Date published: 2025-9-7
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Mucin 2/MUC2 Antibody (F-2) - Immunohistochemistry - Image 359495
粘蛋白 2 抗体 (F-2): sc-515032. 免疫过氧化物酶检测福尔马林固定, 石蜡包埋的人小肠组织中的 Mucin 2, 显示鹅口疮细胞的胞浆染色. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
粘蛋白 2 抗体 (F-2): sc-515032. 免疫过氧化物酶检测福尔马林固定, 石蜡包埋的人小肠组织中的 Mucin 2, 显示鹅口疮细胞的胞浆染色. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

Mucin 2/MUC2 抗体 (F-2): sc-515032

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说明书
  • Mucin 2/MUC2 抗体 F-2 是小鼠单克隆 IgG1 κ，Mucin 2/MUC2抗体, 在48篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列4880-5179位于human物种的Mucin 2的C-terminus
  • Mucin 2/MUC2 抗体 (F-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Mucin 2
  • 抗Mucin 2/MUC2抗体（F-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mucin 2/MUC2 (F-2): sc-515032的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Mucin 2/MUC2 抗体 (F-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Mucin 2/MUC2 抗体 (F-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Mucin 抗体 包括 Mucin 1, Mucin 12, Mucin 13, Mucin 15, Mucin 16, Mucin 2, Mucin 3, Mucin 4, Mucin 5AC和Mucin 5B

Mucin 2抗体（F-2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测人源Mucin 2蛋白。抗粘蛋白2抗体（F-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。粘蛋白2，也称为低聚粘液/凝胶形成粘蛋白，在肠道和呼吸道形成保护性粘液层方面起着至关重要的作用，这对于维持上皮完整性并促进清除病原体和碎屑至关重要。粘蛋白2的结构特点是高度糖基化和可变数量串联重复序列（VNTR）的独特排列，这有助于形成凝胶和捕获水分，从而形成粘性屏障，保护底层组织。粘蛋白2的表达和功能改变与多种胃肠道疾病有关，包括炎症性肠病和结肠直肠癌，这凸显了粘蛋白2在健康和疾病中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mucin 2/MUC2 抗体 (F-2) 参考文献:

  1. [内毒素血症期间血小板活化因子受体拮抗剂对幼鼠肠道黏蛋白-2的影响]。  |  Wang, LJ. and Sun, M. 2008. Zhonghua Er Ke Za Zhi. 46: 680-3. PMID: 19099858
  2. 宫内生长受限改变大鼠出生后的结肠屏障成熟。  |  Fança-Berthon, P., et al. 2009. Pediatr Res. 66: 47-52. PMID: 19287349
  3. 低聚半乳糖可通过调节杯状细胞直接增强肠道屏障功能。  |  Bhatia, S., et al. 2015. Mol Nutr Food Res. 59: 566-73. PMID: 25421108
  4. 通过在肠道上皮细胞中产生表达 GFP 的转基因鹌鹑，确定 MUC2 启动子是肠道基因表达的强启动子。  |  Woodfint, RM., et al. 2017. Int J Mol Sci. 18: PMID: 28106824
  5. 血清淀粉样蛋白A3蛋白的N端区域激活NF-κB并上调小鼠结肠上皮细胞中MUC2粘蛋白mRNA的表达。  |  Tashiro, M., et al. 2017. PLoS One. 12: e0181796. PMID: 28738073
  6. 宿主金属蛋白酶Meprin β的粘液分离需要其非活性前体的脱落，而后者被病原蛋白酶RgpB所破坏。  |  Wichert, R., et al. 2017. Cell Rep. 21: 2090-2103. PMID: 29166602
  7. 将人类iPS细胞衍生的肠细胞作为评估人类肠道粘膜损伤的新型体外模型。  |  Kondo, S., et al. 2018. Inflamm Res. 67: 975-984. PMID: 30317465
  8. 胆汁酸通过 FXR/NF-κB 信号通路上调 CDX2 和 MUC2 的表达，从而促进胃肠化生。  |  Yu, JH., et al. 2019. Int J Oncol. 54: 879-892. PMID: 30747230
  9. 枯草芽孢杆菌 RZ001 通过抑制 Notch 信号通路和激活 ATOH-1 改善肠道完整性并缓解结肠炎。  |  Li, Y., et al. 2020. Pathog Dis. 78: PMID: 32166323
  10. 电针通过增加水回避应激小鼠中核苷酸结合多聚体蛋白样受体家族吡啶结构域6的表达来缓解肠易激综合征。  |  Song, Y., et al. 2020. J Tradit Chin Med. 40: 407-413. PMID: 32506854

