Date published: 2025-9-8

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MGMT 抗体 (SPM287): sc-56432

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说明书
  • MGMT抗体(SPM287)是小鼠单克隆IgG1 κ, 在14篇文献中引用,规格为200 µg/ml
  • 针对human来源的纯化重组MGMT蛋白
  • 推荐用于 mouse, rat 和 human 来源的MGMT WB, IP, IF, IHC(P) 和 FCM检测
  • 关于如何获取MGMT (SPM287): sc-56432免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 MGMT 抗体 (SPM287) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与MGMT 抗体 (SPM287) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MGMT抗体(SPM287)是一种IgG1κ小鼠单克隆抗体,它通过WB、IP、IF、IHC(P)和FCM技术检测小鼠、大鼠和人类来源的MGMT。MGMT抗体(SPM287)以非偶联的抗MGMT抗体形式存在。MGMT(O6-甲基鸟嘌呤DNA甲基转移酶)是对DNA损伤作出反应而被转录激活的,并发挥修复由致癌物或细胞抑制药物引起的致突变和细胞毒性的O6-烷基鸟嘌呤损伤的功能。在p53缺陷的小鼠中,电离辐射不会诱导MGMT的产生,这表明MGMT的诱导可能需要p53。同样地,研究表明只有在表达功能性p53的细胞系中,MGMT的mRNA和蛋白质才能被电离辐射诱导产生,而在不表达野生型p53的细胞系中则不能。相反,在一项针对口腔癌细胞系的研究中,高MGMT活性与突变型p53的存在有关。类似地,与具有突变型p53的肿瘤相比,具有野生型p53的卵巢肿瘤中的MGMT活性显著降低,这支持了野生型p53下调基础MGMT启动子的观点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

MGMT 抗体 (SPM287)

sc-56432
200 µg/ml
RMB2377.00

MGMT (SPM287): m-IgG1 BP-HRP 套装

sc-531647
200 µg Ab; 20 µg BP
RMB2662.00

I would like to try a sample of Anti-MGMT Antibody (SPM287): sc-56432.

Asked by: Prof Glen Kisby
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Service
Date published: 2022-07-28

Are there any issues with using sc-56432: MGMT (SPM287) mouse monoclonal primary antibody on mouse samples?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-56432 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Great for WBThe MGMT Antibody (SPM287): sc-56432 provides good data for Western blot studies, showing clear band with minimal background noise. This antibody has also been cited multiple times for further verification.
Date published: 2017-01-24
Rated 4 out of 5 by from Published immunoperoxidase staining of humanPublished immunoperoxidase staining of human breast cancer samples. -SCBT Publication Review
Date published: 2015-04-03
Rated 3 out of 5 by from Worked well for western blotting with humanWorked well for western blotting with human MGMT transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-13
Rated 4 out of 5 by from Used for western blotting and immunohistochemicalUsed for western blotting and immunohistochemical analysis of human oral keratinocytes. -SCBT Publication Review
Date published: 2015-01-05
Rated 5 out of 5 by from StrongStrong, clean bands in Western blot analysis of MGMT expression in Jurkat and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-01-06
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MGMT 抗体 (SPM287) is rated 4.2 out of 5 by 5.
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