Date published: 2025-9-8

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MGMT 抗体 (E-1): sc-166528

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说明书
  • MGMT 抗体 E-1 是小鼠单克隆 IgG2a κ,MGMT抗体, 在9篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的MGMT的N-terminus的氨基酸9-24之间
  • MGMT 抗体 (E-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 MGMT
  • 抗MGMT抗体(E-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MGMT (E-1): sc-166528免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 MGMT 抗体 (E-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MGMT 抗体 (E-1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MGMT抗体(E-1)是一种IgG2aκ小鼠单克隆MGMT抗体,也称为甲基化DNA蛋白半胱氨酸甲基转移酶抗体,通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的MGMT蛋白。MGMT抗体(E-1)可作为非偶联的抗-MMGT抗体形式以及多种偶联形式的抗-MMT抗体获得,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。MGMT(O6甲基鸟嘌呤DNA甲基转移酶)在对DNA损伤的反应中被转录激活,并具有修复致癌物或细胞抑制药物引起的致突变和细胞毒性O6烷基鸟嘌呤损伤的功能。电离辐射诱导的MGMT在p53缺陷小鼠中不发生,这表明MGMT诱导可能需要p53。类似地,MGMT mRNA和蛋白被证明是可通过电离辐射诱导的,仅在表达功能性p53的细胞系中,而不在不表达野生型p53的细胞株中。相反,在一项对口腔癌症细胞系的研究中,高MGMT活性与突变型p53的存在相关。类似地,具有野生型p53的卵巢肿瘤中的MGMT活性显著低于具有突变型p53的肿瘤,这支持了野生型p53下调基础MGMT启动子的观点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

MGMT 抗体 (E-1)

sc-166528
200 µg/ml
RMB2377.00

MGMT (E-1): m-IgG Fc BP-HRP 套装

sc-528986
200 µg Ab; 10 µg BP
RMB2662.00

MGMT (E-1): m-IgGκ BP-HRP 套装

sc-521642
200 µg Ab, 40 µg BP
RMB2662.00

MGMT (E-1): m-IgG2a BP-HRP 套装

sc-547227
200 µg Ab; 10 µg BP
RMB2662.00

MGMT 抗体 (E-1) AC

sc-166528 AC
500 µg/ml, 25% agarose
RMB3129.00

MGMT 抗体 (E-1) HRP

sc-166528 HRP
200 µg/ml
RMB2377.00

MGMT 抗体 (E-1) FITC

sc-166528 FITC
200 µg/ml
RMB2482.00

MGMT 抗体 (E-1) PE

sc-166528 PE
200 µg/ml
RMB2580.00

MGMT 抗体 (E-1) Alexa Fluor® 488

sc-166528 AF488
200 µg/ml
RMB2685.00

MGMT 抗体 (E-1) Alexa Fluor® 546

sc-166528 AF546
200 µg/ml
RMB2685.00

MGMT 抗体 (E-1) Alexa Fluor® 594

sc-166528 AF594
200 µg/ml
RMB2685.00

MGMT 抗体 (E-1) Alexa Fluor® 647

sc-166528 AF647
200 µg/ml
RMB2685.00

MGMT 抗体 (E-1) Alexa Fluor® 680

sc-166528 AF680
200 µg/ml
RMB2685.00

MGMT 抗体 (E-1) Alexa Fluor® 790

sc-166528 AF790
200 µg/ml
RMB2685.00

MGMT (E-1) 中和勝肽

sc-166528 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-166528 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Good antibodyI can detect the staining, but I'm not sure if it's in the cytoplasm or the nucleus. Most of my staining in the cytoplasm.
Date published: 2024-09-04
Rated 5 out of 5 by from Great antibodyWe used this antibody dilluted 1:200 in immunohistrochemistry and the result showed great.
Date published: 2017-05-04
Rated 4 out of 5 by from workedOverexpressed GFP-MGMT (human clone) in 293 cells was strongly detected. there is no other bands in 293 cells. 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-03-24
Rated 5 out of 5 by from Positive results in western blot using humanPositive results in western blot using human MGMT transfected 293 whole cell lysates and MCF7 nuclear extract. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclearProduced exceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-12
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MGMT 抗体 (E-1) is rated 4.6 out of 5 by 5.
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