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NF-1 Antibody (D-2) - Western Blotting - Image 356927 — NF-1 抗体 (D-2): sc-74444. 对 MCF7 (A), A-431 (B), L929 (C), Sol8 (D), RAT2 (E) 和 L8 (F) 全细胞裂解液中 NF-1 表达的 Western 印迹分析.

NF-1 抗体 (D-2): sc-74444. 对 MCF7 (A), A-431 (B), L929 (C), Sol8 (D), RAT2 (E) 和 L8 (F) 全细胞裂解液中 NF-1 表达的 Western 印迹分析.

NF-1 抗体 (D-2): sc-74444

Name: NF-1抗体 (D-2)
Brand: SCBIO
SKU: sc-74444
Price: 2377.0 CNY
Availability: InStock

3.6(10)

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说明书

NF-1 抗体 D-2 是小鼠单克隆 IgG₁ κ，NF-1抗体, 在5篇文献中引用，规格为200 µg/ml
免疫human物种的NF-1的氨基酸1-300
NF-1 抗体 (D-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 all NF-1 isoforms
抗NF-1抗体（D-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
可用于凝胶迁移和ChIP的TransCruz试剂(sc-74444 X, 200 µg/0.1 ml)
关于如何获取NF-1 (D-2): sc-74444的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP, m-IgG₁ BP-HRP 和 m-IgGκ BP-HRP 是 NF-1 抗体 (D-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NF-1 抗体 (D-2) 组合成套装提供。 (请参阅下面的订购信息)。

查看产品引用文献(5)

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NF-1 抗体 (D-2) 参考文献:

NF-1 因子在调节弹性蛋白基因转录中的作用。 | Degterev, A. and Foster, JA. 1999. Matrix Biol. 18: 295-307. PMID: 10429948
纯化的辅助因子和组蛋白 H1 介导 CTF/NF-I 的转录调控。 | Dusserre, Y. and Mermod, N. 1992. Mol Cell Biol. 12: 5228-37. PMID: 1406693
故障蛋白, 糖基化阻滞和钙离子诱导剂对 grp78 启动子的反式激活是通过含有 CCAAT 基调的近端区域介导的，该基调与 CTF/NF-I 相互作用。 | Wooden, SK., et al. 1991. Mol Cell Biol. 11: 5612-23. PMID: 1656235
分离出编码小脑丰富核因子 I 家族的互补 DNA，该家族可激活小鼠髓鞘碱性蛋白启动子的转录。 | Inoue, T., et al. 1990. J Biol Chem. 265: 19065-70. PMID: 1699939
CTF/NF-I 富含脯氨酸的转录激活因子与复制和 DNA 结合结构域不同。 | Mermod, N., et al. 1989. Cell. 58: 741-53. PMID: 2504497
一种激活真核生物转录和 DNA 复制的细胞 DNA 结合蛋白。 | Jones, KA., et al. 1987. Cell. 48: 79-89. PMID: 3024847
活跃于转录和 DNA 复制的人类 CCAAT-box 结合蛋白家族：多个 cDNA 的克隆和表达。 | Santoro, C., et al. 1988. Nature. 334: 218-24. PMID: 3398920
修订后的诊断标准（包括对致病性NF1基因变异的基因检测）为诊断幼儿1型神经纤维瘤病（NF1）提供了便利。 | Kehrer-Sawatzki, H. and Cooper, DN. 2022. Hum Genet. 141: 177-191. PMID: 34928431
两种转录激活因子--CCAAT-框结合转录因子和热休克转录因子--与人类 hsp70 基因启动子相互作用。 | Morgan, WD., et al. 1987. Mol Cell Biol. 7: 1129-38. PMID: 3561411
1型神经纤维瘤病（NF-1）的流行病学和结果：多中心三级经验。 | Almuqbil, M., et al. 2024. J Multidiscip Healthc. 17: 1303-1314. PMID: 38533410
使用MEK抑制剂治疗的NF1患者眼科并发症的发生率。 | Hummel, L., et al. 2024. Curr Oncol. 31: 2644-2649. PMID: 38785480
肝脏特异性卵黄素基因启动子中 CTF/NF-I, C/EBP 和 HNF3 结合位点的复杂组织。 | Cardinaux, JR., et al. 1994. J Biol Chem. 269: 32947-56. PMID: 7806524

