Date published: 2025-9-7

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MCT2 抗体 (D-5): sc-166925

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说明书
  • MCT2 抗体 D-5 是小鼠单克隆 IgG2b κ,MCT2抗体, 在22篇文献中引用,规格为200 µg/ml
  • 针对氨基酸391-480的抗原,在MCT2的C-terminus处映射,mouse来源
  • MCT2 抗体 (D-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测mouse 来源的 MCT2
  • 抗MCT2抗体(D-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MCT2 (D-5): sc-166925免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 MCT2 抗体 (D-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MCT2 抗体 (D-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MCT2抗体(D-5)是一种IgG2bκ型小鼠单克隆MCT2抗体(也被称为SLC16A7抗体),它可以通过WB、IP、IF、IHC(P)和ELISA等方法检测到小鼠来源的MCT2蛋白。MCT2抗体(D-5)可以以非共轭型抗MCT2抗体形式获得,也可以以多种共轭型抗MCT2抗体形式获得,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。单羧酸类物质,如乳酸和丙酮酸,在细胞代谢中发挥着重要作用。乳酸是在糖酵解过程中大量产生的,糖酵解在正常生理条件下为细胞提供大部分ATP。然而,乳酸的积累会导致细胞内pH值下降和糖酵解停止。为了保持糖酵解的高速率进行,乳酸必须被运输出细胞。这一转运过程是由单羧酸转运体(MCTs)家族完成的,它们作为质子同向转运体,对L-乳酸具有立体选择性。MCT家族至少包含八个成员,即MCT 1-8,它们含有10-12个跨膜螺旋(TM)域,其氨基和羧基末端位于细胞质中。MCT1表达广泛,是肿瘤细胞和红细胞中主要的MCT形式。MCT2在肝脏和睾丸中高表达,而MCT3和MCT4则主要在骨骼肌中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MCT2 抗体 (D-5) 参考文献:

  1. 骨骼肌中的乳酸盐转运--单羧酸盐转运体的作用和调节。  |  Juel, C. and Halestrap, AP. 1999. J Physiol. 517 (Pt 3): 633-42. PMID: 10358105
  2. 质子连接单羧酸盐转运体(MCT)家族:结构, 功能和调节。  |  Halestrap, AP. and Price, NT. 1999. Biochem J. 343 Pt 2: 281-99. PMID: 10510291
  3. 心脏和骨骼肌中的乳酸转运体(MCT 蛋白)。  |  Bonen, A. 2000. Med Sci Sports Exerc. 32: 778-89. PMID: 10776897
  4. 心脏和快速运动骨骼肌中 MCT1 和 MCT4 的丰度和亚细胞分布。  |  Bonen, A., et al. 2000. Am J Physiol Endocrinol Metab. 278: E1067-77. PMID: 10827010
  5. 单羧酸转运体2(MCT2)作为前列腺癌的潜在生物标志物。  |  Pértega-Gomes, N., et al. 2013. Prostate. 73: 763-9. PMID: 23192371
  6. 运动诱导的小脑单羧酸转运体2的表达及其对运动表现的贡献。  |  Hoshino, D., et al. 2016. Neurosci Lett. 633: 1-6. PMID: 27619538
  7. 人类单羧酸转运体2的协同转运机制。  |  Zhang, B., et al. 2020. Nat Commun. 11: 2429. PMID: 32415067
  8. 单羧酸转运体-2在肺癌小鼠模型中的表达限制了肿瘤生长:多组学分析。  |  Khalyfa, A., et al. 2021. Int J Mol Sci. 22: PMID: 34638954
  9. 成年大鼠和哺乳大鼠脑内皮细胞和神经胶质对单羧酸盐转运体 MCT1 的表达。  |  Gerhart, DZ., et al. 1997. Am J Physiol. 273: E207-13. PMID: 9252498
  10. 与心肌缺血有关的心脏乳酸转运  |  Halestrap, AP., et al. 1997. Am J Cardiol. 80: 17A-25A. PMID: 9293952
  11. 四种新的哺乳动物单羧酸盐转运体(MCT)同源物的克隆和测序证实了一个古老的转运体家族的存在。  |  Price, NT., et al. 1998. Biochem J. 329 (Pt 2): 321-8. PMID: 9425115
  12. 人类单羧酸盐转运体 2(MCT2)是一种高亲和性丙酮酸盐转运体。  |  Lin, RY., et al. 1998. J Biol Chem. 273: 28959-65. PMID: 9786900

