Date published: 2025-9-8

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mtTFA 抗体 (C-9): sc-376672

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说明书
  • mtTFA抗体(C-9)是小鼠单克隆IgG2a κ, 在29篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的mtTFA的C-terminus附近的氨基酸207-241之间
  • mtTFA 抗体 (C-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 mtTFA
  • 抗mtTFA抗体(C-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取mtTFA (C-9): sc-376672免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是mtTFA Antibody (C-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与mtTFA Antibody (C-9) 打包提供(请参阅下面的订购信息)。

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関連項目

mtTFA抗体(C-9)是一种小鼠单克隆IgG2b kappa轻链抗体,专门针对人类mtTFA C末端附近氨基酸207-241之间的表位图谱,通过蛋白质印迹(WB)、免疫 沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)。mtTFA,也称为线粒体转录因子A、TFAM或TCF6,是一种核编码蛋白,被导入线粒体,在那里与线粒体DNA(mtDNA)结合,对线粒体DNA的复制、转录和维护至关重要。作为高迁移率组(HMG)蛋白家族的一员,mtTFA具有DNA结合结构域,能够弯曲并包裹mtDNA,从而促进转录和复制过程的启动。mtTFA在线粒体中的定位至关重要,因为mtTFA直接影响线粒体基因的表达和生物合成,而线粒体的正常功能和细胞能量产生离不开基因的表达和生物合成。mtTFA的表达或功能异常会导致线粒体功能障碍,并引发各种线粒体疾病,表现为肌肉无力、神经退行性疾病和代谢问题。在精子发生过程中,mtTFA水平下降会导致mtDNA拷贝数减少,从而可能阻止父源mtDNA的传递。了解mtTFA在线粒体健康中的作用对于研究衰老、代谢性疾病和线粒体病理非常重要。抗mtTFA抗体(C-9)有非结合型和多种结合型,包括琼脂糖、 辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)以及各种Alexa Fluor®偶联物,为涉及线粒体功能和相关疾病的各种研究应用提供了多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

mtTFA 抗体 (C-9) 参考文献:

  1. 一名携带单个 mtDNA 缺失的患者的 H-Tfam 异常。  |  Tessa, A., et al. 2000. Funct Neurol. 15: 211-4. PMID: 11213524
  2. 线粒体脑肌病患者骨骼肌中人类线粒体转录因子 A 的异常水平。  |  Siciliano, G., et al. 2000. Neurol Sci. 21: S985-7. PMID: 11382203
  3. 热休克蛋白22通过AMPK-PGC1α信号通路调节NRF1/TFAM依赖的线粒体生物发生和DRP1引发的线粒体凋亡,减轻大鼠蛛网膜下腔出血的早期脑损伤。  |  Choi, YS., et al. 2001. Diabetes Res Clin Pract. 54 Suppl 2: S3-9. PMID: 11733104
  4. 黄芪皂苷IV通过上调Nrf2-ARE/TFAM信号通路改善线粒体功能障碍,从而减轻糖尿病肾病中的足细胞凋亡。  |  Lee, H., et al. 2017. Mol Med Rep. 15: 3781-3786. PMID: 28440425
  5. METTL3诱导的IGFBP3表观遗传促进可增强心脏成纤维细胞的活化和纤维化。  |  Araujo, LF., et al. 2018. Sci Rep. 8: 14190. PMID: 30242167
  6. IGFBP3通过调节晚期前列腺癌中的EGFR信号传导,促进对奥拉帕尼的耐药性。  |  Fan, H., et al. 2021. Redox Biol. 40: 101856. PMID: 33472123
  7. 线粒体转录因子A(TFAM)在体外具有5'-脱氧核糖磷酸裂解酶活性。  |  Lu, T., et al. 2023. J Immunother Cancer. 11: PMID: 36858460
  8. WSB2的表达升高会降解p53并激活IGFBP3-AKT-mTOR依赖性通路,从而促进肝癌的发生。  |  Shen, Q., et al. 2023. Free Radic Biol Med. 203: 45-57. PMID: 37030337
  9. ID1和IGFBP3:在细胞衰老、心脏发育、血管生成和癌症诊断中的作用。  |  Zhao, W., et al. 2024. DNA Repair (Amst). 137: 103666. PMID: 38492429
  10. 线粒体转录因子 A (TCF6), 单链 DNA 结合蛋白 (SSBP) 和内切酶 G (ENDOG) 这三个参与线粒体生物发生的人类看家基因的染色体定位。  |  Tiranti, V., et al. 1995. Genomics. 25: 559-64. PMID: 7789991
  11. 一个睾丸特异基因,编码位于伸长精子中的核高流动性组盒蛋白。  |  Boissonneault, G. and Lau, YF. 1993. Mol Cell Biol. 13: 4323-30. PMID: 8321234
  12. 单个小鼠基因编码线粒体转录因子 A 和睾丸特异性核 HMG-box 蛋白。  |  Larsson, NG., et al. 1996. Nat Genet. 13: 296-302. PMID: 8673128