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
NF-1 抗体 (D-2)	sc-74444	200 µg/ml	RMB2377.00
NF-1 (D-2): m-IgG Fc BP-HRP 套装	sc-528666	200 µg Ab; 10 µg BP	RMB2662.00
NF-1 (D-2): m-IgGκ BP-HRP 套装	sc-521113	200 µg Ab, 40 µg BP	RMB2662.00
NF-1 (D-2): m-IgG₁ BP-HRP 套装	sc-543059	200 µg Ab; 20 µg BP	RMB2662.00
NF-1 抗体 (D-2) X	sc-74444 X	200 µg/0.1 ml	RMB2377.00
NF-1 抗体 (D-2) AC	sc-74444 AC	500 µg/ml, 25% agarose	RMB3129.00
NF-1 抗体 (D-2) HRP	sc-74444 HRP	200 µg/ml	RMB2377.00
NF-1 抗体 (D-2) FITC	sc-74444 FITC	200 µg/ml	RMB2482.00
NF-1 抗体 (D-2) PE	sc-74444 PE	200 µg/ml	RMB2580.00
NF-1 抗体 (D-2) Alexa Fluor^® 488	sc-74444 AF488	200 µg/ml	RMB2685.00
NF-1 抗体 (D-2) Alexa Fluor^® 546	sc-74444 AF546	200 µg/ml	RMB2685.00
NF-1 抗体 (D-2) Alexa Fluor^® 594	sc-74444 AF594	200 µg/ml	RMB2685.00
NF-1 抗体 (D-2) Alexa Fluor^® 647	sc-74444 AF647	200 µg/ml	RMB2685.00
NF-1 抗体 (D-2) Alexa Fluor^® 680	sc-74444 AF680	200 µg/ml	RMB2685.00
NF-1 抗体 (D-2) Alexa Fluor^® 790	sc-74444 AF790	200 µg/ml	RMB2685.00

Are there any publications showing successful ChIP experiments using the TransCruz version of this antibody?

Asked by: melaniegirard

Thank you for your question. This is a relatively new monoclonal antibody and we are not aware of any publications using this antibody for ChIP at this time.

Answered by: Technical Support

Date published: 2018-11-20

This antibody is stated to recognize all isoforms of NF-1. Does this mean it recognize all isoforms of one of the four NF-1 genes (i.e. NF1-A, -B, -C and -X) or that it recognizes all four different NF-1 genes (NF1-A, -B, -C and -X)?

Asked by: Dr Ed

Thank you for your question. NF-1 Antibody (D-2): sc-74444 is a mouse monoclonal raised against amino acids 1-300 of NF1C of human origin. Based on sequence analysis, the antibody is expected to react with four different NF-1 genes (NFIA, NFIB, NFIC and NFIX). The protein sequences are highly conserved in this region.

Answered by: Technical Support

Date published: 2017-04-11

When should the TransCruz reagent sc-74444 X be used instead of the standard form sc-74444?

Asked by: Professor Griffin

Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.

Answered by: Technical Support

Date published: 2017-02-27

Rated 4 out of 5 by laurap230575 from buon anticorpoQuesto anticorpo dà un bel segnale, netto e specifico.

Date published: 2020-11-04

Rated 1 out of 5 by Melaniegirard from Unspecific bindingStrong unspecific band around 100 kb in THP-1 U937 and human monocytes. (reducing conditions)

Date published: 2018-12-11

Rated 2 out of 5 by Geroge from no good signal in IFI didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)

Date published: 2018-03-07

Rated 5 out of 5 by nz0103 from Worked well for WBI used nuclear extract from my mouse samples and did 1:1000 dilution for WB. It worked well!

Date published: 2018-01-11

Rated 1 out of 5 by Alex Nesta from Unfortunately did not work on mouse brain tissueWe tried Immunofluorescence (1:200) on cryosections from 4% PFA fixed embryonic mouse brain tissue. 3-5 min retrieval with citric buffer (PH6.0). Unfortunately we saw no signal.

Date published: 2017-11-09

Rated 5 out of 5 by karpov from Great resultsThe NF-1 Antibody works very well for Western blot applications, showing strong and clear banding with minimal background noise present

Date published: 2017-08-29

Rated 5 out of 5 by davay from GreatThis antibody is pretty good for IF staining. The background is very low.

Date published: 2017-08-02

Rated 4 out of 5 by JL from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC

Date published: 2015-06-26

NF-1 抗体 (D-2) Reviews - page 2

NF-1 抗体 (D-2) is rated 3.6 out of 5 by 10.

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版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。

