Date published: 2025-9-8
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mtTFA Antibody (F-6) - Western Blotting - Image 392750
mtTFA 抗体 (F-6) Alexa Fluor® 488: sc-166965 AF488. RAW 264. 7 (A) 和 A-673 (B) 全细胞裂解液中 mtTFA 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.
mtTFA 抗体 (F-6) Alexa Fluor® 488: sc-166965 AF488. RAW 264. 7 (A) 和 A-673 (B) 全细胞裂解液中 mtTFA 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.

mtTFA 抗体 (F-6): sc-166965

4.1(22)
寫評論提問

说明书
  • mtTFA 抗体 F-6 是小鼠单克隆 IgG2b κ，mtTFA抗体, 在85篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的mtTFA的内部区域内的氨基酸198-214之间
  • mtTFA 抗体 (F-6) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 mtTFA
  • 抗mtTFA抗体（F-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-166965 X, 200 µg/0.1 ml)
  • 关于如何获取mtTFA (F-6): sc-166965的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是mtTFA Antibody (F-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与mtTFA Antibody (F-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

mtTFA抗体（F-6）是一种IgG2b κ小鼠单克隆mtTFA抗体（也称为TFAM抗体、MtTf1抗体、TCF6抗体或TCF6L2抗体），可通过WB、IP、IF和ELISA检测来自小鼠、大鼠和人类的mtTFA蛋白。mtTFA抗体（F-6）既有非偶联型抗mtTFA抗体形式，也有多种偶联型抗mtTFA抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。mtTFA（线粒体转录因子A），也称为mtTF1、TFAM、TCF6（转录因子6样1）、TCF6L2和tsHMG，是一种核编码基因产物，被导入到线粒体中。mtTFA在线粒体生物发生的许多方面都是必需的，包括线粒体DNA（mtDNA）的复制和转录。在小鼠中，睾丸特异性的mtTFA缺少线粒体靶向序列，存在于细胞核中而非线粒体中。这种形式的mtTFA主要位于伸长精子的细胞核中，可能参与单倍体雄性基因组基因表达的调控。在小鼠和人类的精子发生过程中，mtTFA蛋白水平和mtDNA拷贝数均有所减少。这些特征可能为防止父系mtDNA传递的机制之一。mtTFA与人类线粒体紊乱有关，其特点为眼肌病、运动不耐受和肌肉萎缩。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

mtTFA 抗体 (F-6)

sc-166965
200 µg/ml
RMB2377.00

mtTFA (F-6): m-IgGκ BP-HRP 套装

sc-521811
200 µg Ab, 40 µg BP
RMB2662.00

mtTFA (F-6): m-IgG2b BP-HRP 套装

sc-548971
200 µg Ab; 10 µg BP
RMB2662.00

mtTFA 抗体 (F-6) X

sc-166965 X
200 µg/0.1 ml
RMB2377.00

mtTFA 抗体 (F-6) AC

sc-166965 AC
500 µg/ml, 25% agarose
RMB3129.00

mtTFA 抗体 (F-6) HRP

sc-166965 HRP
200 µg/ml
RMB2377.00

mtTFA 抗体 (F-6) FITC

sc-166965 FITC
200 µg/ml
RMB2482.00

mtTFA 抗体 (F-6) PE

sc-166965 PE
200 µg/ml
RMB2580.00

mtTFA 抗体 (F-6) Alexa Fluor® 488

sc-166965 AF488
200 µg/ml
RMB2685.00

mtTFA 抗体 (F-6) Alexa Fluor® 546

sc-166965 AF546
200 µg/ml
RMB2685.00

mtTFA 抗体 (F-6) Alexa Fluor® 594

sc-166965 AF594
200 µg/ml
RMB2685.00

mtTFA 抗体 (F-6) Alexa Fluor® 647

sc-166965 AF647
200 µg/ml
RMB2685.00

mtTFA 抗体 (F-6) Alexa Fluor® 680

sc-166965 AF680
200 µg/ml
RMB2685.00

mtTFA 抗体 (F-6) Alexa Fluor® 790

sc-166965 AF790
200 µg/ml
RMB2685.00

mtTFA (F-6) 中和勝肽

sc-166965 P
100 µg/0.5 ml
RMB511.00

如何获得10微的试用小样

Asked by: 冰凌澜心
感谢您的提问。目前我们可以提供该抗体的10 µg 试用装，详情请咨询我们的技术服务部门。亚洲技术服务电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。Thank you for your question. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department to request a sample. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21

Has anyone used this antibody to stain cells via intracellular staining and run them on flow cytometer? If yes, any insight on how it works there?

Asked by: ImmunoGuy
Thank you for your question. We have not yet tested this antibody for that application, and are not aware of any publications citing use of the antibody for flow cytometry at this time. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-11-15

When should the TransCruz reagent sc-166965 X be used instead of the standard form sc-166965?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 9
Date published: 2017-01-17
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Rated 1 out of 5 by from Strong band at 80Kda in mouse cerebellumWB in mouse cerebellum revealed no band at 25kda, 2 weak bands at around 35kda and a very strong band at 80kda. This was the first antibody incubation in the membrane depicted. 1:1000 dilution in 5% BSA in TBS-T Any advice?
Date published: 2023-10-18
Rated 3 out of 5 by from TFAM western and immunofluorescenceI got this antibody few months. glad it is kind of working. except non specific band at 37kD
Date published: 2023-03-28
Rated 5 out of 5 by from Excellent functionI bought this product. It's very easy to use. I recommend you to buy this product.
Date published: 2023-02-01
Rated 5 out of 5 by from It's excellent.The samples were obtained from rat brain. The primary antibody works well.
Date published: 2021-07-21
Rated 5 out of 5 by from great for WB in mouse brainTested for western blot in mouse brain at 1:1000 dilution in 5% milk...got 1 clear band at 28kDa!
Date published: 2021-05-05
Rated 3 out of 5 by from WBで使用マウスの脳組織でmtTFAをWBで検出しました。条件によってはバンドの検出が難しかったでので、条件の工夫が必要でした。
Date published: 2020-08-05
Rated 4 out of 5 by from goodSingle band detected at predicted MW on western analysis
Date published: 2020-02-06
Rated 5 out of 5 by from Clean BandClean Band, good quality. Recommend to other users.
Date published: 2018-10-12
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mtTFA 抗体 (F-6) is rated 4.1 out of 5 by 22.
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