Date published: 2025-9-7

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MPO light chain/Myeloperoxidase 抗体 (A-5): sc-365436

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说明书
  • MPO light chain/Myeloperoxidase 抗体 A-5 是小鼠单克隆 IgG1 κ,MPO light chain/Myeloperoxidase抗体, 在4篇文献中引用,规格为200 µg/ml
  • 针对human起源的MPO内部区域内的氨基酸165-278进行
  • MPO light chain/Myeloperoxidase 抗体 (A-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 MPO light chain
  • 抗MPO light chain/Myeloperoxidase抗体(A-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MPO light chain/Myeloperoxidase (A-5): sc-365436免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 MPO light chain/Myeloperoxidase 抗体 (A-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MPO light chain/Myeloperoxidase 抗体 (A-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MPO轻链抗体(A-5)是一种IgG1 κ鼠单克隆MPO轻链抗体(也称为髓过氧化物酶表达中性粒细胞粒细胞标志物抗体、髓系分化过氧化物酶15 kDa轻链抗体、中性粒细胞嗜苯胺蓝颗粒标志物MPO抗体或15 kDa髓过氧化物酶(MPO)轻链亚基抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源MPO轻链蛋白。MPO轻链抗体(A-5)既可以非偶联抗MPO轻链抗体的形式提供,也可以多种偶联形式的抗MPO轻链抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。血红素蛋白髓过氧化物酶(MPO)是中性粒细胞和多形核白细胞嗜苯胺蓝颗粒的主要成分。最佳氧依赖性微生物杀菌活性取决于MPO,因为它是产生次氯酸和其他有毒氧产物的关键酶。MPO前体在髓系分化的早幼粒细胞阶段合成,随后被加工并转运到溶酶体。前体经历共翻译N-糖基化以产生糖蛋白。内质网(ER)或早期顺式高尔基体中的葡糖苷酶将前MPO转化为一种形式,该形式被分类到前溶酶体区室,该区室经历最后的蛋白水解成熟过程,形成天然MPO,即一对重-轻原聚体。在正常中性粒细胞中,MPO以二聚体的形式表达。钙网蛋白是一种驻留在ER中的钙结合蛋白,与完全糖基化的载脂蛋白前MPO特异性相互作用。iMPO mRNA在人早幼粒细胞HL-60和小鼠髓系白血病NFS-60细胞中含量丰富。MPO在循环中性粒细胞和单核细胞中高水平表达,但在小胶质细胞、脑特异性巨噬细胞或正常脑组织中检测不到。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MPO light chain/Myeloperoxidase 抗体 (A-5) 参考文献:

  1. 三种不同的髓过氧化物酶(MPO)启动子在人类髓细胞中的功能活性。  |  Lin, KM. and Austin, GE. 2002. Leukemia. 16: 1143-53. PMID: 12040446
  2. MPO-ANCA相关性血管炎患者的中性粒细胞功能。  |  Suzuki, K. 2003. Intern Med. 42: 552-3. PMID: 12879944
  3. 人类半髓过氧化物酶。在中性 pH 值下的初始氯化活性, 化合物 II 和 III 的形成,以及对次氯酸和高温的稳定性。  |  Zuurbier, KW., et al. 1992. Eur J Biochem. 205: 737-42. PMID: 1315274
  4. ANCA相关性血管炎中PR3和MPO-ANCA自身抗体特异性变化的发生率。  |  Holding, S., et al. 2015. Ann Clin Biochem. 52: 297-301. PMID: 24723621
  5. 人类髓过氧化物酶单克隆抗体的制备和特征描述。  |  Homma, T., et al. 1989. Arch Biochem Biophys. 273: 189-96. PMID: 2547339
  6. 人髓溶酶体蛋白--髓过氧化物酶的翻译后加工。  |  Nauseef, WM. 1987. Blood. 70: 1143-50. PMID: 2820530
  7. 髓过氧化物酶的生物合成与加工--髓系细胞分化的标志物  |  Nauseef, WM., et al. 1988. Eur J Haematol. 40: 97-110. PMID: 2831080
  8. 人类髓过氧化物酶 cDNA 的分子克隆和特征描述。  |  Morishita, K., et al. 1987. J Biol Chem. 262: 3844-51. PMID: 3029127
  9. 人类髓过氧化物酶 cDNA 克隆的特征:预测的氨基酸序列和多种 mRNA 的证据。  |  Johnson, KR., et al. 1987. Nucleic Acids Res. 15: 2013-28. PMID: 3031585
  10. 发现不可逆抑制髓过氧化物酶 (MPO) 的新型吲哚药层。  |  Patnaik, A., et al. 2020. Bioorg Med Chem. 28: 115548. PMID: 32503688
  11. 钙网蛋白在髓过氧化物酶的生物合成过程中起着分子伴侣的作用。  |  Nauseef, WM., et al. 1995. J Biol Chem. 270: 4741-7. PMID: 7876246
  12. 髓过氧化物酶多态性与阿尔茨海默病的特定性别风险有关。  |  Reynolds, WF., et al. 1999. Exp Neurol. 155: 31-41. PMID: 9918702

