Date published: 2025-9-8

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MGMT 抗体 (C-5): sc-271154

4.4(5)
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说明书
  • MGMT抗体(C-5)是小鼠单克隆IgG1 κ, 在4篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长MGMT的氨基酸1-207
  • 推荐用于 human 来源的MGMT WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 MGMT (E-1): sc-166528,可查看有标记的 MGMT 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 MGMT 抗体 (C-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MGMT 抗体 (C-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MGMT(O6-甲基鸟嘌呤-DNA甲基转移酶)在DNA损伤时会被转录激活,并起到修复由致癌物或细胞抑制剂引起的致突变和细胞毒性的O6-烷基鸟嘌呤损伤的作用。在p53缺陷型小鼠中,电离辐射不会诱导MGMT的产生,这表明MGMT的诱导可能需要p53的参与。同样地,研究表明,只有在表达功能性p53的细胞系中,MGMT的mRNA和蛋白质才能被电离辐射诱导产生,而在不表达野生型p53的细胞系中则不行。相反,在对口腔癌细胞系的研究中发现,高MGMT活性与突变型p53的存在有关。类似地,与具有突变型p53的肿瘤相比,具有野生型p53的卵巢肿瘤中的MGMT活性显著降低,这支持了野生型p53下调基础MGMT启动子的观点。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

MGMT 抗体 (C-5)

sc-271154
200 µg/ml
RMB2377.00

MGMT (C-5): m-IgG Fc BP-HRP 套装

sc-540214
200 µg Ab; 10 µg BP
RMB2662.00

MGMT (C-5): m-IgG1 BP-HRP 套装

sc-541976
200 µg Ab; 20 µg BP
RMB2662.00

Does this actibody react with mouse samples also?

Asked by: Isamm
Thank you for your question. sc-271154 MGMT (C-5)is recommended for human samples. The homology for mouse is 69%.
Answered by: BlakeJ
Date published: 2025-08-06

I am using MGMT (C-5): sc-271154 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent!This antibody is recommended for detection of MGMT of human origin by WB, IP, IF, IHC(P) and ELISA.
Date published: 2017-02-10
Rated 3 out of 5 by from WB with human gastric epithelial cell line GESWB with human gastric epithelial cell line GES-1, low titer but with high specific.
Date published: 2015-10-08
Rated 4 out of 5 by from Positive results in western blot using humanPositive results in western blot using human MGMT transfected 293 and MCF7 whole cell lysates. -SCBT QC
Date published: 2015-01-19
Rated 5 out of 5 by from Published western blot results using humanPublished western blot results using human and murine GBM cell lysates. -SCBT Publication Review
Date published: 2015-01-19
Rated 5 out of 5 by from Superb immunofluorescence nuclear and cytoplasmicSuperb immunofluorescence nuclear and cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2014-04-04
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MGMT 抗体 (C-5) is rated 4.4 out of 5 by 5.
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