Date published: 2026-10-24

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NG2 抗体 (132.38): sc-33666

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说明书
  • NG2抗体(132.38)是小鼠单克隆IgG1 κ, 在8篇文献中引用,规格为200 µg/ml
  • 针对表达NG2的氨基酸1592-2222的截短整体膜形式的HEK293细胞
  • 抗-NG2 抗体 (132.38) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse 和rat 来源的 NG2
  • 抗NG2抗体(132.38)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是NG2 Antibody (132.38) for WB and IHC(P) applications. 的首选辅助检测试剂,这些试剂现与NG2 Antibody (132.38) 打包提供(请参阅下面的订购信息)。
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NG2抗体(132.38)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学等方法检测小鼠和大鼠样本中的NG2。抗NG2抗体(132.38)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。NG2,也称为黑色素瘤相关硫酸软骨素蛋白多糖4、MCSP、MCSPG、MSK16和MEL-CSPG,在黑色素瘤细胞在血管内皮基底膜上扩散的早期阶段,对稳定细胞与基底膜的相互作用起着至关重要的作用,而这一过程对肿瘤的进展至关重要。NG2主要位于各种细胞类型(包括大鼠胚胎中的血管和中枢神经系统中的内皮细胞)的表面,有助于微血管的结构完整性和功能性。此外,NG2在中枢神经系统外的平滑肌细胞上表达,并作为表皮干细胞的新标记,促进其聚集和模式化分布。值得注意的是,大鼠NG2(人类NG2中的苏氨酸2252)中的苏氨酸2256的磷酸化会导致星形细胞瘤表面的重新分布,从而增强细胞极化和运动性,而这两者是癌症转移的关键因素。NG2还是α4β1整合素的辅助受体,可促进恶性黑色素瘤细胞的扩散和局灶性接触的形成,从而凸显其在癌症生物学和潜在治疗靶向中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NG2 抗体 (132.38) 参考文献:

  1. NG2细胞及其神经生成潜力。  |  Kirdajova, D. and Anderova, M. 2020. Curr Opin Pharmacol. 50: 53-60. PMID: 31877531
  2. 脊髓损伤后,NG2胶质细胞的体内重编程可促进成体神经发生和功能恢复。  |  Tai, W., et al. 2021. Cell Stem Cell. 28: 923-937.e4. PMID: 33675690
  3. NG2胶质细胞释放的GABA可调节抑制性突触,并导致应激性焦虑。  |  Zhang, X., et al. 2021. Nat Commun. 12: 5740. PMID: 34593806
  4. 骨髓NG2+/Nestin+间充质干细胞通过TGFβ2驱动DTC休眠。  |  Nobre, AR., et al. 2021. Nat Cancer. 2: 327-339. PMID: 34993493
  5. 骨转移的启动与NG2+细胞的成骨分化引起的骨重塑有关。  |  Zhang, W., et al. 2023. Cancer Discov. 13: 474-495. PMID: 36287038
  6. NG2胶质细胞中的Kir4.1通道激活有助于缺血性中风中的髓鞘再生。  |  Hong, X., et al. 2023. EBioMedicine. 87: 104406. PMID: 36527899
  7. 皮质NG2-胶质细胞对创伤性脑损伤的反应。  |  Dean, T., et al. 2023. Glia. 71: 1164-1175. PMID: 36692058
  8. 脊髓损伤后,NG2胶质细胞重编程可诱导强大的轴突再生。  |  Tai, W., et al. 2024. iScience. 27: 108895. PMID: 38318363
  9. NG2胶质细胞通过抑制小胶质细胞对神经元的前列腺素E2信号传导,从而防止朊病毒神经毒性。  |  Liu, Y., et al. 2024. Nat Neurosci. 27: 1534-1544. PMID: 38802591
  10. NG2是MLL-AF4的目标基因,通过调节NR3C1的表达,在MLLr B-ALL中起着糖皮质激素抗性的基础作用。  |  Lopez-Millan, B., et al. 2024. Blood. 144: 2002-2017. PMID: 39093982

订购信息

产品名称产品编号规格价格数量收藏夹

NG2 抗体 (132.38)

sc-33666
200 µg/ml
CNY2422.00

NG2 (132.38): m-IgG Fc BP-HRP 套装

sc-526699
200 µg Ab; 10 µg BP
CNY2715.00

NG2 (132.38): m-IgG1 BP-HRP 套装

sc-532072
200 µg Ab; 20 µg BP
CNY2715.00

NG2 抗体 (132.38) AC

sc-33666 AC
500 µg/ml, 25% agarose
CNY3189.00

NG2 抗体 (132.38) HRP

sc-33666 HRP
200 µg/ml
CNY2422.00

NG2 抗体 (132.38) FITC

sc-33666 FITC
200 µg/ml
CNY2527.00

NG2 抗体 (132.38) PE

sc-33666 PE
200 µg/ml
CNY2625.00

NG2 抗体 (132.38) Alexa Fluor® 488

sc-33666 AF488
200 µg/ml
CNY2738.00

NG2 抗体 (132.38) Alexa Fluor® 546

sc-33666 AF546
200 µg/ml
CNY2738.00

NG2 抗体 (132.38) Alexa Fluor® 594

sc-33666 AF594
200 µg/ml
CNY2738.00

NG2 抗体 (132.38) Alexa Fluor® 647

sc-33666 AF647
200 µg/ml
CNY2738.00

NG2 抗体 (132.38) Alexa Fluor® 680

sc-33666 AF680
200 µg/ml
CNY2738.00

NG2 抗体 (132.38) Alexa Fluor® 790

sc-33666 AF790
200 µg/ml
CNY2738.00

How much sample do you recommend loading on the gel for Western blot detection using NG2 (132.38): sc-33666?

Asked by: Professor Griffin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by Bell56 from Worked for WBNG2 is large and I was worried about running the gel out enough to get a good blot, but it all worked out pretty easily with my normal protocol and a 10% gel. Clear band for NG2.
Date published: 2019-01-18
Rated 5 out of 5 by jerojero from Excellent!This antibody works great in western blot analysis of NG2 expression in rat brain and rat thyroid tissue extracts.
Date published: 2017-01-30
Rated 5 out of 5 by JM from Robust signal in WB in rat brain and ratRobust signal in WB in rat brain and rat thyroid tissue extracts. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by TO from IF publishable data with human primary OPCIF publishable data with human primary OPC cultures - SCBT Publication Review
Date published: 2014-09-23
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NG2 抗体 (132.38) is rated 5.0 out of 5 by 4.
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