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MHC class II 抗体 (Y-Ae): sc-32247

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说明书
  • MHC class II抗体(Y-Ae)是小鼠单克隆IgG2b λ MHC class II抗体, 在16篇文献中引用,规格为200 µg/ml
  • 针对mouse脂多糖活化的脾细胞
  • MHC class II 抗体 (Y-Ae) 推荐用于WB, IP, IF, IHC(P) 和 FCM,检测mouse 来源的 splenic B cells expressing the I-Ab MHC class II molecules complexed to the 52-68 fragment of the α chain of I-E class II molecules (the Eα 52-68 peptide)
  • 抗MHC class II抗体(Y-Ae)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MHC class II (Y-Ae): sc-32247免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成MHC class II 抗体 (Y-Ae)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

MHC class II Antibody (Y-Ae) 是一种小鼠单克隆 IgG2b 抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)检测小鼠来源的 MHC class II 蛋白。MHC II类(Y-Ae)抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。主要组织相容性复合物(MHC)II类分子通过向CD4+ T淋巴细胞呈递加工的外源抗原,在免疫反应中发挥关键作用,而CD4+ T淋巴细胞对于协调适应性免疫至关重要。这些分子主要位于树突状细胞、巨噬细胞和B细胞等抗原呈递细胞的表面,有助于识别外来肽。MHC II类分子结合并呈现13-18个氨基酸长度的肽的能力对于激活辅助性T细胞至关重要,而辅助性T细胞反过来会刺激B细胞产生抗体并增强CD8+ T细胞的细胞毒性活性。MHC II类分子与T细胞受体之间的相互作用是免疫反应的关键事件,因此抗MHC II类抗体(Y-Ae)对于理解免疫系统的动态和开发免疫疗法至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MHC class II 抗体 (Y-Ae) 参考文献:

  1. 细胞内MHC II类抗原呈递之外的自噬。  |  Münz, C. 2016. Trends Immunol. 37: 755-763. PMID: 27667710
  2. 部分MHC II类构建物作为中风的新型免疫调节疗法。  |  Benedek, G., et al. 2017. Neurochem Int. 107: 138-147. PMID: 27773790
  3. 肺癌中的MHC II类表达。  |  He, Y., et al. 2017. Lung Cancer. 112: 75-80. PMID: 29191604
  4. 造血细胞移植治疗MHC II类缺乏症。  |  Lum, SH., et al. 2019. Front Pediatr. 7: 516. PMID: 31921728
  5. 脾脏参与部分MHC II类构建物对中风神经的保护。  |  Brown, J., et al. 2020. CNS Neurosci Ther. 26: 663-669. PMID: 32237074
  6. MHC II类泛素化调节树突状细胞功能和免疫。  |  Wilson, KR., et al. 2021. J Immunol. 207: 2255-2264. PMID: 34599081
  7. MHC II 类的上皮-骨髓交换制约着免疫力和微生物群的组成。  |  Stephens, WZ., et al. 2021. Cell Rep. 37: 109916. PMID: 34731608
  8. 调节MHC II类免疫肽组可塑性的分子决定因素。  |  Santambrogio, L. 2022. Front Immunol. 13: 878271. PMID: 35651601
  9. MHC II类在自身免疫中的表现。  |  Ishina, IA., et al. 2023. Cells. 12: PMID: 36672249
  10. PAD4通过抑制巨噬细胞中的MHC II类机制来控制肿瘤免疫。  |  Pitter, MR., et al. 2024. Cell Rep. 43: 113942. PMID: 38489266

订购信息

产品名称产品编号规格价格数量收藏夹

MHC class II 抗体 (Y-Ae)

sc-32247
200 µg/ml
$316.00

MHC class II 抗体 (Y-Ae) AC

sc-32247 AC
500 µg/ml, 25% agarose
$416.00

MHC class II 抗体 (Y-Ae) HRP

sc-32247 HRP
200 µg/ml
$316.00

MHC class II 抗体 (Y-Ae) FITC

sc-32247 FITC
200 µg/ml
$330.00

MHC class II 抗体 (Y-Ae) PE

sc-32247 PE
200 µg/ml
$343.00

MHC class II 抗体 (Y-Ae) Alexa Fluor® 488

sc-32247 AF488
200 µg/ml
$357.00

MHC class II 抗体 (Y-Ae) Alexa Fluor® 546

sc-32247 AF546
200 µg/ml
$357.00

MHC class II 抗体 (Y-Ae) Alexa Fluor® 594

sc-32247 AF594
200 µg/ml
$357.00

MHC class II 抗体 (Y-Ae) Alexa Fluor® 647

sc-32247 AF647
200 µg/ml
$357.00

MHC class II 抗体 (Y-Ae) Alexa Fluor® 680

sc-32247 AF680
200 µg/ml
$357.00

MHC class II 抗体 (Y-Ae) Alexa Fluor® 790

sc-32247 AF790
200 µg/ml
$357.00

How can I save this antibody for longer?

Asked by: mengting jin
Thank you for your inquiry. All of our products are warrantied for one year from the date purchased. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service 11
Date published: 2018-01-09

Can this antibody be used with human samples?

Asked by: AbPolly
Thank you for your question. This MHC class II antibody is not recommended for use with human samples. For homology and publication information, and to discuss other available antibodies that are reactive with human, please contact our Technical Service Department.
Answered by: Technical Service
Date published: 2017-01-27
  • y_2025, m_9, d_7, h_4CST
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Rated 4 out of 5 by from Worked well in WB (brain tissue)Good specificity! I used this antibody for WB (1:750) in mouse cerebellum and prefrontal cortex.
Date published: 2022-01-26
Rated 5 out of 5 by from Produced publishable FCM data on mouse and humanStrong antibody, highly recommended for a variety of applications, several references available in the primary literature for Flow on mouse control and tumor associated macrophages and human Dental follicle.
Date published: 2016-12-29
Rated 5 out of 5 by from Good specificity in WB in 1Good specificity in WB in 1-11.15 whole cell lysate and mouse spleen tissue extract. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Published IF data with mouse splenocytesPublished IF data with mouse splenocytes - SCBT Publication Review
Date published: 2014-06-12
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MHC class II 抗体 (Y-Ae) is rated 4.8 out of 5 by 4.
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