订购信息

产品名称产品编号规格价格数量收藏夹

MPO light chain/Myeloperoxidase 抗体 (A-5)

sc-365436
200 µg/ml
RMB2377.00

MPO light chain/Myeloperoxidase (A-5): m-IgG Fc BP-HRP 套装

sc-529375
200 µg Ab; 10 µg BP
RMB2662.00

MPO light chain/Myeloperoxidase (A-5): m-IgGκ BP-HRP 套装

sc-522244
200 µg Ab, 40 µg BP
RMB2662.00

MPO light chain/Myeloperoxidase 抗体 (A-5) AC

sc-365436 AC
500 µg/ml, 25% agarose
RMB3129.00

MPO light chain/Myeloperoxidase 抗体 (A-5) HRP

sc-365436 HRP
200 µg/ml
RMB2377.00

MPO light chain/Myeloperoxidase 抗体 (A-5) FITC

sc-365436 FITC
200 µg/ml
RMB2482.00

MPO light chain/Myeloperoxidase 抗体 (A-5) PE

sc-365436 PE
200 µg/ml
RMB2580.00

MPO light chain/Myeloperoxidase 抗体 (A-5) Alexa Fluor® 488

sc-365436 AF488
200 µg/ml
RMB2685.00

MPO light chain/Myeloperoxidase 抗体 (A-5) Alexa Fluor® 546

sc-365436 AF546
200 µg/ml
RMB2685.00

MPO light chain/Myeloperoxidase 抗体 (A-5) Alexa Fluor® 594

sc-365436 AF594
200 µg/ml
RMB2685.00

MPO light chain/Myeloperoxidase 抗体 (A-5) Alexa Fluor® 647

sc-365436 AF647
200 µg/ml
RMB2685.00

MPO light chain/Myeloperoxidase 抗体 (A-5) Alexa Fluor® 680

sc-365436 AF680
200 µg/ml
RMB2685.00

MPO light chain/Myeloperoxidase 抗体 (A-5) Alexa Fluor® 790

sc-365436 AF790
200 µg/ml
RMB2685.00

Is this antibody validated in FFPE human tissue? If so, what is the antigen retrieval method?

Asked by: BBCc
Thank you for your question. sc-365436 MPO light chain (A-5) is recommended for WB, IP, PAR, ELISA for human samples.
Answered by: BlakeJ
Date published: 2025-04-01

How can MPO light chain (A-5): sc-365436 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: cjMara
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-365436 HRP), phycoerythrin (sc-365436 PE), fluoroscein (sc-365436 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Specific stain in canine tissueTried this antibody on an FFPE sample of canine oral mucosa (ulcerated) and the stain was located in PMN cytoplasm, as expected.
Date published: 2020-01-30
Rated 5 out of 5 by from good featuresuse in NB4 cells ,the trips is very abvious,thank you for your free sample
Date published: 2017-09-06
Rated 5 out of 5 by from Great featurestI got this antibody two one month ago and tested it in IHC and western blot. It worked well and fit the data online.
Date published: 2016-11-24
Rated 5 out of 5 by from Strong band detected in WB in HLStrong band detected in WB in HL-60 whole cell lysate. -SCBT QC
Date published: 2015-06-21
Rated 5 out of 5 by from IF publishable data with human neutrophilIF publishable data with human neutrophil extracellular DNA nets - SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from Robust positive band seen in WB in humanRobust positive band seen in WB in human PBL whole cell lysate. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Excellent immunofluorescence cytoExcellent immunofluorescence cyto-plasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2013-06-03
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MPO light chain/Myeloperoxidase抗体 (A-5) is rated 5.0 out of 5 by 7.
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