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MHC class II Antibody (IBL-5/22) - Western Blotting - Image 16225
MHC class II 抗体 (IBL-5/22): sc-59322. 小鼠 PBL 全细胞裂解液中 MHC II 类表达的 Western 印迹分析.
MHC class II Antibody (IBL-5/22) - Western Blotting - Image 16225
MHC class II 抗体 (IBL-5/22): sc-59322. 小鼠 PBL 全细胞裂解液中 MHC II 类表达的 Western 印迹分析.
MHC class II 抗体 (IBL-5/22): sc-59322. 小鼠 PBL 全细胞裂解液中 MHC II 类表达的 Western 印迹分析.

MHC class II 抗体 (IBL-5/22): sc-59322

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寫評論提問

说明书
  • MHC class II 抗体 IBL-5/22 是大鼠单克隆 IgG1, 在22篇文献中引用，提供 100 µg/ml
  • 针对脾细胞的mouse来源
  • 推荐用于 mouse 来源的MHC class II WB, IP, IF 和 FCM检测
  • 关于如何获取MHC class II (IBL-5/22): sc-59322的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成MHC class II 抗体 (IBL-5/22)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

此外 Major Histocompatibility Complex 抗体 包括 MHC class I, MHC class I α, MHC class Ib, MHC class II, MHC class II β, MHC class II DR和MHC class II I E

MHC II 类抗体（IBL-5/22）是一种大鼠单克隆 IgG1 抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测小鼠来源的 MHC II 类。抗 MHC II 类抗体（IBL-5/22）为非结合型。主要组织相容性复合体（MHC）Ⅱ类分子在免疫反应中起着至关重要的作用，它们将处理过的外源抗原呈现给 CD4+ T 淋巴细胞，而 CD4+ T 淋巴细胞对协调适应性免疫至关重要。这些分子主要位于专业抗原递呈细胞（如树突状细胞、巨噬细胞和 B 细胞）的表面，可促进辅助性 T 细胞的活化。MHC II 类分子能够结合并呈现较长的多肽（通常为 13 至 18 个氨基酸），这对识别各种抗原至关重要，从而增强了免疫系统应对各种病原体的能力。此外，MHC II 类与协同刺激信号的相互作用对 T 细胞的活化至关重要，因此这些分子对有效的免疫监视和反应不可或缺。MHC II 类的不同结构特性，包括其 α 和 β 链的非共价结合，使其具有独特的肽结合槽，能稳定呈现的抗原，进一步强调了 MHC II 类在免疫功能中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MHC class II 抗体 (IBL-5/22) 参考文献:

  1. 在 I-Eα 缺陷小鼠体内呈现 Eα 52-68 结构相关的自身肽的功能和表型证据。  |  Viret, C. and Janeway, CA. 2000. J Immunol. 164: 4627-34. PMID: 10779766
  2. 从体外粘附的血液单核细胞中提取的小鼠树突状细胞的特征。  |  Agger, R., et al. 2000. Scand J Immunol. 52: 138-47. PMID: 10931381
  3. HLA I 类和 II 类基因及分子的多态性和进化。  |  Little, AM. and Parham, P. 1999. Rev Immunogenet. 1: 105-23. PMID: 11256568
  4. 控制 MHC 分子与肽组装的辅助蛋白质。  |  Van Kaer, L. 2001. Immunol Res. 23: 205-14. PMID: 11444385
  5. 大鼠（Rattus norvegicus）的主要组织相容性复合体。  |  Günther, E. and Walter, L. 2001. Immunogenetics. 53: 520-42. PMID: 11685465
  6. HLA 复合体的分子遗传学。  |  Fischer, GF. and Mayr, WR. 2001. Wien Klin Wochenschr. 113: 814-24. PMID: 11732117
  7. 受体介导的多肽抗原摄取可增强 MHC II 类限制反应。  |  Zaliauskiene, L., et al. 2002. J Immunol. 169: 2337-45. PMID: 12193699
  8. 关于自我的复杂性  |  Rudensky AYu,., et al. 1991. Nature. 353: 660-2. PMID: 1656278
  9. 胰腺肿瘤中的 MHC II 类表达：与瘤内炎症的联系。  |  Gaida, MM., et al. 2012. Virchows Arch. 460: 47-60. PMID: 22120497
  10. 在胸腺髓质而非皮质中表达的新型 MHC II 类表位。  |  Murphy, DB., et al. 1989. Nature. 338: 765-8. PMID: 2469959
  11. 自身免疫性疾病中呈现在 MHC II 类分子上的新自身抗原。  |  Jin, H. and Arase, H. 2024. Adv Exp Med Biol. 1444: 51-65. PMID: 38467972
  12. 揭示诱导中性粒细胞中 MHC II 类表达的信号通路。  |  Forrer, P., et al. 2024. Front Immunol. 15: 1444558. PMID: 39403371