订购信息

产品名称产品编号规格价格数量收藏夹

MCT2 抗体 (D-5)

sc-166925
200 µg/ml
RMB2377.00

MCT2 (D-5): m-IgGκ BP-HRP 套装

sc-521801
200 µg Ab, 40 µg BP
RMB2662.00

MCT2 (D-5): m-IgG2b BP-HRP 套装

sc-548969
200 µg Ab; 10 µg BP
RMB2662.00

MCT2 抗体 (D-5) AC

sc-166925 AC
500 µg/ml, 25% agarose
RMB3129.00

MCT2 抗体 (D-5) HRP

sc-166925 HRP
200 µg/ml
RMB2377.00

MCT2 抗体 (D-5) FITC

sc-166925 FITC
200 µg/ml
RMB2482.00

MCT2 抗体 (D-5) PE

sc-166925 PE
200 µg/ml
RMB2580.00

MCT2 抗体 (D-5) Alexa Fluor® 488

sc-166925 AF488
200 µg/ml
RMB2685.00

MCT2 抗体 (D-5) Alexa Fluor® 546

sc-166925 AF546
200 µg/ml
RMB2685.00

MCT2 抗体 (D-5) Alexa Fluor® 594

sc-166925 AF594
200 µg/ml
RMB2685.00

MCT2 抗体 (D-5) Alexa Fluor® 647

sc-166925 AF647
200 µg/ml
RMB2685.00

MCT2 抗体 (D-5) Alexa Fluor® 680

sc-166925 AF680
200 µg/ml
RMB2685.00

MCT2 抗体 (D-5) Alexa Fluor® 790

sc-166925 AF790
200 µg/ml
RMB2685.00

Comercializan también el MCT2 blocking peptide? para la referencia sc-166925

Asked by: GTPA
Thank you for your question. We do not offer a blocking peptide corresponding to this particular antibody. If you are looking for a negative control, we do offer siRNA and CRISPR products for knockdown/knockout in either murine or human cells.
Answered by: Tech Support Europe
Date published: 2025-02-14

Is this antibody suitable for immunofluorescence on frozen brain slices?

Asked by: RobertaT84
Thank you for your question. This antibody is recommended for IF, or immunofluorescence on fresh or frozen tissues or cells fixed in acetone or methanol. We do not yet have published data on frozen brain tissue, but we would still warranty the antibody for that application. We do have published data in formalin-fixed paraffin-embedded tissue sections of mouse kidney, testes and stomach.
Answered by: Technical Support
Date published: 2019-03-21

I am using MCT2 (D-5): sc-166925 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good for IFThis antibody works well in my immunostaining experiment with mouse samples.
Date published: 2018-06-05
Rated 5 out of 5 by from Very specificI got free sample of this last month.It worked really well. I am going to buy full size sample soon.
Date published: 2018-04-20
Rated 1 out of 5 by from Kein Ergebnis im WBhat beim durchgeführten Westernblot kein Signal gebracht
Date published: 2018-04-03
Rated 5 out of 5 by from Great antibodyUsed for Western blot in mouse cell lines. Works great!
Date published: 2017-12-22
Rated 5 out of 5 by from Sehr gute Ergebisse im Western BlotBei der Probegröße hatten wir gute Ergebnisse im Western Blot bei einer Verdünnung von 1:100.
Date published: 2017-11-22
Rated 5 out of 5 by from excellentWe made an experiment with MCT2 Antibody antibody and found it very useful. I have a clear band and the binding is both strong and specific.
Date published: 2017-10-24
Rated 5 out of 5 by from excellentWe made an experiment with MCT2 Antibody and found it very useful. The background is clear, simple operation, short cycle, which has been widely applied.
Date published: 2017-10-23
Rated 4 out of 5 by from WB got a strong signal.We used this antibody in Western blotting with 1:200 dilution. WB got a strong signal, we are very happy about the success of the experiment
Date published: 2017-05-18
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MCT2 抗体 (D-5) is rated 4.7 out of 5 by 15.
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