Date published: 2025-9-8

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MGMT 抗体 (D-4): sc-166527

5.0(3)
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说明书
  • MGMT抗体(D-4)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的MGMT的N-terminus的氨基酸9-24之间
  • 推荐用于 mouse, rat 和 human 来源的MGMT WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 MGMT (E-1): sc-166528,可查看有标记的 MGMT 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP 是 MGMT 抗体 (D-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与MGMT 抗体 (D-4) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

MGMT(O6-甲基鸟嘌呤-DNA甲基转移酶)在DNA损伤时会被转录激活,并起到修复由致癌物或细胞抑制剂引起的致突变和细胞毒性的O6-烷基鸟嘌呤损伤的作用。在p53缺陷型小鼠中,电离辐射不会诱导MGMT的产生,这表明MGMT的诱导可能需要p53的参与。同样地,研究表明,只有在表达功能性p53的细胞系中,MGMT的mRNA和蛋白质才能被电离辐射诱导产生,而在不表达野生型p53的细胞系中则不行。相反,在对口腔癌细胞系的研究中发现,高MGMT活性与突变型p53的存在有关。类似地,与具有突变型p53的肿瘤相比,具有野生型p53的卵巢肿瘤中的MGMT活性显著降低,这支持了野生型p53下调基础MGMT启动子的观点。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

MGMT 抗体 (D-4)

sc-166527
200 µg/ml
RMB2377.00

MGMT (D-4): m-IgGκ BP-HRP 套装

sc-521641
200 µg Ab, 40 µg BP
RMB2662.00

MGMT (D-4) 中和勝肽

sc-166527 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-166527 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Clean bands in western blot using MCF7 nuclearClean bands in western blot using MCF7 nuclear extracts and human MGMT transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-06-30
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-28
Rated 5 out of 5 by from CrispCrisp, clear results in western blot analysis of MGMT expression in MCF7, Jurkat and Caco-2 whole cell lysates. -SCBT QC
Date published: 2015-02-07
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MGMT 抗体 (D-4) is rated 5.0 out of 5 by 3.
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