订购信息

产品名称产品编号规格价格数量收藏夹

Mucin 2/MUC2 抗体 (F-2)

sc-515032
200 µg/ml
$316.00

Mucin 2/MUC2 (F-2): m-IgG Fc BP-HRP 套装

sc-527969
200 µg Ab; 10 µg BP
$354.00

Mucin 2/MUC2 (F-2): m-IgG1 BP-HRP 套装

sc-533342
200 µg Ab; 20 µg BP
$354.00

Mucin 2/MUC2 抗体 (F-2) AC

sc-515032 AC
500 µg/ml, 25% agarose
$416.00

Mucin 2/MUC2 抗体 (F-2) HRP

sc-515032 HRP
200 µg/ml
$316.00

Mucin 2/MUC2 抗体 (F-2) FITC

sc-515032 FITC
200 µg/ml
$330.00

Mucin 2/MUC2 抗体 (F-2) PE

sc-515032 PE
200 µg/ml
$343.00

Mucin 2/MUC2 抗体 (F-2) Alexa Fluor® 488

sc-515032 AF488
200 µg/ml
$357.00

Mucin 2/MUC2 抗体 (F-2) Alexa Fluor® 546

sc-515032 AF546
200 µg/ml
$357.00

Mucin 2/MUC2 抗体 (F-2) Alexa Fluor® 594

sc-515032 AF594
200 µg/ml
$357.00

Mucin 2/MUC2 抗体 (F-2) Alexa Fluor® 647

sc-515032 AF647
200 µg/ml
$357.00

Mucin 2/MUC2 抗体 (F-2) Alexa Fluor® 680

sc-515032 AF680
200 µg/ml
$357.00

Mucin 2/MUC2 抗体 (F-2) Alexa Fluor® 790

sc-515032 AF790
200 µg/ml
$357.00

I am from Weill Cornell Medicine. Is there any discount for Mucin 2/MUC2 Antibody (F-2): sc-515032? Thanks

Asked by: xiaohua duan
Thank you for contacting Santa Cruz Biotechnology . To request a discount please email [email protected]
Answered by: BlakeJ
Date published: 2023-12-29

What is the host species of sc-515032 ?

Asked by: soojeong9482
Thank you for your question. Anti-Mucin 2/MUC2 Antibody (F-2) is a mouse monoclonal IgG1 antibody.
Answered by: Tech Support Europe
Date published: 2021-08-26

We have been unsuccessful at obtaining Muc2 bands in HT29 MTX E12 lysates (goblet cells) which should be producing high levels. What is your recommendation for the lysis as well as what type of gel, since the molecular weight is potentially 600kD (dimer)?

Asked by: Viola L
Thank you for your question. We would recommend using a low percentage gel such as 4-6% for this very large protein. We use RIPA lysis buffer in our lysate preparations, and Electrophoresis Sample Buffer for our Western blot.
Answered by: Tech Service
Date published: 2019-10-03

 What's antigen retrieval condition for this antibody (Mucin 2 (F-2): sc-515032) in staining formalin fixed, paraffin-embedded human small intestine tissue ? Thanks!

Asked by: Henson
Thank you for your question. We recommend using 10 mM sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this Mucin 2 Antibody (F-2): sc-515032. A link to our complete recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-08-21

What is the size expected when performed a western blot analysis of Mucin 2 expression in mouse?

Asked by: srocha
Thank you for your inquiry. The expected molecular weight for Mucin 2 monomer is ~300kDa, with the possibility of forming a dimer (which is calculation to be ~520-600kDa). If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-03-21

What is the total volume provided of Mucin 2 (F-2): sc-515032 monoclonal antibody?

Asked by: jenni
Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 5 out of 5 by from Best qualityGreat product, worked well in our laboratory research experiments
Date published: 2024-09-05
Rated 5 out of 5 by from Great staining on FFPE bowel sectionsProduced great staining within goblet cell granules for fluorescent microscopy with little background. Appears to be specifically staining the glycosylated forms of Muc2. It never shows perinuclear staining. Clone CCP58 for MUC2 antibodies should show that better.
Date published: 2019-02-23
Rated 3 out of 5 by from IF of MUC2 antibody (F-2) in mouse colonI haven't gotten positive staining of MUC2 in the colon of WT mice. I ordered the free sample, the unconjugated mouse monoclonal Mucin 2 IgG1 Antibody (F-2) antibody. I tried three different kinds of secondary antibodies: Goat anti mouse IgG (H+L), A21422, Invitrogen, donkey anti mouse IgG (H+L), A21202, Invitrogen, and goat anti mouse IgG1, A21127, Invitrogen.
Date published: 2018-04-16
Rated 1 out of 5 by from 效果不好申请的MUC2（F2）抗体小样，做了肠组织免疫荧光，结果不好，没有染出来,不知道那些能染出来是怎末做的?
Date published: 2018-03-02
Rated 1 out of 5 by from WB for mouse intestineDoes not work via western blot for mouse intestine cells.
Date published: 2018-02-21
Rated 1 out of 5 by from Did not workDid not work in PFA fixed intestinal organoid samples from human or mouse. The previous antibody which was discontinued worked amazing every time now I don't know what will be a good replacement.
Date published: 2017-12-20
Rated 1 out of 5 by from It does not work in my mouse colonIt does not work in my mouse colon, I used the Mucin 2 Antibody (F-2) Alexa Fluor® 647.
Date published: 2017-10-24
Rated 1 out of 5 by from ICC: doesn't workWe received a test sample as a replacement for SC-15334 which is discontinued. We stained PFA-fixed mouse intestinal organoids as wholemounts - this antibody doesn't work, it stains effectively the remainders of Matrigel.
Date published: 2017-06-10
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Mucin 2/MUC2 抗体 (F-2) is rated 2.9 out of 5 by 11.
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