Date published: 2025-9-8

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NCAM-L1 抗体 (C-2): sc-514360

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说明书
  • NCAM-L1抗体(C-2)是小鼠单克隆IgG1 κ, 在19篇文献中引用,规格为200 µg/ml
  • 特异性表位图谱分析在human的NCAM-L1的C-terminal细胞质域内的1231-1258氨基酸之间进行
  • 抗-NCAM-L1 抗体 (C-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 NCAM-L1
  • 抗NCAM-L1抗体(C-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NCAM-L1 (C-2): sc-514360免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 NCAM-L1 抗体 (C-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NCAM-L1 抗体 (C-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

NCAM-L1抗体(C-2)是一种IgG1 κ小鼠单克隆NCAM-L1抗体(也称为NCAM-L1抗体或L1CAM抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的NCAM-L1。NCAM-L1抗体(C-2)既可以是非偶联的抗NCAM-L1抗体形式,也可以是多种偶联形式的抗NCAM-L1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞粘附分子是一组密切相关的细胞表面糖蛋白,参与生长过程中的细胞间相互作用,被认为在胚胎发育和发育中起重要作用。神经元细胞粘附分子(NCAM)的表达在各种人类肿瘤中均可观察到,包括神经母细胞瘤、横纹肌肉瘤、威尔姆斯瘤、尤因肉瘤和一些原始髓系恶性肿瘤。NCAM-L1粘附分子(CD171)在神经系统的轴突引导和细胞迁移中起重要作用。NCAM-L1可能通过促进细胞粘附和迁移而有助于肿瘤进展,并且已知它由神经元、神经母细胞瘤和其他恶性肿瘤表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NCAM-L1 抗体 (C-2) 参考文献:

  1. 人类神经细胞粘附分子L1细胞外结构域的错义突变会减少小鼠小脑神经元的神经突起生长。  |  Michelson, P., et al. 2002. Hum Mutat. 20: 481-2. PMID: 12442287
  2. 通过噬菌体展示技术,产生针对小鼠神经细胞粘附分子L1的亲和力成熟的单域抗体。  |  Dong, L., et al. 2003. Biochem Biophys Res Commun. 301: 60-70. PMID: 12535641
  3. 水迷宫学习和前脑神经细胞粘附分子L1的mRNA表达。  |  Venero, C., et al. 2004. J Neurosci Res. 75: 172-181. PMID: 14705138
  4. 神经细胞粘附分子L1是丘脑皮质纤维和皮质丘脑纤维束化和路由化的必要条件。  |  Ohyama, K., et al. 2004. Neurosci Res. 48: 471-5. PMID: 15041201
  5. 神经细胞粘附分子L1的病理错义突变会影响L1基质上的神经突起生长和分支。  |  Cheng, L. and Lemmon, V. 2004. Mol Cell Neurosci. 27: 522-30. PMID: 15555929
  6. Rabex-5蛋白可调节泛素化神经细胞粘附分子L1的内吞运输途径。  |  Aikawa, Y. 2012. J Biol Chem. 287: 32312-23. PMID: 22846990
  7. 针对神经细胞粘附分子L1的Fab片段可促进成年小鼠脊髓损伤后的功能恢复。  |  Loers, G., et al. 2014. Biochem J. 460: 437-46. PMID: 24673421
  8. 神经细胞粘附分子L1样蛋白(CHL1)在胃肠道间质瘤(GIST)中的表达和血清水平及其预后能力。  |  Karstens, KF., et al. 2020. Oncotarget. 11: 1131-1140. PMID: 32284790
  9. 神经细胞粘附分子L1的新功能与X连锁脑积水和MASA综合征有关。  |  Jouet, M., et al. 1995. Am J Hum Genet. 56: 1304-14. PMID: 7762552
  10. 酪蛋白激酶II使神经细胞粘附分子L1磷酸化。  |  Wong, EV., et al. 1996. J Neurochem. 66: 779-86. PMID: 8592152

订购信息

产品名称产品编号规格价格数量收藏夹

NCAM-L1 抗体 (C-2)

sc-514360
200 µg/ml
RMB2377.00

NCAM-L1 (C-2): m-IgG Fc BP-HRP 套装

sc-527891
200 µg Ab; 10 µg BP
RMB2662.00

NCAM-L1 (C-2): m-IgG1 BP-HRP 套装

sc-533264
200 µg Ab; 20 µg BP
RMB2662.00

NCAM-L1 抗体 (C-2) AC

sc-514360 AC
500 µg/ml, 25% agarose
RMB3129.00

NCAM-L1 抗体 (C-2) HRP

sc-514360 HRP
200 µg/ml
RMB2377.00

NCAM-L1 抗体 (C-2) FITC

sc-514360 FITC
200 µg/ml
RMB2482.00

NCAM-L1 抗体 (C-2) PE

sc-514360 PE
200 µg/ml
RMB2580.00

NCAM-L1 抗体 (C-2) Alexa Fluor® 488

sc-514360 AF488
200 µg/ml
RMB2685.00

NCAM-L1 抗体 (C-2) Alexa Fluor® 546

sc-514360 AF546
200 µg/ml
RMB2685.00

NCAM-L1 抗体 (C-2) Alexa Fluor® 594

sc-514360 AF594
200 µg/ml
RMB2685.00

NCAM-L1 抗体 (C-2) Alexa Fluor® 647

sc-514360 AF647
200 µg/ml
RMB2685.00

NCAM-L1 抗体 (C-2) Alexa Fluor® 680

sc-514360 AF680
200 µg/ml
RMB2685.00

NCAM-L1 抗体 (C-2) Alexa Fluor® 790

sc-514360 AF790
200 µg/ml
RMB2685.00

NCAM-L1 (C-2) 中和勝肽

sc-514360 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-514360 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Antibody recognizing the iCD of L1This antibody is able to detect full-length L1 and L1 fragments containing the intracellular domain. Works very well and reliable for detection of L1 in crude homogenates and in subcellular fractions.
Date published: 2023-01-27
Rated 5 out of 5 by from Good antibody for IP, IF and WBThe C2 antibody works well for detection of L1 in brain and cell lysates. Since it is recognizing the intracellular domain (ICD) of L1 it can not only detect full-length L1 but also L1 fragments which contain the ICD. No signals are found in L1-deficient samples.
Date published: 2023-01-27
Rated 5 out of 5 by from Great immunofluorescence membrane and focalGreat immunofluorescence membrane and focal adhesions staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-27
Rated 5 out of 5 by from Nice Western Blot data of NCAMNice Western Blot data of NCAM-L1 expression in IMR-32, SK-N-MC, HeLa, T98G and SH-SY5Y whole cell lysates and human cerebellum tissue extract . -SCBT QC
Date published: 2013-12-13
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NCAM-L1 抗体 (C-2) is rated 5.0 out of 5 by 4.
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