Date published: 2026-11-7

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ErbB2/HER2 Antibody (A-2) - Immunohistochemistry - Image 352337
Neu 抗体 (A-2): sc-393712. 福尔马林固定, 石蜡包埋的人子宫颈组织的免疫过氧化物酶染色显示鳞状上皮细胞的胞质, 膜和核染色.
Neu 抗体 (A-2): sc-393712. 福尔马林固定, 石蜡包埋的人子宫颈组织的免疫过氧化物酶染色显示鳞状上皮细胞的胞质, 膜和核染色.

ErbB2/HER2 抗体 (A-2): sc-393712

4.2(6)
寫評論提問
说明书
  • ErbB2/HER2抗体(A-2)是小鼠单克隆IgG1 κ, 在16篇文献中引用，规格为200 µg/ml
  • 特异性表位图谱分析在human的Neu的C-terminal细胞质域内的1180-1197氨基酸之间进行
  • 抗-ErbB2/HER2 抗体 (A-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Neu; 也和以下物种反应，包括： equine
  • 抗ErbB2/HER2抗体（A-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是ErbB2/HER2 Antibody (A-2) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与ErbB2/HER2 Antibody (A-2) 打包提供（请参阅下面的订购信息）。
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描述
基因信息
说明书与实验方案
研究信息

関連項目

Neu/ErbB2/HER2抗体（A-2）是一种IgG1 κ小鼠单克隆Neu抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Neu。Neu/ErbB2/HER2抗体（A-2）既可作为非偶联抗Neu抗体形式，也可作为多种偶联形式的抗Neu抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。EGF受体家族包括几种相关的受体酪氨酸激酶，这些激酶在各种癌中经常过表达。该受体家族的成员包括EGFR（HER1）、Neu（ErbB-2、HER2）、ErbB-3（HER3）和ErbB-4（HER4），它们在配体结合时形成同二聚体或异二聚体。Neu是一种糖蛋白，在与其他EGF受体家族成员异源二聚化后发生反式激活。Neu与ErbB-3的异源二聚化招募了heregulin，从而诱导磷酸肌醇（PI）3-激酶的激活。Neu的激活增强了肿瘤细胞的运动性、蛋白酶分泌和侵袭，还调节了细胞周期检查点功能、DNA修复和凋亡反应。Neu的扩增和/或过表达发生在20-30%的乳腺癌中。Neu表达增加的测量可以预测疾病预后。Neu也可能被证明是治疗药物的有希望的目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

含汞：所有HRP偶联物（包括组合装）均含有硫柳汞，这是一种汞化合物。请勿丢弃在垃圾中。应按照当地、州和联邦法规，将其作为危险化学品废弃物进行处置。

下载硫柳汞安全数据表（SDS）

ErbB2/HER2 抗体 (A-2) 参考文献:

  1. 鉴定和验证新型ERBB2（HER2、NEU）靶标，包括参与血管生成的基因。  |  Beckers, J., et al. 2005. Int J Cancer. 114: 590-7. PMID: 15609325
  2. 乳腺癌-1中扩增的核受体共激活因子是小鼠中Neu（ErbB2/HER2）激活、信号传导和乳腺肿瘤发生所必需的。  |  Fereshteh, MP., et al. 2008. Cancer Res. 68: 3697-706. PMID: 18483252
  3. LMO4是ErbB2/HER2/Neu诱导的乳腺癌细胞周期进展的重要介质。  |  Montañez-Wiscovich, ME., et al. 2009. Oncogene. 28: 3608-18. PMID: 19648968
  4. MET和ERBB2在ERBB2+乳腺癌中共同表达，并有助于先天抵抗。  |  Paulson, AK., et al. 2013. Mol Cancer Res. 11: 1112-21. PMID: 23825050
  5. 人类视网膜母细胞瘤中的HER2/ERBB2免疫反应。  |  Seigel, GM., et al. 2016. Tumour Biol. 37: 6135-42. PMID: 26614428
  6. 体内证据支持Stard13（Dlc2）在ErbB2（Neu）致癌基因诱导的小鼠乳腺肿瘤中具有抑制转移的作用。  |  Basak, P., et al. 2018. Genes Chromosomes Cancer. 57: 182-191. PMID: 29218825
  7. 电泳细胞病理学以单细胞分辨率解析了ERBB2形式。  |  Kang, CC., et al. 2018. NPJ Precis Oncol. 2: 10. PMID: 29872719
  8. ERBB2-YAP串扰介导小鼠心脏再生  |  Fernández-Ruiz, I. 2021. Nat Rev Cardiol. 18: 4. PMID: 33110243
  9. 自噬抑制可干扰ERBB2的运输，并消除ERBB2驱动的乳腺癌的肿瘤形成。  |  Hao, M., et al. 2021. Autophagy. 17: 1059-1060. PMID: 33757386
  10. 双特异性抗体用于将干扰素-β传递到ErbB2+肿瘤。  |  Rybchenko, VS., et al. 2021. Biomolecules. 11: PMID: 34944558