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产品名称产品编号规格价格数量收藏夹

MHC class II 抗体 (IBL-5/22)

sc-59322
100 µg/ml
RMB2377.00

您好 请问这个抗体曝光时间大概是多少

Asked by: lan 11
Thank you for your inquiry. The exposure time should be depended on each experiment. Please contact our Technical Service Department for further questions. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。曝光时间需要根据具体实验进行调整。若后续仍然有问题请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Yanru Tao
Date published: 2022-02-10

Is it a membrane or cytoplasmic antibody?

Asked by: Anonymous
Thank you for your question. As of yet, no epitope mapping has been performed for MHC class II Antibody (IBL-5/22): sc-59322, so there is no information on whether the antibody binds the extra- or intracellular part of MHC class II.
Answered by: Tech Support Europe
Date published: 2020-08-18

which lysis buffer would be best to use while doing immunoprecipitation with this antibody?

Asked by: gagan
Thank you for your question. We recommend RIPA buffer. Here is a link to our IP protocol for your convenience: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Tech Service
Date published: 2019-04-22

what is the immunogen for sc 59322

Asked by: Bertie
Thank you for your question. This mouse monoclonal antibody was raised against spleen cells of mouse origin. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-03-04

What forms of MHC-II does this antibody recognise?

Asked by: lympho
Thank you for your question. Unfortunately we do not have this information. The exact epitope for this antibody has not been mapped, so no homology check can be performed either. Please contact Technical Service if you have any further questions.
Answered by: Tech Service
Date published: 2019-01-17

Does MHC class II Antibody (IBL-5/22): sc-59322 react with human samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 15
Date published: 2017-01-22
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Rated 5 out of 5 by from Great antibody for IF and FCGreat staining in both IF and FC using B lymphocytes (mouse cell line). Dilution 1:50. It works both before and after 4% PFA fixation.
Date published: 2019-01-10
Rated 5 out of 5 by from Strong WBVery nice western blot data; strong signal, low background
Date published: 2017-02-02
Rated 5 out of 5 by from Great Antibody!This antibody works great in western blot analysis of MHC class II expression in mouse PBL whole cell lysate.
Date published: 2017-01-11
Rated 1 out of 5 by from No bands observed with antibody for mouseNo bands observed with antibody for mouse spleen lysate.
Date published: 2015-03-26
Rated 5 out of 5 by from Good antibody data for mouse splenocytesGood antibody data for mouse splenocytes using WB and IP. -SCBT Publication Review
Date published: 2015-03-18
Rated 5 out of 5 by from Good immunocytochemistry data in mouse neuralGood immunocytochemistry data in mouse neural progenitor cells and microglia. -SCBT Publication Review
Date published: 2015-02-05
Rated 5 out of 5 by from Antibody works well for WB in C57BL/6 miceAntibody works well for WB in C57BL/6 mice peritonial macrophages. -SCBT Publication Review
Date published: 2015-01-22
Rated 5 out of 5 by from Works well for WB in mouse PBL whole cell lysateWorks well for WB in mouse PBL whole cell lysate. -SCBT QC
Date published: 2014-10-16
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MHC class II 抗体 (IBL-5/22) is rated 4.5 out of 5 by 8.
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