Neurofibromin 抗体 (McNFn27a): sc-20017

Name: Neurofibromin抗体 (McNFn27a)
Brand: SCBIO
SKU: sc-20017
Price: 2377.0 CNY
Availability: InStock

3.3(3)

寫評論提問

说明书

Neurofibromin抗体(McNFn27a)是小鼠单克隆IgG₁ κ, 在4篇文献中引用，规格为200 µg/ml
与human的Neurofibromin的N-terminal肽
推荐用于 mouse, rat 和 human 来源的Neurofibromin WB, IP, IF 和 IHC(P)检测
看 Neurofibromin (H-12): sc-376886，可查看有标记的 Neurofibromin 抗体，包括AC, HRP, FITC, PE, Alexa Fluor^® 488, 594, 647, 680 和 790
m-IgG Fc BP-HRP, m-IgG₁ BP-HRP 和 m-IgGκ BP-HRP 是 Neurofibromin 抗体 (McNFn27a) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Neurofibromin 抗体 (McNFn27a) 组合成套装提供。 (请参阅下面的订购信息)。

查看产品引用文献(4)

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I am using Neurofibromin (McNFn27a): sc-20017 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin

We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by ekahen from This antibody genuinely *does* work (even on WB)Hi. I am first author of one of the cited publications for this antibody (at least my pub still listed as 08/2025; Kahen, E. et al.) and I noticed someone else said they could not get signal on WB in their hands. I want to reassure that you really can get great WB's with this antibody for any human cell line, but it is a labor of love. Neurofibromin is a fairly large protein as you likely know, (typically considered 240 kd) often running somewhere around 210-240 kda range on a gel per MW markers. As I wrote in my methods, I had to use a tris-acetate gradient gel of 3-8% to capture NF1 and its putative isoform explicitly (Very painful gel to deal with; many gels were broken during troubleshooting). Getting the loading control was tricky because tubulin would run around the same range as a non-specific band. Alpha-actinin ran *very close* to a non-specific band so was not "publication worthy" but was good for testing, and was used, and others might benefit from this using alpha-actinin for loading control for NF1 if new antibodies more specific. I had to find perfect timing to be able to capture proper *actin* right at the bottom of the gel, which has far more specific WB antibodies for pub-worthy images. Note: Wet transfer 100% necessary to get all the NF1. Dry transfer would either over-transfer the actin or (most often) under-transfer the NF1. Very difficult to get sufficient NF1 on a membrane via dry-transfer without losing other bands for controls (and I was also really trying to get RAS on same gel). Honestly I think I legit tried dry transfer for a hilarious amount of time once and when I stained the gel out of curiosity, there was *still* so much protein in the NF1 region. I really tried many times with dry transfer- wet transfer only way to get anything useful. Then, I actually blocked membranes *overnight* in Odyssey blocking buffer. This Santa-Cruz NF1 antibody was then used at highest concentration recommended by manufacturer, again in Odyssey blocking buffer *overnight* (4degC on shaker). Very thorough washing in TBST (and I think I actually preferred lower tween 20 % but 10 min washes). I really wanted clean crisp neurofibromin showing two bands, as I did. And ultimately, I was able to get the result I wanted and reproduce it (though actin could really have looked a little prettier, but quantified accurately overall.) I suppose we should really be this detailed in our methods, explaining these sorts of things, but we tend not to be. Nonetheless, what I've described might help someone working with this antibody. Feel free to reach out via email and I will try to remember my best other than what I've written here.

Date published: 2025-08-02

Rated 1 out of 5 by Yan Cui from Not working for WB in our handsessentially no specific bands, nor non-specific bands.

Date published: 2019-05-29

Rated 4 out of 5 by HC from WB: Worked well in mouse spleen samplesWB: Worked well in mouse spleen samples. -SCBT Publication Review

Date published: 2015-06-18

Rated 5 out of 5 by SH from Published data for IF in human Schwann cellsPublished data for IF in human Schwann cells and CCF cells. -SCBT Publication Review

Date published: 2013-05-05

Neurofibromin 抗体 (McNFn27a) is rated 3.8 out of 5 by 4.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	NF1	Entrez Gene ID: 4763	17q11.2	XM_005257983, XM_005257984, XM_006721922, XM_006721924, XM_006721925, XM_006721926, XM_011524852, XM_011524857, XM_017024689, XM_017024690, NM_000267, NM_001042492, NM_001128147	UniProt ID: P21359	607785
Mouse	Nf1	Entrez Gene ID: 18015	11 B5	XM_006532436, XM_006532437, XM_006532438, XM_006532439, XM_006532440, XM_006532441, XM_006532442, NM_010897	UniProt ID: Q04690	N/A

产品名称	产品编号	规格	价格	数量	收藏夹
Neurofibromin 抗体 (McNFn27a)	sc-20017	200 µg/ml	RMB2377.00
Neurofibromin (McNFn27a): m-IgG Fc BP-HRP 套装	sc-536640	200 µg Ab; 10 µg BP	RMB2662.00
Neurofibromin (McNFn27a): m-IgGκ BP-HRP 套装	sc-533797	200 µg Ab; 40 µg BP	RMB2662.00
Neurofibromin (McNFn27a): m-IgG₁ BP-HRP 套装	sc-544725	200 µg Ab; 20 µg BP	RMB2662.00

Neurofibromin 抗体 (McNFn27a): sc-20017

優選的 Neurofibromin 抗體是 Neurofibromin 抗體 (H-12): sc-376886.

Neurofibromin 抗体 (McNFn27a)

Neurofibromin (McNFn27a): m-IgG Fc BP-HRP 套装

Neurofibromin (McNFn27a): m-IgGκ BP-HRP 套装

Neurofibromin (McNFn27a): m-IgG1 BP-HRP 套装

I am using Neurofibromin (McNFn27a): sc-20017 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Neurofibromin (McNFn27a): m-IgG₁ BP-HRP 套装