Date published: 2025-9-7
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MHC class II 抗体 (15G4): sc-53946

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说明书
  • MHC class II抗体(15G4)是小鼠单克隆IgG1 κ, 在4篇文献中引用，规格为200 µg/ml
  • 针对mouse来源的H-2M敲除的LPS B细胞
  • 推荐用于 mouse 来源的MHC class II molecule I-Ab bound to invariant chain (Ii) degradation intermediates p12 and class II-associated invariant chain peptides (CLIP) of mouse origin; not recommended for detection of MHC class II molecule I-Ab bound to human invariant chain degradation intermediates IP, IF 和 FCM检测; 不建议用于检测MHC class II molecule I-Ab bound to human invariant chain degradation intermediates
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是MHC class II Antibody (15G4). 的首选辅助检测试剂，这些试剂现与MHC class II Antibody (15G4) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外Major Histocompatibility Complex抗体，包括MHC class I, MHC class I α, MHC class Ib, MHC class II, MHC class II β, MHC class II DR和MHC class II I E

主要组织相容性复合体（MHC）分子，也被称为人类白细胞抗原（HLA）分子，是细胞表面的受体，能够结合外来肽并将其呈递给T淋巴细胞。MHC I类分子由两条多肽链组成，一条是α链或重链，另一条是β-2-微球蛋白，这是一种非共价结合的蛋白。细胞毒性T淋巴细胞结合由MHC I类分子呈递的抗原肽。与MHC I类分子结合的抗原通常长度为8到10个残基，并在肽结合槽中保持稳定。MHC II类分子由多态性MHC基因编码，并由α链和β链的非共价复合物组成。辅助性T淋巴细胞结合由MHC II类分子呈递的抗原肽。MHC II类分子结合13-18个氨基酸的抗原肽。HLA-DM和HLA-DO分子在内体/溶酶体区室和B细胞表面积累，通过维持有利于肽交换的构象来调节外源肽与II类分子（HLA-DR）的结合。MHC I类分子和II类分子的不同结构特性决定了它们在激活不同T淋巴细胞群中的各自作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MHC class II 抗体 (15G4) 参考文献:

  1. MHC II类表达的遗传控制。  |  Ting, JP. and Trowsdale, J. 2002. Cell. 109 Suppl: S21-33. PMID: 11983150
  2. 突触结合蛋白II负调控肥大细胞MHC II类呈递。  |  Baram, D., et al. 2002. Mol Immunol. 38: 1347-52. PMID: 12217406
  3. 研究树突状细胞中的MHC II类转运。  |  Paul, P. and Neefjes, J. 2013. Methods Mol Biol. 960: 489-507. PMID: 23329509
  4. HLA-DO及其在MHC II类抗原呈递中的作用。  |  Poluektov, YO., et al. 2013. Front Immunol. 4: 260. PMID: 24009612
  5. 小鼠MHC II类免疫肽组的高分辨率分析。  |  Sofron, A., et al. 2016. Eur J Immunol. 46: 319-28. PMID: 26495903
  6. MHC II类复合物在肽交换途径中呈现中间状态。  |  Wieczorek, M., et al. 2016. Nat Commun. 7: 13224. PMID: 27827392
  7. MHC II类分子的生物化学。  |  Giles, RC. and Capra, JD. 1985. Tissue Antigens. 25: 57-68. PMID: 3887644
  8. MHC II类分子介导的内源性抗原呈递。  |  Sant, AJ. 1994. Immunol Res. 13: 253-67. PMID: 7616053
  9. MHC II类相关不变链：在MHC II类生物合成和向CD4+ T细胞呈递抗原中发挥多种作用的分子。  |  Bertolino, P. and Rabourdin-Combe, C. 1996. Crit Rev Immunol. 16: 359-79. PMID: 8954255
  10. 人类MHC II类区域中的逆转录元件。  |  Andersson, G., et al. 1998. Trends Genet. 14: 109-14. PMID: 9540408

订购信息

产品名称产品编号规格价格数量收藏夹

MHC class II 抗体 (15G4)

sc-53946
200 µg/ml
RMB2377.00

MHC class II (15G4): m-IgG Fc BP-HRP 套装

sc-539015
200 µg Ab; 10 µg BP
RMB2662.00

MHC class II (15G4): m-IgG1 BP-HRP 套装

sc-541208
200 µg Ab; 20 µg BP
RMB2662.00

MHC class II 抗体 (15G4) FITC

sc-53946 FITC
200 µg/ml
RMB2482.00

MHC class II 抗体 (15G4) PE

sc-53946 PE
200 µg/ml
RMB2580.00

Can MHC class II (15G4): sc-53946 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MHC class II (15G4): sc-53946 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Works ok on western blotWorks on ok western blot. I cut the membrane so I do not know how non specific they are, so I am giving 4 stars which is still a very high rating.
Date published: 2017-08-01
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MHC class II 抗体 (15G4) is rated 4.0 out of 5 by 2.
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