Date published: 2025-9-6
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Mel-CAM Antibody (P1H12) - Western Blotting - Image 38267
Mel-CAM 抗体 (P1H12): sc-18837. 未转染 293T: sc-117752 (A), 转染人 Mel-CAM 的 293T: sc-116616 (B) 和 HeLa (C) 全细胞裂解液中 Mel-CAM 表达的 Western 印迹分析.
Mel-CAM 抗体 (P1H12): sc-18837. 未转染 293T: sc-117752 (A), 转染人 Mel-CAM 的 293T: sc-116616 (B) 和 HeLa (C) 全细胞裂解液中 Mel-CAM 表达的 Western 印迹分析.

Mel-CAM 抗体 (P1H12): sc-18837

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说明书
  • Mel-CAM 抗体 P1H12 是小鼠单克隆 IgG1 κ，Mel-CAM抗体, 在36篇文献中引用，规格为200 µg/ml
  • 针对human 脐带细胞
  • Mel-CAM 抗体 (P1H12) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat, human, canine 和rabbit 来源的 Mel-CAM
  • 抗Mel-CAM抗体（P1H12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mel-CAM (P1H12): sc-18837的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Mel-CAM Antibody (P1H12) for WB applications. 的首选辅助检测试剂，这些试剂现与Mel-CAM Antibody (P1H12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Mel-CAM抗体（P1H12）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测小鼠、大鼠、人、犬和兔的Mel-CAM蛋白。抗Mel-CAM抗体（P1H12）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Mel-CAM蛋白，也称为MCAM、MUC18或CD146，是一种跨膜糖蛋白，在细胞粘附中起着关键作用，特别是在黑色素瘤中。Mel-CAM的结构包括信号肽、五个免疫球蛋白样结构域、一个跨膜区域和一个短的细胞质尾部，这些结构对于钙非依赖性粘附分子的功能至关重要。Mel-CAM主要在晚期原发性和转移性黑色素瘤中表达，其表达水平与转移性疾病进展密切相关。Mel-CAM在80%的晚期原发性人类黑色素瘤中表达，因此Mel-CAM是肿瘤活性的重要标志物。Mel-CAM能够启动外向信号通路，其中涉及Fyn、FAK和paxillin等蛋白酪氨酸激酶，这对影响细胞骨架动力学至关重要，而细胞骨架动力学又会影响细胞形态和迁移。Mel-CAM对于维持胸腺结构和功能至关重要，这凸显了Mel-CAM不仅在癌症生物学中，而且在免疫系统发育中同样重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mel-CAM 抗体 (P1H12) 参考文献:

  1. CD146（Mel-CAM）在生物学和病理学中的作用。  |  Shih, IM. 1999. J Pathol. 189: 4-11. PMID: 10451481
  2. 胸腺微环境中 CD146 黏附分子（MUC18 或 MCAM）的表达。  |  Seftalioğlu, A. and Karakoç, L. 2000. Acta Histochem. 102: 69-83. PMID: 10726166
  3. 与人类内皮细胞中 CD146 参与有关的外入信号通路。  |  Anfosso, F., et al. 2001. J Biol Chem. 276: 1564-9. PMID: 11036077
  4. Mel-CAM特异性基因抑制元件通过间隙连接通信的缺失抑制黑色素瘤的生长和侵袭。  |  Satyamoorthy, K., et al. 2001. Oncogene. 20: 4676-84. PMID: 11498790
  5. 使用针对MCAM/MUC18和IL-8的全人源抗体治疗黑色素瘤的生物免疫疗法。  |  Melnikova, VO. and Bar-Eli, M. 2006. Pigment Cell Res. 19: 395-405. PMID: 16965268
  6. MUC18 是人类黑色素瘤肿瘤进展的标志物，与免疫球蛋白超家族的神经细胞粘附分子序列相似。  |  Lehmann, JM., et al. 1989. Proc Natl Acad Sci U S A. 86: 9891-5. PMID: 2602381
  7. 常见口腔癌中的Mel-CAM表达。  |  Shamloo, N., et al. 2020. J Dent (Shiraz). 21: 184-189. PMID: 33062811
  8. 宫颈癌中MEL-CAM和HSD3B1的表达。  |  Olusoji, MJ., et al. 2021. Pathol Int. 71: 561-563. PMID: 34157783
  9. MCAM+脑内皮细胞通过募集致病性CD4+ T淋巴细胞促进神经炎症。  |  Charabati, M., et al. 2023. Brain. 146: 1483-1495. PMID: 36319587
  10. Mcam通过非经典Wnt信号传导抑制巨噬细胞介导的乳腺发育。  |  Yang, X., et al. 2024. Nat Commun. 15: 36. PMID: 38167296
  11. 正常组织血管和血管肿瘤内皮上粘附分子的表达。  |  Kuzu, I., et al. 1993. Lab Invest. 69: 322-8. PMID: 7690867
  12. 黑色素瘤相关糖蛋白 MUC18 的基因组组织：对免疫球蛋白结构域进化的影响。  |  Sers, C., et al. 1993. Proc Natl Acad Sci U S A. 90: 8514-8. PMID: 8378324

