Date published: 2025-9-7

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NG2 抗体 (132.38): sc-33666

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说明书
  • NG2抗体(132.38)是小鼠单克隆IgG1 κ, 在8篇文献中引用,规格为200 µg/ml
  • 针对表达NG2的氨基酸1592-2222的截短整体膜形式的HEK293细胞
  • 抗-NG2 抗体 (132.38) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse 和rat 来源的 NG2
  • 抗NG2抗体(132.38)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NG2 (132.38): sc-33666免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 NG2 抗体 (132.38) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NG2 抗体 (132.38) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

NG2抗体(132.38)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学等方法检测小鼠和大鼠样本中的NG2。抗NG2抗体(132.38)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。NG2,也称为黑色素瘤相关硫酸软骨素蛋白多糖4、MCSP、MCSPG、MSK16和MEL-CSPG,在黑色素瘤细胞在血管内皮基底膜上扩散的早期阶段,对稳定细胞与基底膜的相互作用起着至关重要的作用,而这一过程对肿瘤的进展至关重要。NG2主要位于各种细胞类型(包括大鼠胚胎中的血管和中枢神经系统中的内皮细胞)的表面,有助于微血管的结构完整性和功能性。此外,NG2在中枢神经系统外的平滑肌细胞上表达,并作为表皮干细胞的新标记,促进其聚集和模式化分布。值得注意的是,大鼠NG2(人类NG2中的苏氨酸2252)中的苏氨酸2256的磷酸化会导致星形细胞瘤表面的重新分布,从而增强细胞极化和运动性,而这两者是癌症转移的关键因素。NG2还是α4β1整合素的辅助受体,可促进恶性黑色素瘤细胞的扩散和局灶性接触的形成,从而凸显其在癌症生物学和潜在治疗靶向中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

NG2 抗体 (132.38)

sc-33666
200 µg/ml
RMB2377.00

NG2 (132.38): m-IgG Fc BP-HRP 套装

sc-526699
200 µg Ab; 10 µg BP
RMB2662.00

NG2 (132.38): m-IgG1 BP-HRP 套装

sc-532072
200 µg Ab; 20 µg BP
RMB2662.00

NG2 抗体 (132.38) AC

sc-33666 AC
500 µg/ml, 25% agarose
RMB3129.00

NG2 抗体 (132.38) HRP

sc-33666 HRP
200 µg/ml
RMB2377.00

NG2 抗体 (132.38) FITC

sc-33666 FITC
200 µg/ml
RMB2482.00

NG2 抗体 (132.38) PE

sc-33666 PE
200 µg/ml
RMB2580.00

NG2 抗体 (132.38) Alexa Fluor® 488

sc-33666 AF488
200 µg/ml
RMB2685.00

NG2 抗体 (132.38) Alexa Fluor® 546

sc-33666 AF546
200 µg/ml
RMB2685.00

NG2 抗体 (132.38) Alexa Fluor® 594

sc-33666 AF594
200 µg/ml
RMB2685.00

NG2 抗体 (132.38) Alexa Fluor® 647

sc-33666 AF647
200 µg/ml
RMB2685.00

NG2 抗体 (132.38) Alexa Fluor® 680

sc-33666 AF680
200 µg/ml
RMB2685.00

NG2 抗体 (132.38) Alexa Fluor® 790

sc-33666 AF790
200 µg/ml
RMB2685.00

How much sample do you recommend loading on the gel for Western blot detection using NG2 (132.38): sc-33666?

Asked by: Professor Griffin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked for WBNG2 is large and I was worried about running the gel out enough to get a good blot, but it all worked out pretty easily with my normal protocol and a 10% gel. Clear band for NG2.
Date published: 2019-01-18
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of NG2 expression in rat brain and rat thyroid tissue extracts.
Date published: 2017-01-30
Rated 5 out of 5 by from Robust signal in WB in rat brain and ratRobust signal in WB in rat brain and rat thyroid tissue extracts. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from IF publishable data with human primary OPCIF publishable data with human primary OPC cultures - SCBT Publication Review
Date published: 2014-09-23
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NG2 抗体 (132.38) is rated 5.0 out of 5 by 4.
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