Date published: 2025-9-6

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MDR1/ABCB1 抗体 (G-1): sc-13131

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说明书
  • MDR1/ABCB1抗体(G-1)是小鼠单克隆IgG2b κ, 在116篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Mdr-1的氨基酸1040-1280
  • MDR1/ABCB1 抗体 (G-1) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 MDR1; 可能与 Mdr-3产生交联反应
  • 抗MDR1/ABCB1抗体(G-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MDR1/ABCB1 (G-1): sc-13131免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是MDR1/ABCB1 抗体 (G-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与MDR1/ABCB1 抗体 (G-1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MDR1抗体(G-1)是一种小鼠单克隆IgG2b kappa轻链抗体,旨在通过靶向氨基酸1040-1280表位来检测人类MDR1蛋白。MDR1小鼠单克隆抗体(G-1)推荐用于包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)在内的应用。MDR1蛋白,也称为ABCB1、P-GP、PGY1、ABC20、CD243、GP170和p-170,作为ATP结合盒(ABC)转运蛋白发挥着至关重要的作用,它就像一个药物转运泵,将多种化疗药物主动排出细胞,从而导致了癌症治疗中观察到的多重耐药性(MDR)现象。MDR1是血脑屏障的组成部分,通过将潜在的有害物质从大脑运回血液来保护大脑。了解并检测MDR1对于研究耐药机制和制定克服各种疾病治疗挑战的策略至关重要。MDR1单克隆抗体(G-1)主要与人类MDR1反应,也可能与小鼠同源物Mdr-3交叉反应,为跨物种研究提供了额外的实用性。此外,MDR1 (G-1) 抗体有多种结合形式,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 以及各种 Alexa Fluor® 结合物,可灵活地整合到不同的实验方案中。MDR1 单克隆抗体 (G-1) 推荐用于研究耐药性和血脑屏障保护机制的研究人员。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

MDR1/ABCB1 抗体 (G-1)

sc-13131
200 µg/ml
RMB2377.00

MDR1/ABCB1 (G-1): m-IgG2b BP-HRP 套装

sc-548644
200 µg Ab; 10 µg BP
RMB2662.00

MDR1/ABCB1 抗体 (G-1) AC

sc-13131 AC
500 µg/ml, 25% agarose
RMB3129.00

MDR1/ABCB1 抗体 (G-1) HRP

sc-13131 HRP
200 µg/ml
RMB2377.00

MDR1/ABCB1 抗体 (G-1) FITC

sc-13131 FITC
200 µg/ml
RMB2482.00

MDR1/ABCB1 抗体 (G-1) PE

sc-13131 PE
200 µg/ml
RMB2580.00

MDR1/ABCB1 抗体 (G-1) Alexa Fluor® 488

sc-13131 AF488
200 µg/ml
RMB2685.00

MDR1/ABCB1 抗体 (G-1) Alexa Fluor® 546

sc-13131 AF546
200 µg/ml
RMB2685.00

MDR1/ABCB1 抗体 (G-1) Alexa Fluor® 594

sc-13131 AF594
200 µg/ml
RMB2685.00

MDR1/ABCB1 抗体 (G-1) Alexa Fluor® 647

sc-13131 AF647
200 µg/ml
RMB2685.00

MDR1/ABCB1 抗体 (G-1) Alexa Fluor® 680

sc-13131 AF680
200 µg/ml
RMB2685.00

MDR1/ABCB1 抗体 (G-1) Alexa Fluor® 790

sc-13131 AF790
200 µg/ml
RMB2685.00

Dear Santa Cruz, I would like to know if you have ever tried the MDR1 (G-1) sc 13131 antibody on mouse cells and if it works.

Asked by: Dalileh
Thank you for your question. Based on our tests with MDR1 Antibody (G-1) in cells and tissues from mouse, rat, and human, it is only recommended for detection of MDR1 of human origin by WB, IP, IF, IHC(P) and ELISA. Please refer to sc-55510 for a mouse-reactive option.
Answered by: Tech Support Europe
Date published: 2021-03-22

Can Mdr-1 (G-1): sc-13131 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, Mdr-1 (G-1): sc-13131 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-11

What secondary is best to use with Mdr-1 (G-1): sc-13131 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Mdr-1 (G-1): sc-13131, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-11
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Rated 4 out of 5 by from FunzionaA media concentrazione abbiamo iniziato ad ottenere positività in IHC su surrene e fegato umani.
Date published: 2021-08-04
Rated 5 out of 5 by from Strong signals in Western and specific staining inStrong signals in Western and specific staining in IHC
Date published: 2018-05-19
Rated 5 out of 5 by from Great Western blotTesting different specificity of Mdr-1 expression, to detect the effect is very good
Date published: 2017-08-17
Rated 5 out of 5 by from Obvious effectMdr-1 antibody worked well in the human samples, the antibody shows hight express.
Date published: 2017-05-09
Rated 5 out of 5 by from PraiseWhen work in Mdr-1 Antibody in imprinting feel good, also can be used in IP and IHC.
Date published: 2017-04-26
Rated 5 out of 5 by from Wow; superb dataJust a great antibody for multiple applications!!!
Date published: 2017-01-29
Rated 5 out of 5 by from Great for IHC(P)The Mdr-1 Antibody (G-1): sc-13131 provides excellent staining pattern for IHC applications when using formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited over 40 times for further verification.
Date published: 2017-01-12
Rated 5 out of 5 by from Working very well in Western BlotWorking very well in Western Blot. DLD-1 colon cancer cell extract was used as a positive control.
Date published: 2015-03-24
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MDR1/ABCB1 抗体 (G-1) is rated 4.9 out of 5 by 11.
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