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Nanog 抗体 (A-11): sc-374001

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说明书
  • Nanog抗体(A-11)是小鼠单克隆IgG1 κ, 在20篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的Nanog的C-terminus附近的氨基酸267-301之间
  • 抗-Nanog 抗体 (A-11) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Nanog
  • 抗Nanog抗体(A-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nanog (A-11): sc-374001免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Nanog Antibody (A-11) for WB applications. 的首选辅助检测试剂,这些试剂现与Nanog Antibody (A-11) 打包提供(请参阅下面的订购信息)。

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関連項目

Nanog抗体(A-11)是一种IgG1 κ小鼠单克隆Nanog抗体(也被称作Nanog抗体或Nanog同源框抗体),它通过WB、IP、IF和ELISA检测方法检测小鼠、大鼠和人类来源的Nanog。Nanog抗体(A-11)既可以作为非偶联形式的抗Nanog抗体使用,也可以作为多种偶联形式的抗Nanog抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Nanog(源自"Tir Na Nog",即神话中凯尔特人的永恒青春之地)是一种不同的同源结构域蛋白,指导未分化胚胎干细胞的多能性和分化。Nanog mRNA存在于多能的小鼠和人类细胞系中,但不存在于分化细胞中。人类Nanog蛋白与小鼠蛋白的整体氨基酸同一性为52%,在同源结构域中的同一性为85%。人类Nanog定位于基因位点12p13.31,而小鼠Nanog定位于基因位点6 F2。小鼠胚胎Nanog的表达在囊胚的内细胞团中被检测到。在未分化的NTERA-2 cl.D1胚胎癌细胞系中,通过Northern分析检测到了高水平的人类Nanog表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Nanog 抗体 (A-11)

sc-374001
200 µg/ml
RMB2377.00

Nanog (A-11): m-IgG Fc BP-HRP 套装

sc-527428
200 µg Ab; 10 µg BP
RMB2662.00

Nanog (A-11): m-IgG1 BP-HRP 套装

sc-532801
200 µg Ab; 20 µg BP
RMB2662.00

Nanog 抗体 (A-11) AC

sc-374001 AC
500 µg/ml, 25% agarose
RMB3129.00

Nanog 抗体 (A-11) HRP

sc-374001 HRP
200 µg/ml
RMB2377.00

Nanog 抗体 (A-11) FITC

sc-374001 FITC
200 µg/ml
RMB2482.00

Nanog 抗体 (A-11) PE

sc-374001 PE
200 µg/ml
RMB2580.00

Nanog 抗体 (A-11) Alexa Fluor® 488

sc-374001 AF488
200 µg/ml
RMB2685.00

Nanog 抗体 (A-11) Alexa Fluor® 546

sc-374001 AF546
200 µg/ml
RMB2685.00

Nanog 抗体 (A-11) Alexa Fluor® 594

sc-374001 AF594
200 µg/ml
RMB2685.00

Nanog 抗体 (A-11) Alexa Fluor® 647

sc-374001 AF647
200 µg/ml
RMB2685.00

Nanog 抗体 (A-11) Alexa Fluor® 680

sc-374001 AF680
200 µg/ml
RMB2685.00

Nanog 抗体 (A-11) Alexa Fluor® 790

sc-374001 AF790
200 µg/ml
RMB2685.00

Nanog (A-11) 中和勝肽

sc-374001 P
100 µg/0.5 ml
RMB511.00

What is the recommended secondary reagent to be used with Nanog Antibody (A-11): sc-374001 for Western blot when working with human samples?

Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 7
Date published: 2017-01-18
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Rated 5 out of 5 by from Very good antibodyWe used the antibody to perform Western Blot. It worked perfectly in our samples. I highly recommend it.
Date published: 2024-04-30
Rated 5 out of 5 by from Good AntibodyI have used this Ab for IHC in human pancreas tissue it worked fine
Date published: 2017-08-21
Rated 5 out of 5 by from this antibody worked wellA nice antibody for western blot, bands were clear.
Date published: 2017-08-17
Rated 5 out of 5 by from for WBNanog (A-11) antibody is great, strong and clear, specific band in WB for mouse cells.
Date published: 2017-04-18
Rated 5 out of 5 by from Works well for WBNanog (A-11) works well for Western blot (WB). Recommend NTERA-2 cl.D1 Whole Cell Lysate: sc-364181 as a positive control.
Date published: 2016-12-28
Rated 5 out of 5 by from Used for studying catalyzes the first twoUsed for studying catalyzes the first two steps in lysine degradation
Date published: 2015-05-15
Rated 5 out of 5 by from Nice Western blot analysis of Nanog expressionNice Western blot analysis of Nanog expression in HeLa nuclear extract.
Date published: 2015-05-06
Rated 5 out of 5 by from Published ICC data with chicken primordiaPublished ICC data with chicken primordia germ cells (PGCs) - SCBT Publication Review
Date published: 2013-01-23
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Nanog 抗体 (A-11) is rated 5.0 out of 5 by 8.
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