Date published: 2025-9-6

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N-Myc 抗体 (NCM II 100): sc-56729

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说明书
  • N-Myc抗体(NCM II 100)是小鼠单克隆IgG1 κ, 在34篇文献中引用,规格为200 µg/ml
  • 免疫human物种的重组N-Myc
  • 抗-N-Myc 抗体 (NCM II 100) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 N-myc encoded proteins and their cleavage products
  • 抗N-Myc抗体(NCM II 100)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取N-Myc (NCM II 100): sc-56729免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是N-Myc Antibody (NCM II 100) for WB applications. 的首选辅助检测试剂,这些试剂现与N-Myc Antibody (NCM II 100) 打包提供(请参阅下面的订购信息)。

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関連項目

N-Myc抗体(NCM II 100)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠和人源的N-myc蛋白。N-Myc (NCM II 100) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。N-myc蛋白是Myc家族致癌基因的成员之一,在细胞增殖、分化及凋亡中发挥着关键作用,是癌症生物学中的重要因素。N-myc失调,尤其是基因扩增导致的失调,常见于神经母细胞瘤,其中N-myc的表达水平与疾病进展和预后相关。N-myc蛋白主要定位于细胞核,作为转录因子调节细胞周期进展和生长相关的基因。N-myc的核定位使其能够与特定的DNA序列和其他转录调节因子相互作用,影响可能导致肿瘤发展的细胞过程。了解N-myc的功能和定位对于开发旨在抑制N-myc活性的靶向癌症疗法仍然至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

N-Myc 抗体 (NCM II 100)

sc-56729
200 µg/ml
RMB2377.00

N-Myc (NCM II 100): m-IgG Fc BP-HRP 套装

sc-526887
200 µg Ab; 10 µg BP
RMB2662.00

N-Myc (NCM II 100): m-IgG1 BP-HRP 套装

sc-532260
200 µg Ab; 20 µg BP
RMB2662.00

N-Myc 抗体 (NCM II 100) AC

sc-56729 AC
500 µg/ml, 25% agarose
RMB3129.00

N-Myc 抗体 (NCM II 100) HRP

sc-56729 HRP
200 µg/ml
RMB2377.00

N-Myc 抗体 (NCM II 100) FITC

sc-56729 FITC
200 µg/ml
RMB2482.00

N-Myc 抗体 (NCM II 100) PE

sc-56729 PE
200 µg/ml
RMB2580.00

N-Myc 抗体 (NCM II 100) Alexa Fluor® 488

sc-56729 AF488
200 µg/ml
RMB2685.00

N-Myc 抗体 (NCM II 100) Alexa Fluor® 546

sc-56729 AF546
200 µg/ml
RMB2685.00

N-Myc 抗体 (NCM II 100) Alexa Fluor® 594

sc-56729 AF594
200 µg/ml
RMB2685.00

N-Myc 抗体 (NCM II 100) Alexa Fluor® 647

sc-56729 AF647
200 µg/ml
RMB2685.00

N-Myc 抗体 (NCM II 100) Alexa Fluor® 680

sc-56729 AF680
200 µg/ml
RMB2685.00

N-Myc 抗体 (NCM II 100) Alexa Fluor® 790

sc-56729 AF790
200 µg/ml
RMB2685.00

Which residues does this N-Myc antibody recognizes?

Asked by: gsegat
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody have not yet been determined.
Answered by: Technical Support
Date published: 2020-05-07

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?
Answered by: Technical Service 11
Date published: 2021-01-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-11
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Rated 5 out of 5 by from Working wellwe have tested in cells and it is working in western blot
Date published: 2022-01-12
Rated 3 out of 5 by from Works OKWe used it for IF, and it works OK in neuroblastoma cell lines, which have high endogenous N-Myc protein levels.. Not as good as B4.8.B though.
Date published: 2019-08-14
Rated 5 out of 5 by from Validated MYCN antibody for FFPE IHC useMYCN II 100 antibody was raised against the second exon of MYCN (Proc Natl Acad Sci USA. 1986 Aug;83(16):5929–33.) and used to stain a large cohort of neuroblastomas (Br J Cancer. 2015 Jun 30;113(1):57–63). MYCN is expressed in the skin hair follicles (Developmental Cell. 2005 Aug;9(2):293–303) and in 30% of basal cell carcinomas (Acta Derm Venereol. 2019 Jan 1;99(1):63–71).
Date published: 2019-07-14
Rated 5 out of 5 by from Versatile antibody!The N-Myc Antibody (NCM II 100): sc-56729 works very well for multiple applications including WB, IP, and IF. This antibody has also been cited multiple times for further verification.
Date published: 2017-01-12
Rated 5 out of 5 by from Good WB with 3T3Good WB with 3T3-MycER fibroblasts, ES cells. -SCBT Publication Review
Date published: 2012-08-28
Rated 5 out of 5 by from Used IGRUsed IGR-N-91 cell line in Western Blot -SCBT Publication Review
Date published: 2012-02-04
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N-Myc 抗体 (NCM II 100) is rated 4.7 out of 5 by 6.
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