Date published: 2025-9-8
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NF-1 Antibody (A-12) - Western Blotting - Image 356644
NF-1 抗体 (A-12): sc-74445. MCF7 (A), A-431 (B), L929 (C), Sol8 (D), KNRK (E) 和 L8 (F) 全细胞裂解液中 NF-1 表达的 Western 印迹分析.
NF-1 抗体 (A-12): sc-74445. MCF7 (A), A-431 (B), L929 (C), Sol8 (D), KNRK (E) 和 L8 (F) 全细胞裂解液中 NF-1 表达的 Western 印迹分析.

NF-1 抗体 (A-12): sc-74445

4.3(4)
寫評論提問

说明书
  • NF-1抗体(A-12)是小鼠单克隆IgG1 κ, 在4篇文献中引用，规格为200 µg/ml
  • 免疫human物种的NF-1的氨基酸1-300
  • 推荐用于 mouse, rat 和 human 来源的all NF-1 isoforms WB, IP, IF, IHC(P) 和 ELISA检测
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-74445 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 NF-1 抗体 (A-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NF-1 抗体 (A-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 NF-1 抗体包括 NF-1A

NF-1抗体（A-12）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的NF-1。NF-1，也称为CTF，是CCAAT盒结合蛋白家族的重要成员，在刺激DNA复制和激活转录方面发挥着重要作用。NF-1以同二聚体的形式与特定的DNA元件结合，利用氨基端DNA结合结构域，并通过独特的富含脯氨酸的羧基端转激活结构域激活转录。NF-1已被证明与2型腺病毒启动子相互作用，并参与单纯疱疹病毒胸腺嘧啶激酶基因的转录激活。NF-1识别的共识元件存在于许多真核基因的上游启动子区域，包括Ha-Ras、α-珠蛋白、HSP 70、GRP 78、组蛋白H1和髓磷脂碱性蛋白，以及非洲爪蟾卵黄蛋白基因的启动子。抗NF-1抗体（A-12）在各种应用中的多功能性使其成为研究人员在不同生物学背景下研究基因调控和蛋白质相互作用的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NF-1 抗体 (A-12) 参考文献:

  1. Ras/Raf/ERK信号传导与NF1。  |  Harrisingh, MC. and Lloyd, AC. 2004. Cell Cycle. 3: 1255-8. PMID: 15467460
  2. 1型神经纤维瘤病（NF-1）及相关肿瘤。  |  Rosenbaum, T. and Wimmer, K. 2014. Klin Padiatr. 226: 309-15. PMID: 25062113
  3. 肿瘤综合征和黑色素瘤中的NF1基因。  |  Kiuru, M. and Busam, KJ. 2017. Lab Invest. 97: 146-157. PMID: 28067895
  4. 1型神经纤维瘤病（NF1）中的人类干细胞建模。  |  Wegscheid, ML., et al. 2018. Exp Neurol. 299: 270-280. PMID: 28392281
  5. 易患嗜铬细胞瘤的遗传性疾病（VHL、NF-1、副神经节瘤综合征和新基因）。  |  Sarkadi, B. and Patócs, A. 2019. Exp Suppl. 111: 129-147. PMID: 31588531
  6. NF1的非肿瘤神经放射学表现及其临床意义。  |  Russo, C., et al. 2021. Cancers (Basel). 13: PMID: 33921292
  7. 癌症中的NF1改变：精准医疗中的治疗意义。  |  Giraud, JS., et al. 2023. Expert Opin Investig Drugs. 32: 941-957. PMID: 37747491
  8. 他汀类药物对改善1型神经纤维瘤病（NF-1）患儿认知障碍的疗效：荟萃分析。  |  Gan, L., et al. 2023. Front Pediatr. 11: 1274972. PMID: 37876519
  9. 曲美替尼对NF1患者神经心理特征的影响。  |  Lalancette, E., et al. 2024. J Neurooncol. 167: 447-454. PMID: 38443693
  10. NF1相关肿瘤的过去、现在和未来治疗策略。  |  Na, B., et al. 2024. Curr Oncol Rep. 26: 706-713. PMID: 38709422

订购信息

产品名称产品编号规格价格数量收藏夹

NF-1 抗体 (A-12)

sc-74445
200 µg/ml
RMB2377.00

NF-1 (A-12): m-IgG Fc BP-HRP 套装

sc-539520
200 µg Ab; 10 µg BP
RMB2662.00

NF-1 (A-12): m-IgG1 BP-HRP 套装

sc-541541
200 µg Ab; 20 µg BP
RMB2662.00

NF-1 抗体 (A-12) X

sc-74445 X
200 µg/0.1 ml
RMB2377.00

what isoform of NF1 detected by this antibody? why NF1 protein shown in WB is about 70K as it is know NF1 protein is about 300K in molecular weight.

Asked by: Anna
Thank you for your question. NF1 has a calculated molecular weight of approximately 47 kDa and is observable at higher molecular weights in western blots due to a number of post-translational modifications. The antibody is capable of detecting all NF-1 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2024-07-25

When should the TransCruz reagent sc-74445 X be used instead of the standard form sc-74445?

Asked by: cjMara
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-25

Is this against NFI (Nuclear Factor I) or NF-1 (neurofibromin-1)?  If the former, which family member NFIA, NFIB, NFIC or NFIX?

Asked by: rich
Thank you for your question. This antibody detects NF-1, Nuclear Factor I, and it will detect all the isoforms. If you go to the product block and click on the "gene info" tab, you can see which isoforms it detects (NFIA, NFIB, NFIC and NFIX).
Answered by: Tech Service
Date published: 2016-10-21
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Rated 4 out of 5 by from Robust band detected in WB using Jurkat wholeRobust band detected in WB using Jurkat whole cell lysate. -SCBT QC
Date published: 2015-07-08
Rated 4 out of 5 by from Bands seen in WB using HeLa and NIH/3T3 nuclearBands seen in WB using HeLa and NIH/3T3 nuclear extracts. -SCBT QC
Date published: 2015-05-26
Rated 5 out of 5 by from Beautiful immunoperoxidase nuclear stainingBeautiful immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-14
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-01-17
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NF-1 抗体 (A-12) is rated 4.3 out of 5 by 4.
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