Date published: 2025-9-8
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Neurofibromin Antibody (H-12) - Western Blotting - Image 160900
神经纤维蛋白抗体 (H-12): sc-376886. Western 印迹分析 LNCaP 全细胞裂解液中 Neurofibromin 的表达.
神经纤维蛋白抗体 (H-12): sc-376886. Western 印迹分析 LNCaP 全细胞裂解液中 Neurofibromin 的表达.

Neurofibromin 抗体 (H-12): sc-376886

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说明书
  • Neurofibromin 抗体 H-12 是小鼠单克隆 IgG1 κ，Neurofibromin抗体, 在16篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列241-540位于human物种的Neurofibromin的N-terminus附近
  • Neurofibromin 抗体 (H-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Neurofibromin; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗Neurofibromin抗体（H-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Neurofibromin (H-12): sc-376886的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Neurofibromin 抗体 (H-12)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

神经纤维瘤蛋白抗体（H-12）是一种IgG1 κ小鼠单克隆神经纤维瘤蛋白抗体（也称为NF1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人来源的神经纤维瘤蛋白。神经纤维瘤蛋白抗体（H-12）既可作为非偶联抗神经纤维瘤蛋白抗体形式，也可作为多种偶联形式的抗神经纤维瘤蛋白抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。1型神经纤维瘤病（NF1），或von Reckinghausen神经纤维瘤病，是人类最常见的常染色体显性疾病之一。早期的连锁分析将NF1基因定位到17号染色体。预测的NF1转录本编码2818个氨基酸的蛋白质神经纤维瘤蛋白，也称为NF1-GAP相关蛋白（NF1GRP）。通过序列分析，已经证明神经纤维瘤蛋白的一小部分区域与Ras GAP基因家族成员具有相似性。在功能上，通过涉及RAS-GAP水解和在酵母中的功能互补的生化分析表明，神经纤维瘤蛋白进一步类似于GAP蛋白。神经纤维瘤蛋白在包括大脑、肺、肝、肾、脾、肌肉和结肠在内的多种细胞系和组织中以相对恒定的水平表达。尽管关于神经纤维瘤蛋白的功能知之甚少，但与具有GTPase活性的蛋白质的催化结构域的同源性表明，神经纤维瘤蛋白也可能在体内与Ras蛋白质相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Neurofibromin 抗体 (H-12) 参考文献:

  1. 关于神经纤维瘤蛋白的溶酶体降解及其在培养黑色素细胞中的磷酸化。  |  Kaufmann, D., et al. 1999. Biol Chem. 380: 1071-8. PMID: 10543444
  2. 镶嵌型神经纤维瘤病 1 型孤立疾病特征的分子剖析。  |  Maertens, O., et al. 2007. Am J Hum Genet. 81: 243-51. PMID: 17668375
  3. 神经纤维瘤病 1 型前核糖核酸神经元特异性替代剪接的调控。  |  Zhu, H., et al. 2008. Mol Cell Biol. 28: 1240-51. PMID: 18086893
  4. 通过改变 RAS, RAF, NF1 和/或 RASSF1A 在结直肠癌中传递 RAS 信号。  |  Ahlquist, T., et al. 2008. Neoplasia. 10: 680-6, 2 p following 686. PMID: 18592002
  5. 鉴定 1 型神经纤维瘤病基因产品。  |  Gutmann, DH., et al. 1991. Proc Natl Acad Sci U S A. 88: 9658-62. PMID: 1946382
  6. 1 型神经纤维瘤病基因编码一种与 GAP 相关的蛋白质。  |  Xu, GF., et al. 1990. Cell. 62: 599-608. PMID: 2116237
  7. 神经纤维瘤病 1 型基因产物的催化结构域可刺激 ras GTPase 并补充 S. cerevisiae 的 ira 突变体。  |  Xu, GF., et al. 1990. Cell. 63: 835-41. PMID: 2121369
  8. 神经纤维瘤病 1 型基因产物的 GAP 相关结构域与 ras p21 相互作用。  |  Martin, GA., et al. 1990. Cell. 63: 843-9. PMID: 2121370
  9. 神经纤维瘤蛋白1是神经元中的miRNA靶标。  |  Paschou, M. and Doxakis, E. 2012. PLoS One. 7: e46773. PMID: 23056445
  10. I 型神经纤维瘤病的多点关联分析：一项国际合作。  |  Goldgar, DE., et al. 1989. Am J Hum Genet. 44: 6-12. PMID: 2491784
  11. 神经纤维瘤蛋白调节大鼠毛果芸香碱诱导的癫痫发作。  |  Ren, M., et al. 2016. Mol Neurobiol. 53: 6069-6077. PMID: 26537900
  12. 神经纤维瘤蛋白招募因子Spred1与GAP相关结构域结合，不影响Ras失活。  |  Dunzendorfer-Matt, T., et al. 2016. Proc Natl Acad Sci U S A. 113: 7497-502. PMID: 27313208

