Date published: 2025-9-9
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Nanog Antibody (5A10) - Western Blotting - Image 286795
Nanog 抗体 (5A10): sc-134218. 对 HeLa (A) 和 Raji (B) 全细胞裂解液以及人卵巢组织提取物 (C) 中 Nanog 表达的 Western 印迹分析.
Nanog 抗体 (5A10): sc-134218. 对 HeLa (A) 和 Raji (B) 全细胞裂解液以及人卵巢组织提取物 (C) 中 Nanog 表达的 Western 印迹分析.

Nanog 抗体 (5A10): sc-134218

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说明书
  • Nanog 抗体 5A10 是小鼠单克隆 IgG2a, 在38篇文献中引用，提供 50 µg/500 µl
  • 与氨基酸1-154的抗原性，对应于human起源的重组Nanog
  • 推荐用于 mouse, rat 和 human 来源的Nanog WB, IP 和 ELISA检测
  • 看 Nanog (1E6C4): sc-293121，可查看有标记的 Nanog 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取Nanog (5A10): sc-134218的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Nanog Antibody (5A10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Nanog Antibody (5A10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Nanog抗体（5A10）是一种IgG2a κ小鼠单克隆Nanog抗体（也被称作Nanog抗体或Nanog同源框抗体），它通过WB、IP和ELISA检测方法检测小鼠、大鼠和人类来源的Nanog。Nanog抗体（5A10）可以作为非偶联形式的抗Nanog抗体使用。Nanog（源自"Tir Na Nog"，即神话中凯尔特人的永恒青春之地）是一种不同的同源结构域蛋白，指导未分化胚胎干细胞的多能性和分化。Nanog mRNA存在于多能的小鼠和人类细胞系中，但不存在于分化细胞中。人类Nanog蛋白与小鼠蛋白的整体氨基酸同一性为52%，在同源结构域中的同一性为85%。人类Nanog定位于基因位点12p13.31，而小鼠Nanog定位于基因位点6 F2。小鼠胚胎Nanog的表达在囊胚的内细胞团中被检测到。在未分化的NTERA-2 cl.D1胚胎癌细胞系中，通过Northern分析检测到了高水平的人类Nanog表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nanog 抗体 (5A10) 参考文献:

  1. 胚胎干细胞多能性维持因子 Nanog 的功能表达克隆。  |  Chambers, I., et al. 2003. Cell. 113: 643-55. PMID: 12787505
  2. 鉴定多能性维持因子 nanog 中两个不同的转录激活结构域  |  Pan, GJ. and Pei, DQ. 2003. Cell Res. 13: 499-502. PMID: 14728807
  3. 人类 STELLAR, NANOG 和 GDF3 基因在多能细胞中表达，并映射到 12p13 染色体上，这是畸胎瘤的一个热点。  |  Clark, AT., et al. 2004. Stem Cells. 22: 169-79. PMID: 14990856
  4. 小鼠和人类多能性促进基因 Nanog 的鉴定, 克隆和表达分析。  |  Hart, AH., et al. 2004. Dev Dyn. 230: 187-98. PMID: 15108323
  5. NANOG 的 11 个女儿。  |  Booth, HA. and Holland, PW. 2004. Genomics. 84: 229-38. PMID: 15233988
  6. 人类 NT2 细胞在神经元分化过程中，Nanog 和 Oct-4 上游区域的 DNA 顺序甲基化。  |  Deb-Rinker, P., et al. 2005. J Biol Chem. 280: 6257-60. PMID: 15615706
  7. 干细胞多能因子 NANOG 在人类胎儿性腺细胞, 睾丸原位癌和生殖细胞肿瘤中均有表达。  |  Hoei-Hansen, CE., et al. 2005. Histopathology. 47: 48-56. PMID: 15982323

订购信息

产品名称产品编号规格价格数量收藏夹

Nanog 抗体 (5A10)

sc-134218
50 µg/500 µl
RMB2377.00

Nanog (5A10): m-IgG Fc BP-HRP 套装

sc-537363
50 µg Ab; 10 µg BP
RMB2662.00

Nanog (5A10): m-IgGκ BP-HRP 套装

sc-534610
50 µg Ab; 40 µg BP
RMB2662.00

Nanog (5A10): m-IgG2a BP-HRP 套装

sc-547991
50 µg Ab; 10 µg BP
RMB2662.00

Is this Nanog (5A10): sc-134218 monoclonal antibody available directly conjugated to HRP?

Asked by: cjMara
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using Nanog (1E6C4): sc-293121, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/nanog-antibody-1e6c4)
Answered by: Technical Support
Date published: 2017-01-30
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Rated 4 out of 5 by from Buon funzionamentoHo provato l' anticorpo primario Nanog (5A10):sc-134218. Lo ho usato per western blot e devo dire che il risultato è stato soddisfacente. Ho usato una concentrazione (1:2000) più bassa di quella suggerita (1:1000) ed ho avuto un buon risultato lo stesso su due membrane su cui avevo corso 30µ e 40 µg di proteine. L’incubazione con il primario è stata fatta ON a 4°C ed ho usato un secondario anti mouse 1:20000. Le bande sono nette e c’è poco aspecifico. Dopo l’incubazione con primario e dopo l’incubazione con secondario ho eseguito 3x10 min di lavaggi con TPBS (PBS+0.1% Tween) ed il blocking è stato fatto per 4 ore con Milk al 5% in TPBS a temperatura ambiente. Ritengo sia un prodotto valido per il mio tipo di ricerca e per il western blot in generale.
Date published: 2020-07-21
Rated 5 out of 5 by from very pleasedThat was a very good antibody, diluted 1:1000 in human ovary tissue , we obtained good results.
Date published: 2017-05-11
Rated 5 out of 5 by from greatClean bands obtained in western blot using human ovary tissue extract.
Date published: 2017-04-17
Rated 5 out of 5 by from Produced publishable WB data on human and mouseWell characterized with publications in the primary literature for WB in human and mouse samples
Date published: 2017-01-11
Rated 5 out of 5 by from WB: strongWB: strong, clean band in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from Positive results in WB with transfected lysatesPositive results in WB with transfected lysates. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Clean bands in western blot analysis of NanogClean bands in western blot analysis of Nanog expression in HeLa and Raji whole cell lysates and human ovary tissue extract. -SCBT QC
Date published: 2013-08-25
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Nanog 抗体 (5A10) is rated 4.9 out of 5 by 7.
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