订购信息

产品名称产品编号规格价格数量收藏夹

ErbB2/HER2 抗体 (A-2)

sc-393712
200 µg/ml
CNY2422.00

ErbB2/HER2 (A-2): m-IgG Fc BP-HRP 套装

sc-530553
200 µg Ab; 10 µg BP
CNY2715.00

ErbB2/HER2 (A-2): m-IgGκ BP-HRP 套装

sc-524059
200 µg Ab, 40 µg BP
CNY2715.00

ErbB2/HER2 (A-2): m-IgG1 BP-HRP 套装

sc-544124
200 µg Ab; 20 µg BP
CNY2715.00

ErbB2/HER2 抗体 (A-2) AC

sc-393712 AC
500 µg/ml, 25% agarose
CNY3189.00

ErbB2/HER2 抗体 (A-2) HRP

sc-393712 HRP
200 µg/ml
CNY2422.00

ErbB2/HER2 抗体 (A-2) FITC

sc-393712 FITC
200 µg/ml
CNY2527.00

ErbB2/HER2 抗体 (A-2) PE

sc-393712 PE
200 µg/ml
CNY2625.00

ErbB2/HER2 抗体 (A-2) Alexa Fluor® 488

sc-393712 AF488
200 µg/ml
CNY2738.00

ErbB2/HER2 抗体 (A-2) Alexa Fluor® 546

sc-393712 AF546
200 µg/ml
CNY2738.00

ErbB2/HER2 抗体 (A-2) Alexa Fluor® 594

sc-393712 AF594
200 µg/ml
CNY2738.00

ErbB2/HER2 抗体 (A-2) Alexa Fluor® 647

sc-393712 AF647
200 µg/ml
CNY2738.00

ErbB2/HER2 抗体 (A-2) Alexa Fluor® 680

sc-393712 AF680
200 µg/ml
CNY2738.00

ErbB2/HER2 抗体 (A-2) Alexa Fluor® 790

sc-393712 AF790
200 µg/ml
CNY2738.00

ErbB2/HER2 (A-2) 中和勝肽

sc-393712 P
100 µg/0.5 ml
CNY519.00

What application is the blocking peptide sc-393712 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by Alessia UNITO from did not work for immunofluorescence (pig)Immunohistochemistry: the antibody was tested on mammary parenchyma and small intestine from 6 months old gilts. Antigen retrieval was performed in citrate buffer, 30'. Primary antibody: 1:50 dilution, 1h, RT. Secondary antibody: Alexa Fluor 488 (1:500), 1h, RT. Results: negative; nonspecific signal was observed both in epithelial and connective fractions. Immunocytochemistry: the antibody was tested on porcine mammary epithelial cells isolated from 6 months old gilts. Cells were fixed with acetone-methanol (1:1) and stained as above (see immunohistochemistry). Primary antibody was diluted 1:100. Result: negative.
Date published: 2021-02-01
Rated 4 out of 5 by Christiana from Good signalWe tried it at the specs recommended by the company, gives a strong signal and seems at the right hight
Date published: 2018-03-26
Rated 5 out of 5 by jenniferc from Outstanding antibody!Neu Antibody (A-2) produced outstanding results. Very strong signal was observed by WB and IHCP
Date published: 2017-01-21
Rated 5 out of 5 by Ahmed AlShammari from PerfectI used this antibody in immunocytochemistry and it was very good
Date published: 2016-12-13
Rated 5 out of 5 by Susan from We used this in WB and IP in human cell linesWe used this in WB and IP in human cell lines and works beautifully.
Date published: 2015-10-20
Rated 5 out of 5 by LR from Very good Western blot data of Neu expressionVery good Western blot data of Neu expression in SK-BR-3 and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-08-26
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ErbB2/HER2抗体 (A-2) is rated 4.2 out of 5 by 6.
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