Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
ErbB2/HER2 Antibody (3B5) - Western Blotting - Image 362025
Neu 抗体 (3B5): sc-33684. A-431 (A), OVCAR-3 (B) 和 NIH/3T3 (C) 全细胞裂解液中 Neu 表达的 Western 印迹分析.
Neu 抗体 (3B5): sc-33684. A-431 (A), OVCAR-3 (B) 和 NIH/3T3 (C) 全细胞裂解液中 Neu 表达的 Western 印迹分析.

ErbB2/HER2 抗体 (3B5): sc-33684

4.4(35)
寫評論提問

说明书
  • ErbB2/HER2 抗体 3B5 是小鼠单克隆 IgG1 κ，ErbB2/HER2抗体, 在73篇文献中引用，规格为200 µg/ml
  • 与human的Neu的氨基酸1242-1255对应的合成肽杂交
  • ErbB2/HER2 抗体 (3B5) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Neu
  • 抗ErbB2/HER2抗体（3B5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ErbB2/HER2 (3B5): sc-33684的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 ErbB2/HER2 抗体 (3B5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ErbB2/HER2 抗体 (3B5) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

Neu抗体（3B5）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测小鼠、大鼠和人类来源的Neu蛋白。抗 -Neu抗体（3B5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Neu，也称为ErbB-2或HER2，是表皮生长因子受体（EGFR）家族的一员，该家族由受体酪氨酸激酶组成，在细胞生长和分化相关的信号传导通路中发挥关键作用。Neu主要位于细胞膜上，与配体结合后可与其他EGFR家族成员（如ErbB-3）形成同二聚体或异二聚体。这种二聚化作用可激活下游信号级联，包括磷脂酰肌醇3激酶通路，从而调节细胞的增殖、存活和运动。Neu的异常表达与多种癌症有关，尤其是乳腺癌，其中20-30%的病例中会发生扩增或过度表达，这使得Neu成为疾病预后的重要生物标志物和治疗干预的潜在靶点。Neu单克隆抗体（3B5）使研究人员能够深入了解Neu在癌症生物学中的作用和治疗潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

ErbB2/HER2 抗体 (3B5)

sc-33684
200 µg/ml
RMB2377.00

ErbB2/HER2 (3B5): m-IgG Fc BP-HRP 套装

sc-528486
200 µg Ab; 10 µg BP
RMB2662.00

ErbB2/HER2 (3B5): m-IgGκ BP-HRP 套装

sc-520877
200 µg Ab, 40 µg BP
RMB2662.00

ErbB2/HER2 (3B5): m-IgG1 BP-HRP 套装

sc-542962
200 µg Ab; 20 µg BP
RMB2662.00

ErbB2/HER2 抗体 (3B5) AC

sc-33684 AC
500 µg/ml, 25% agarose
RMB3129.00

ErbB2/HER2 抗体 (3B5) HRP

sc-33684 HRP
200 µg/ml
RMB2377.00

ErbB2/HER2 抗体 (3B5) FITC

sc-33684 FITC
200 µg/ml
RMB2482.00

ErbB2/HER2 抗体 (3B5) PE

sc-33684 PE
200 µg/ml
RMB2580.00

ErbB2/HER2 抗体 (3B5) Alexa Fluor® 488

sc-33684 AF488
200 µg/ml
RMB2685.00

ErbB2/HER2 抗体 (3B5) Alexa Fluor® 546

sc-33684 AF546
200 µg/ml
RMB2685.00

ErbB2/HER2 抗体 (3B5) Alexa Fluor® 594

sc-33684 AF594
200 µg/ml
RMB2685.00

ErbB2/HER2 抗体 (3B5) Alexa Fluor® 647

sc-33684 AF647
200 µg/ml
RMB2685.00

ErbB2/HER2 抗体 (3B5) Alexa Fluor® 680

sc-33684 AF680
200 µg/ml
RMB2685.00

ErbB2/HER2 抗体 (3B5) Alexa Fluor® 790

sc-33684 AF790
200 µg/ml
RMB2685.00

In a previous question you recommend performing antigen retrieval with sodium citrate buffer (pH 6) for Neu (3B5) and heat, but which antibody dilution do you recomend for FFPE tissue sections that don´t overexpress the receptor?

