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Mucin 2/MUC2 Antibody (Ccp58) - Immunohistochemistry - Image 160065
粘蛋白 2 抗体 (Ccp58): sc-7314. 福尔马林固定, 石蜡包埋的正常人结肠组织的免疫过氧化物酶染色, 显示分泌型 Mucin 2 染色.
粘蛋白 2 抗体 (Ccp58): sc-7314. 福尔马林固定, 石蜡包埋的正常人结肠组织的免疫过氧化物酶染色, 显示分泌型 Mucin 2 染色.

Mucin 2/MUC2 抗体 (Ccp58): sc-7314

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寫評論提問

说明书
  • Mucin 2/MUC2抗体(Ccp58)是小鼠单克隆IgG1 κ, 在87篇文献中引用，规格为200 µg/ml
  • 与编码human的Mucin 2的5个串联重复的合成肽杂交
  • 抗-Mucin 2/MUC2 抗体 (Ccp58) 推荐用于 IF 和 IHC(P)，检测mouse, rat 和human 来源的 Mucin 2
  • 也可与Alexa Fluor® 488 (sc-7314 AF488)或Alexa Fluor® 594 (sc-7314 AF594)结合使用，200 µg/ml，用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mucin 2/MUC2 (Ccp58): sc-7314的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Mucin 2/MUC2 Antibody (Ccp58) for IHC(P) applications. 的首选辅助检测试剂，这些试剂现与Mucin 2/MUC2 Antibody (Ccp58) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Mucin抗体，包括Mucin 1, Mucin 12, Mucin 13, Mucin 15, Mucin 16, Mucin 2, Mucin 3, Mucin 4, Mucin 5AC, Mucin 5B, Mucin 6, Mucin 7和Epithelial sialomucin

Mucin 2/MUC2抗体（Ccp58）是一种IgG1 κ小鼠单克隆Mucin 2抗体，可通过IF和IHC(P)检测小鼠、大鼠和人源的Mucin 2。Mucin 2/MUC2抗体（Ccp58）既可以是偶联的，也可以是非偶联的抗Mucin 2抗体形式。粘蛋白是一类高度糖基化的分泌蛋白，其基本结构由可变数量的串联重复序列（VNTRs）组成，这些重复序列分别由60个碱基对（Mucin 1）、69个碱基对（Mucin 2）和51个碱基对（Mucin 3）编码。重复次数高度多态，不同等位基因之间有所不同。Mucin 1蛋白除了分泌形式外，还以I型膜蛋白的形式表达。Mucin 1在上皮性肿瘤包括乳腺癌中异常表达。Mucin 2覆盖肠道和气道上皮，与结肠肿瘤相关。Mucin 3是各种粘液凝胶的主要成分，在正常细胞和肿瘤细胞中广泛表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mucin 2/MUC2 抗体 (Ccp58) 参考文献:

  1. N-连接寡糖在肠粘蛋白 Muc2 的二硫化物依赖性二聚化中发挥作用。  |  Bell, SL., et al. 2003. Biochem J. 373: 893-900. PMID: 12744721
  2. 人类 MUC2 肠粘蛋白在其中心重复区的上游和下游都有富含半胱氨酸的亚域。  |  Gum, JR., et al. 1992. J Biol Chem. 267: 21375-83. PMID: 1400449
  3. 人类 MUC2 粘蛋白基因在胃肠癌细胞系中受 Cdx 同源域蛋白的转录调控。  |  Mesquita, P., et al. 2003. J Biol Chem. 278: 51549-56. PMID: 14525978
  4. 小鼠 Muc2 粘蛋白基因在肠细胞中受锌指 GATA-4 转录因子的转录调控。  |  van der Sluis, M., et al. 2004. Biochem Biophys Res Commun. 325: 952-60. PMID: 15541382
  5. 一种新型人类肠粘蛋白基因的 cDNAs 分子克隆。  |  Gum, JR., et al. 1990. Biochem Biophys Res Commun. 171: 407-15. PMID: 2393399
  6. 人胰腺肿瘤粘蛋白 cDNA 的克隆和测序。  |  Lan, MS., et al. 1990. J Biol Chem. 265: 15294-9. PMID: 2394722
  7. 人类 DF3 乳腺癌相关抗原编码 cDNA 的分离和测序。  |  Siddiqui, J., et al. 1988. Proc Natl Acad Sci U S A. 85: 2320-3. PMID: 2895474
  8. 粘蛋白-2基因敲除是肠道上皮细胞间连接缺陷、线粒体损伤和ATP耗竭的模型。  |  Borisova, MA., et al. 2020. Sci Rep. 10: 21135. PMID: 33273633
  9. 小鼠中粘蛋白2的缺失可诱发结肠炎，并伴有代谢异常。  |  Ye, J., et al. 2021. Am J Physiol Gastrointest Liver Physiol. 320: G791-G803. PMID: 33728986
  10. MUC2和相关细菌因子：溃疡性结肠炎的治疗靶点。  |  Yao, D., et al. 2021. EBioMedicine. 74: 103751. PMID: 34902790
  11. 溃疡性结肠炎中的粘蛋白表达谱：对组织学粘膜愈合的新见解。  |  Leoncini, G., et al. 2024. Int J Mol Sci. 25: PMID: 38339134
  12. DF3/MUC1 乳腺癌抗原与 Grb2 和 Sos/Ras 交换蛋白的关联。  |  Pandey, P., et al. 1995. Cancer Res. 55: 4000-3. PMID: 7664271