种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	NFIB	Entrez Gene ID: 4781	9p23	XM_005251467, XM_005251468, XM_005251469, XM_005251470, XM_005251471, XM_006716773, XM_006716774, XM_006716775, XM_017014739, XM_017014740, XM_017014741, XM_017014742, NM_001190737, NM_001190738, NM_001282787, NM_005596	UniProt ID: O00712	600728
Human	NFIX	Entrez Gene ID: 4784	19p13.2	XM_005259917, XM_005259918, XM_005259919, XM_005259920, XM_006722760, XM_011528040, XM_017026837, NM_001271043, NM_001271044, NM_002501	UniProt ID: Q14938	164005
Human	NFIC	Entrez Gene ID: 4782	19p13.3	XM_005259564, XM_006722759, XM_017026834, XM_017026835, XM_017026836, NM_001245002, NM_001245004, NM_001245005, NM_005597, NM_205843	UniProt ID: P08651	600729
Human	NFIA	Entrez Gene ID: 4774	1p31.3	XM_011541512, XM_011541514, XM_011541515, XM_017001362, XM_017001363, NM_001134673, NM_001145511, NM_001145512, NM_005595	UniProt ID: Q12857	600727
Mouse	Nfia	Entrez Gene ID: 18027	4 C6	XM_006502841, XM_006502842, XM_006502843, XM_006502844, XM_006502845, NM_001122952, NM_001122953, NM_010905	UniProt ID: Q02780	N/A
Mouse	Nfib	Entrez Gene ID: 18028	4 C3	XM_006537653, XM_006537654, XM_017320021, XM_017320022, NM_001113209, NM_001113210, NM_001286127, NM_001286131, NM_008687	UniProt ID: P97863	N/A
Mouse	Nfix	Entrez Gene ID: 18032	8 C3	XM_006530755, XM_006530756, XM_006530757, XM_006530758, XM_006530759, XM_006530760, XM_006530761, XM_006530762, XM_006530763, XM_011248326, NM_001081981, NM_001081982, NM_001297601, NM_010906	UniProt ID: P70257	N/A
Mouse	Nfic	Entrez Gene ID: 18029	10 C1	XM_006513335, NM_008688, NM_026756	UniProt ID: P70255	N/A

NF-1 抗体 (D-2): sc-74444

NF-1 抗体 (D-2) 参考文献:

NF-1 抗体 (D-2) 规格

替代产品名称: NFIB

产品克隆名称: D-2

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 1-300

抗原表位靶标: NF-1

抗原表位种属: human

产品反应性/检测: all NF-1 isoforms

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 5篇各种科学出版物的引文

NF-1 抗体 (D-2)

NF-1 (D-2): m-IgG Fc BP-HRP 套装

NF-1 (D-2): m-IgGκ BP-HRP 套装

NF-1 (D-2): m-IgG₁ BP-HRP 套装

NF-1 抗体 (D-2) X

NF-1 抗体 (D-2) AC

NF-1 抗体 (D-2) HRP

NF-1 抗体 (D-2) FITC

NF-1 抗体 (D-2) PE

NF-1 抗体 (D-2) Alexa Fluor^® 488

NF-1 抗体 (D-2) Alexa Fluor^® 546

NF-1 抗体 (D-2) Alexa Fluor^® 594

NF-1 抗体 (D-2) Alexa Fluor^® 647

NF-1 抗体 (D-2) Alexa Fluor^® 680

NF-1 抗体 (D-2) Alexa Fluor^® 790

Are there any publications showing successful ChIP experiments using the TransCruz version of this antibody?

This antibody is stated to recognize all isoforms of NF-1. Does this mean it recognize all isoforms of one of the four NF-1 genes (i.e. NF1-A, -B, -C and -X) or that it recognizes all four different NF-1 genes (NF1-A, -B, -C and -X)?

When should the TransCruz reagent sc-74444 X be used instead of the standard form sc-74444?

NF-1 抗体 (D-2): sc-74444

NF-1 抗体 (D-2) 参考文献:

NF-1 抗体 (D-2) 规格

替代产品名称: NFIB

产品克隆名称: D-2

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: 1-300

抗原表位靶标: NF-1

抗原表位种属: human

产品反应性/检测: all NF-1 isoforms

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 5篇各种科学出版物的引文

NF-1 抗体 (D-2)

NF-1 (D-2): m-IgG Fc BP-HRP 套装

NF-1 (D-2): m-IgGκ BP-HRP 套装

NF-1 (D-2): m-IgG1 BP-HRP 套装

NF-1 抗体 (D-2) X

NF-1 抗体 (D-2) AC

NF-1 抗体 (D-2) HRP

NF-1 抗体 (D-2) FITC

NF-1 抗体 (D-2) PE

NF-1 抗体 (D-2) Alexa Fluor® 488

NF-1 抗体 (D-2) Alexa Fluor® 546

NF-1 抗体 (D-2) Alexa Fluor® 594

NF-1 抗体 (D-2) Alexa Fluor® 647

NF-1 抗体 (D-2) Alexa Fluor® 680

NF-1 抗体 (D-2) Alexa Fluor® 790

Are there any publications showing successful ChIP experiments using the TransCruz version of this antibody?

This antibody is stated to recognize all isoforms of NF-1. Does this mean it recognize all isoforms of one of the four NF-1 genes (i.e. NF1-A, -B, -C and -X) or that it recognizes all four different NF-1 genes (NF1-A, -B, -C and -X)?

When should the TransCruz reagent sc-74444 X be used instead of the standard form sc-74444?

抗体同型: IgG₁ kappa light chain

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

NF-1 (D-2): m-IgG₁ BP-HRP 套装

NF-1 抗体 (D-2) Alexa Fluor^® 488

NF-1 抗体 (D-2) Alexa Fluor^® 546

NF-1 抗体 (D-2) Alexa Fluor^® 594

NF-1 抗体 (D-2) Alexa Fluor^® 647

NF-1 抗体 (D-2) Alexa Fluor^® 680

NF-1 抗体 (D-2) Alexa Fluor^® 790