订购信息

产品名称产品编号规格价格数量收藏夹

mtTFA 抗体 (C-9)

sc-376672
200 µg/ml
RMB2377.00

mtTFA (C-9): m-IgG Fc BP-HRP 套装

sc-526044
200 µg Ab; 10 µg BP
RMB2662.00

mtTFA (C-9): m-IgG2a BP-HRP 套装

sc-546490
200 µg Ab; 10 µg BP
RMB2662.00

mtTFA 抗体 (C-9) AC

sc-376672 AC
500 µg/ml, 25% agarose
RMB3129.00

mtTFA 抗体 (C-9) HRP

sc-376672 HRP
200 µg/ml
RMB2377.00

mtTFA 抗体 (C-9) FITC

sc-376672 FITC
200 µg/ml
RMB2482.00

mtTFA 抗体 (C-9) PE

sc-376672 PE
200 µg/ml
RMB2580.00

mtTFA 抗体 (C-9) Alexa Fluor® 488

sc-376672 AF488
200 µg/ml
RMB2685.00

mtTFA 抗体 (C-9) Alexa Fluor® 546

sc-376672 AF546
200 µg/ml
RMB2685.00

mtTFA 抗体 (C-9) Alexa Fluor® 594

sc-376672 AF594
200 µg/ml
RMB2685.00

mtTFA 抗体 (C-9) Alexa Fluor® 647

sc-376672 AF647
200 µg/ml
RMB2685.00

mtTFA 抗体 (C-9) Alexa Fluor® 680

sc-376672 AF680
200 µg/ml
RMB2685.00

mtTFA 抗体 (C-9) Alexa Fluor® 790

sc-376672 AF790
200 µg/ml
RMB2685.00

mtTFA (C-9) 中和勝肽

sc-376672 P
100 µg/0.5 ml
RMB511.00

this type antibodity could used for mouse

Asked by: Wmm123
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What application is the blocking peptide sc-376672 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 9
Date published: 2017-01-17
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Rated 5 out of 5 by from 功能很出色功能很出色,特异性很强,染出来的片子很漂亮,价格实惠,给老板省钱了。非常满意。以后还会尝试使用这家的其他抗体。
Date published: 2024-04-22
Rated 4 out of 5 by from Very solid antibodyThis antibody was used in western blotting application using lysates of cultured human osteosarcoma cells 143B. The antibody produced a strong signal with minimal background and cross-reactivity.
Date published: 2022-07-31
Rated 3 out of 5 by from WBで使用しました。マウスの脳組織でmtTFAをWBで検出しました。条件によってはバンドの検出が難しかったでので、条件の工夫が必要でした。
Date published: 2020-08-05
Rated 5 out of 5 by from Very good antibody!Used for WB at 1:500 dilution. It works very well!
Date published: 2019-10-26
Rated 5 out of 5 by from Excellent Antibody !!I tried the antibody on Human samples. Works well and clear bands at 25kDA
Date published: 2017-10-27
Rated 1 out of 5 by from Mouse macrophagesI tried this antibody on mouse macrophages and it didn't work. I realized later on this works for human samples. I would appreciate if I can get a free sample of sc-166965 antibody. if this antibody works we will definitely order this antibody. Thanks, Harvinder Talwar
Date published: 2017-09-05
Rated 5 out of 5 by from Great antibody!To detect mtTFA in human CD4 T cells, 1:500 in WB, works great!
Date published: 2017-06-27
Rated 5 out of 5 by from Excellent WB useUsed to detect TFA in HeLa cells, got strong WB band.
Date published: 2017-06-08
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mtTFA 抗体 (C-9) is rated 4.5 out of 5 by 21.
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