Asked by: MiguelC
Thank you for your question. We recommend a starting dilution of 1:50 for IHC applications, but conditions do need to be optimized for each antibody on each sample. We do recommend antigen retrieval for all formalin fixed samples.
Answered by: Tech Service
Date published: 2019-10-22

Does Neu(3B5) Ab recognize the phospho-Neu or the non phosphorilated one?

Asked by: vall
Thank you for your question. This antibody is not phospho-specific. It is a total Neu antibody, able to detect both non-phosphorylated and phosphorylated forms of Neu. Please see the Western blot data on our website demonstrating this reactivity in Neuregulin-1 treated, and lambda protein phosphatase treated MCF-7 whole cell lysates.
Answered by: Technical Support
Date published: 2024-05-29

Detektiert der Ak sc-33684 das Molakül ErbB2 im extrazellulären Teil, also bei intakten Zellen?

Asked by: FDMGV007
Vielen Dank für Ihre Anfrage und Ihr Interesse an diesem Produkt. Der Neu Antikörper (3B5): sc-33684 wurde gegen ein synthetisches Peptid gezogen, welches der Region AS 1242-1255 des humanen ERBB2 (Neu) Proteins entspricht. Diese befindet sich in der zytoplasmatischen Domäne des ERBB2 Proteins (AS 676 – 1255 laut uniprot). Der Antikörper sollte daher den extrazellulären Teil des Protein vermutlich nicht erkennen. Für weitere Informationen wenden Sie sich bitte an unseren Technischen Service in unserem Europäischen Supportbüro in Heidelberg. Sie erreichen uns telefonisch unter 06221 4503 0, per email an: [email protected] oder per Live-Chat auf unserer Webseite www.scbt.com Wir freuen uns Ihnen weiterzuhelfen.
Answered by: Technical Support Europe
Date published: 2017-07-06

Can Neu (3B5): sc-33684 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, Neu (3B5): sc-33684 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 3
Date published: 2023-07-26
  • y_2025, m_9, d_6, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_33684, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 109ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Buon anticorpoAnticorpo che funziona bene per western blot senza bande aspecifiche
Date published: 2024-10-17
Rated 5 out of 5 by from ASSISTANT PROFESSORWe request free sample for this antibodies months ago. We love it, it gave very strong and clean band on Western.
Date published: 2020-03-02
Rated 5 out of 5 by from greatStrong 180kd band was detected by western blotting with SK-BR-3 cells lysate in dilution 1:500
Date published: 2019-08-27
Rated 3 out of 5 by from Antibody is okayGot bands without confusing non-specific binding, but not as clear and defined as I hope it would be.
Date published: 2019-03-04
Rated 4 out of 5 by from Good option for WB and IFNot very strong but positive WB band in human primary cells that don't overexpress the receptor. Also work for IF.
Date published: 2018-11-04
Rated 4 out of 5 by from anti Neu Antibody (3B5) sc-33684anti Neu Antibody (3B5) sc-33684 in IHC stained in green
Date published: 2018-10-19
Rated 4 out of 5 by from works great for WBStrong 180kd band was detected by western blotting with hela cells lysate in dilution 1:1000.
Date published: 2018-05-02
Rated 4 out of 5 by from Good result on mouse tissueGood result at 1/50 with breast tumour mouse tissue
Date published: 2018-03-15
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_35
  • loc_zh_CN, sid_33684, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT
ErbB2/HER2 抗体 (3B5) is rated 4.4 out of 5 by 35.
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_35
  • loc_zh_CN, sid_33684, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 108ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。