订购信息

产品名称产品编号规格价格数量收藏夹

Neurofibromin 抗体 (H-12)

sc-376886
200 µg/ml
RMB2377.00

Neurofibromin 抗体 (H-12) AC

sc-376886 AC
500 µg/ml, 25% agarose
RMB3129.00

Neurofibromin 抗体 (H-12) HRP

sc-376886 HRP
200 µg/ml
RMB2377.00

Neurofibromin 抗体 (H-12) FITC

sc-376886 FITC
200 µg/ml
RMB2482.00

Neurofibromin 抗体 (H-12) PE

sc-376886 PE
200 µg/ml
RMB2580.00

Neurofibromin 抗体 (H-12) Alexa Fluor® 488

sc-376886 AF488
200 µg/ml
RMB2685.00

Neurofibromin 抗体 (H-12) Alexa Fluor® 546

sc-376886 AF546
200 µg/ml
RMB2685.00

Neurofibromin 抗体 (H-12) Alexa Fluor® 594

sc-376886 AF594
200 µg/ml
RMB2685.00

Neurofibromin 抗体 (H-12) Alexa Fluor® 647

sc-376886 AF647
200 µg/ml
RMB2685.00

Neurofibromin 抗体 (H-12) Alexa Fluor® 680

sc-376886 AF680
200 µg/ml
RMB2685.00

Neurofibromin 抗体 (H-12) Alexa Fluor® 790

sc-376886 AF790
200 µg/ml
RMB2685.00

Neurofibromin Antibody (H-12): sc-376886 works but signal is very weak in both WB and IHC/IF. Do you have higher concentration version of this product available? If so, please give me a quote.

Asked by: alisonyw
Thank you for your question. We can provide this antibody at a higher dilution as a special order product. Please contact customer care and provide your shipping and billing addresses so we can send you a quote.
Answered by: Tech Support Europe
Date published: 2020-08-10

how come the mice anti-NF1 can be used for detection of protein in mice tissue as it is mentioned above in western blot samples for example? thanks

Asked by: Azsa
Thank you for your question. This antibody is recommended for detection of Neurofibromin of mouse, rat and human origin. There is representative data in mouse NIH/3T3 whole cell lysate by Western blot. If this does not answer your question, please contact us for further Technical Support.
Answered by: Technical Support
Date published: 2019-04-13

I would like to use Neurofibromin Antibody (H-12): sc-376886 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-376886 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 2 out of 5 by from faint bandwe tested this antibody and we obtained a faint band
Date published: 2021-03-24
Rated 4 out of 5 by from good but not excellent for WBSpecific signal at ~320 kDa, validated by knockdown and overexpression experiment. The sensitivity is not high, much lower than sc-67 (discontinued); there might be non-specific bands at ~85 kDa and ~120 kDa positions,
Date published: 2019-05-29
Rated 5 out of 5 by from Antibody detected neurofibrominNeurofibromin is a very large protein (over 300 kDa) and I tried several antibodies from different vendors in order to obtain a Western image of NF1 in 6 cell types. The Santa Cruz antibody worked very well and detected an appropriately sized protein on PVDF using a TAE 3%-8% gel and Licor IR secondary antibodies. The band is not very prominent but it's there in 5 of 6 cell lines (the 6th was actually expected to NOT have it).
Date published: 2019-04-03
Rated 3 out of 5 by from Some non-specific binding in IHCWe used this antibody for IHC in brain tissue. Since this is a mouse monoclonal antibody, we had a lot of trouble with blood vessel staining even after adequate perfusion. The stain definitely worked, however we need to work hard to work around the blood vessels.
Date published: 2017-12-13
Rated 5 out of 5 by from acceptableAlthough little is known about the function of neurofibromin in the final experimental results, we show that the homology of the catalytic domain of the protein with GTPase activity suggests that neurofibromin can also interact with Ras proteins in vivo.
Date published: 2017-02-02
Rated 5 out of 5 by from Great monoclonal choice for NeurofibrominThis (H-12) clone is an excellent replacement for the (H-300) polyclonal antibody. It has the same epitope, and excellent data in WB and IHC(P).
Date published: 2017-01-12
Rated 5 out of 5 by from Superb immunoperoxidase membrane stainingSuperb immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Nice Western Blot data of Neurofibromin expressionNice Western Blot data of Neurofibromin expression in LNCaP whole cell lysate. -SCBT QC
Date published: 2014-11-16
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Neurofibromin 抗体 (H-12) is rated 4.3 out of 5 by 8.
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