Date published: 2025-9-8
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NGFR p75 Antibody (5H1) - Western Blotting - Image 377271
NGFR p75 抗体 (5H1): sc-71693. Neuro-2A (A), F9 (B), NIH/3T3 (C), U-87 MG (D) 和 PC-3 (E) 全细胞裂解液中 NGFR p75 表达的 Western 印迹分析.
NGFR p75 抗体 (5H1): sc-71693. Neuro-2A (A), F9 (B), NIH/3T3 (C), U-87 MG (D) 和 PC-3 (E) 全细胞裂解液中 NGFR p75 表达的 Western 印迹分析.

NGFR p75 抗体 (5H1): sc-71693

4.3(3)
寫評論提問

说明书
  • NGFR p75抗体(5H1)是小鼠单克隆IgG1 κ, 在2篇文献中引用，规格为200 µg/ml
  • 针对human来源的WM245黑色素瘤细胞
  • 推荐用于 mouse, rat 和 human 来源的NGFR p75 WB, IP, IF 和 IHC(P)检测; 也和以下物种反应，包括： 和 bovine, porcine, feline and canine
  • 看 NGFR p75 (B-1): sc-271708，可查看有标记的 NGFR p75 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG1 BP-HRP 是 NGFR p75 抗体 (5H1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与NGFR p75 抗体 (5H1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Trk 癌基因编码跨膜蛋白酪氨酸激酶 gp140Trk，其表达在体内仅限于神经嵴起源的感觉脊髓和颅神经节的神经元。神经生长因子 (NGF) 刺激神经细胞系和胚胎背根神经节中 Trk A 的酪氨酸磷酸化。 NGF 对 Trk A 的酪氨酸磷酸化是快速、特异性的，并且以皮摩尔数量的因子发生，表明该反应是由生理量的 NGF 介导的，表明 Trk A 参与 NGF 的主要信号转导机制。 Trk A 受体复合物的另一个组分 NGFR p75 以低亲和力与神经营养因子结合，但有效信号传导是必需的。 NGFR p75 加速 Trk A 激活，并可能招募下游效应分子到结合复合物。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

NGFR p75 抗体 (5H1)

sc-71693
200 µg/ml
RMB2377.00

NGFR p75 (5H1): m-IgG1 BP-HRP 套装

sc-542618
200 µg Ab; 20 µg BP
RMB2662.00

p75NTR usually runs around 75 kDa with the post-translational modifications but on the 20 samples I had, it ended up running around 95 to 100 kDa. Does anyone knows why?

Asked by: SammyBoi
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-06-15

How much sample do you recommend loading on the gel for Western blot detection using NGFR p75 (5H1): sc-71693?

Asked by: Dr Ninau Qelp
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Published data in uterine tissue using thisPublished data in uterine tissue using this antibody for Western blot analysis -SCBT Publication Review
Date published: 2015-06-19
Rated 4 out of 5 by from Good signal using this antibody against SKGood signal using this antibody against SK-N-MC whole cell lysate in WB. -SCBT QC
Date published: 2015-03-30
Rated 4 out of 5 by from IHC(P) publishable data with human uterine tissueIHC(P) publishable data with human uterine tissue - SCBT Publication Review
Date published: 2015-03-26
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NGFR p75 抗体 (5H1) is rated 4.3 out of 5 by 3.
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