订购信息

产品名称产品编号规格价格数量收藏夹

Mel-CAM 抗体 (P1H12)

sc-18837
200 µg/ml
RMB2377.00

Mel-CAM (P1H12): m-IgG Fc BP-HRP 套装

sc-526580
200 µg Ab; 10 µg BP
RMB2662.00

Mel-CAM (P1H12): m-IgG1 BP-HRP 套装

sc-531953
200 µg Ab; 20 µg BP
RMB2662.00

Mel-CAM 抗体 (P1H12) AC

sc-18837 AC
500 µg/ml, 25% agarose
RMB3129.00

Mel-CAM 抗体 (P1H12) HRP

sc-18837 HRP
200 µg/ml
RMB2377.00

Mel-CAM 抗体 (P1H12) FITC

sc-18837 FITC
200 µg/ml
RMB2482.00

Mel-CAM 抗体 (P1H12) PE

sc-18837 PE
200 µg/ml
RMB2580.00

Mel-CAM 抗体 (P1H12) Alexa Fluor® 488

sc-18837 AF488
200 µg/ml
RMB2685.00

Mel-CAM 抗体 (P1H12) Alexa Fluor® 546

sc-18837 AF546
200 µg/ml
RMB2685.00

Mel-CAM 抗体 (P1H12) Alexa Fluor® 594

sc-18837 AF594
200 µg/ml
RMB2685.00

Mel-CAM 抗体 (P1H12) Alexa Fluor® 647

sc-18837 AF647
200 µg/ml
RMB2685.00

Mel-CAM 抗体 (P1H12) Alexa Fluor® 680

sc-18837 AF680
200 µg/ml
RMB2685.00

Mel-CAM 抗体 (P1H12) Alexa Fluor® 790

sc-18837 AF790
200 µg/ml
RMB2685.00

I would like to use Mel-CAM Antibody (P1H12): sc-18837 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-18837 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-02-01
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Rated 1 out of 5 by from Did not work for western blotUsed a 1:300 dilution for WB with Sum159, Caco-2, and 293T cell lysates. No bands were observed.
Date published: 2017-11-30
Rated 4 out of 5 by from No cross-reactivity with chicken proteinsWe tested this antibody for cross-reactivity with chicken protein and no labelling could be seen (IF on frozen-sections). The company sent us a sample to test for free so this was pretty nice!
Date published: 2017-09-28
Rated 5 out of 5 by from work wellwe applicate this antibody into IHC in hPDLCs,and it works well
Date published: 2017-05-31
Rated 5 out of 5 by from Works in IF stainingWe used this antibody for IF staining in HeLa cells and got a strong intensity in the image
Date published: 2016-12-28
Rated 5 out of 5 by from Band detected at expected mw in human MelBand detected at expected mw in human Mel-CAM transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-05-23
Rated 5 out of 5 by from Published data for Flow Cytometry for usePublished data for Flow Cytometry for use in human leukocytes -SCBT Publication Review
Date published: 2015-05-16
Rated 5 out of 5 by from Published data for IF in human hMSCsPublished data for IF in human hMSCs. -SCBT Publication Review
Date published: 2015-04-04
Rated 5 out of 5 by from Good specificity and signal strength in HeLaGood specificity and signal strength in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-04
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Mel-CAM 抗体 (P1H12) is rated 4.5 out of 5 by 10.
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