 2015-05-05
Rated 4 out of 5 by from Published data in IHC using human oral squamousPublished data in IHC using human oral squamous cell carcinoma samples -SCBT Publication Review
Date published: 2015-04-27
Rated 4 out of 5 by from Great immunofluorescence membrane stainingGreat immunofluorescence membrane staining in methanol-fixed Hs 294T cells. -SCBT QC
Date published: 2015-04-05
Rated 4 out of 5 by from Very favorable WB band achieved using SKVery favorable WB band achieved using SK-N-MC whole cell lysate -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Published data in RPE cells using this antibodyPublished data in RPE cells using this antibody for Western blot analysis -SCBT Publication Review
Date published: 2015-01-14
Rated 4 out of 5 by from Nice publishable data on Western blot onNice publishable data on Western blot on human brain tissue. -SCBT Publication Review
Date published: 2015-01-07
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NGFR p75 抗体 (ME20.4) is rated 4.3 out of 5 by 8.
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