订购信息

产品名称产品编号规格价格数量收藏夹

Mucin 2/MUC2 抗体 (Ccp58)

sc-7314
200 µg/ml
RMB2377.00

Mucin 2/MUC2 (Ccp58): m-IgG Fc BP-HRP 套装

sc-526497
200 µg Ab; 10 µg BP
RMB2662.00

Mucin 2/MUC2 (Ccp58): m-IgG1 BP-HRP 套装

sc-531870
200 µg Ab; 20 µg BP
RMB2662.00

Mucin 2/MUC2 抗体 (Ccp58) Alexa Fluor® 488

sc-7314 AF488
200 µg/ml
RMB2685.00

Mucin 2/MUC2 抗体 (Ccp58) Alexa Fluor® 546

sc-7314 AF546
200 µg/ml
RMB2685.00

Mucin 2/MUC2 抗体 (Ccp58) Alexa Fluor® 594

sc-7314 AF594
200 µg/ml
RMB2685.00

Mucin 2/MUC2 抗体 (Ccp58) Alexa Fluor® 680

sc-7314 AF680
200 µg/ml
RMB2685.00

Mucin 2/MUC2 抗体 (Ccp58) Alexa Fluor® 790

sc-7314 AF790
200 µg/ml
RMB2685.00

Does this MUC2 antibody have cross-reactivity with MUC1 ?

Asked by: jm123
Thank you for your question. The antibody has been known to only detect MUC2. https://www.uniprot.org/uniprotkb/Q02817/entry#sequences
Answered by: BlakeJ
Date published: 2024-04-15

i received sc-7314 at room temperature though it says to store at 4C. Will antibody integrity be compromised? also- do you have a CoA for this item? Thank you.

Asked by: jujuB
Some of our antibodies ship out ambient. They can last up to 5 days out of storage. We do not have CoAs for our antibodies.
Answered by: Technical Service
Date published: 2023-12-18

I tried using this antibody for IF at a 1:50 and 1:100 dilution in 5% BSA to stain for MUC-2 secreted by HT29-MTX cells but it did not work well.  Is there an antibody that is better suited for this application using HT29-MTX cells?

Asked by: isramire
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.”
Answered by: Blake J
Date published: 2021-11-17

The Rabbit polyclonal muc2 primary antibody H-300 Santa Cruz technologies was been discontinued. Is there an alternative MUC2 antibody that can be used to stain mouse muc2 for IF. What would be the Alexa488 secondary antibody to use?

Asked by: Anonymous
Yes, this antibody sc-7314 should be suitable for IF on mouse tissues. Please contact technical support for further assistance.
Answered by: Tech Service 1
Date published: 2020-11-16

What concentration do you recommend for this antibody for mouse detection via IF and IHC?

Asked by: crrivet
Thank you for your question. The recommended starting dilution is 1:50 (range 1:50-1:500).
Answered by: Technical Support
Date published: 2019-09-27

Will this antibody work in a Sandwich Elisa?

Asked by: KathrinR
Thank you for your question. We have not tested this antibody in an ELISA and so this is not a recommended application at this time.
Answered by: Technical Support
Date published: 2019-04-26

This might be a novice question, however, shouldn't a primary antibody never be from the same host as the target species? Nonetheless, you suggest using this mouse monoclonal antibody to detect mucin 2 in mice. Could you elaborate how this works?

Asked by: Mwolter
Thank you for your question. There is no problem with using a mouse monoclonal antibody for detection of the corresponding protein in mouse cells or tissues. The main concern would be that your secondary antibody (anti-mouse IgG) may cross react non-specifically with endogenous mouse IgG expressed in your samples. This is only really an issue if your samples are rich in endogenous IgG. In that case, we would recommend optimizing your secondary antibody, blocking and incubation conditions. Alternatively, you can always use a directly conjugated primary antibody and avoid using any secondary antibody for detection.
Answered by: Technical Support
Date published: 2019-04-13

Will this antibody cross-react in my zebrafish samples?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 3 out of 5 by from It works well in WBWe tested this Ab by WB at 1:1000 dilution. Strong band and no background. We'll also test it in IF on Human Intestinal Colonoids.
Date published: 2023-03-22
Rated 1 out of 5 by from do not work on IHCdo not work on IHC-DAB and IHC-IF in mouse small intestine and large intestine. dilution 1:50.
Date published: 2020-08-17
Rated 1 out of 5 by from Does not stain goblet cells in murine intestine IFWe purchased two lots of this antibody and they do not stain for Mucin2, instead they appear to stain extracellular matrix or some other protein in the basal lamina propria (attached). We believe this is lot specific (attached), however customer service has chosen to ignore this after we politely brought it to their attention. We do not recommend purchasing this antibody, and are disappointed but not surprised by santa cruz's response to our concerns. We are however amused by the staff 5-star ratings of this antibody, fair play.
Date published: 2019-11-14
Rated 4 out of 5 by from 使用方便，功能齐全我在一个月前购买了mucin-2 此产品，并用于黏液层免疫组化染色，实验效果显著,这个产品使用方便，且抗体效价很高。
Date published: 2019-06-08
Rated 1 out of 5 by from No effect on mouse colon IF stainingNo effect on colonic IF staining in mice, concentration 1:100
Date published: 2019-05-11
Rated 1 out of 5 by from Mouse small intestine tissueDoesn't work for mouse small intestine tissue via IF on cryosections & IF on mouse small intestinal organoids.
Date published: 2018-07-08
Rated 5 out of 5 by from strong bandNo non-specific binding and background ,just strong band,I am not recommend use Tween-20 when blocking the membrane,it may cause background.
Date published: 2017-07-01
Rated 5 out of 5 by from specific stainingWe use Mucin 2 antibody by Immunohistochemistry of human tonsil tissue stained with Anti-Mucin 2 antibody , we got the specific staining.
Date published: 2017-05-28
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Mucin 2/MUC2 抗体 (Ccp58) is rated 3.8 out of 5 by 16.
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