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MCP-1 Antibody (ECE.2) - Western Blotting - Image 368012
MCP-1 抗体 (ECE. 2): sc-52701. 小鼠重组 MCP-1 的 Western 印迹分析.
MCP-1 Antibody (ECE.2) - Western Blotting - Image 368012
MCP-1 抗体 (ECE. 2): sc-52701. 小鼠重组 MCP-1 的 Western 印迹分析.
MCP-1 抗体 (ECE. 2): sc-52701. 小鼠重组 MCP-1 的 Western 印迹分析.

MCP-1 抗体 (ECE.2): sc-52701

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说明书
  • MCP-1 抗体 ECE.2 是大鼠单克隆 IgG1, 在31篇文献中引用，提供 100 µg/ml
  • 免疫mouse物种的MCP-1
  • 推荐用于 mouse 来源的murine MCP-1 WB, IP 和 IF检测
  • 目前，我们还没有完成MCP-1 抗体 (ECE.2)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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関連項目

额外MCP抗体，包括MCP-1, MCP-2, MCP-3和MCP-4

MCP-1抗体（ECE.2）是一种IgG1大鼠单克隆MCP-1抗体（也称为MCP-1抗体或CCL2抗体），它通过WB、IP和IF检测小鼠源性的鼠MCP-1。MCP-1抗体（ECE.2）以非共轭形式的抗MCP-1抗体形式提供。嗜酸性粒细胞趋化因子和单核细胞趋化蛋白MCP-1–5构成了C-C（或β）趋化因子的一个亚家族，其特点是一组保守的相邻半胱氨酸。MCPs由多种细胞产生，包括T淋巴细胞，这些细胞随后被IL-1、TNFα和IFN-γ等细胞因子激活。体外研究表明，MCP异构体对不同的淋巴细胞亚群表现出趋化作用。MCP-1是一种有效的嗜碱性粒细胞激活剂，但不影响嗜酸性粒细胞。MCP-1水平在感染和炎症期间会增加，而这两者都以白细胞浸润为特征。已经鉴定出两种在羧基末端有所不同的MCP-1受体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MCP-1 抗体 (ECE.2) 参考文献:

  1. 内毒素诱导的葡萄膜炎中的MCP-1表达。  |  Tuaillon, N., et al. 2002. Invest Ophthalmol Vis Sci. 43: 1493-8. PMID: 11980865
  2. 显性负MCP-1可阻止人类破骨细胞分化。  |  Morrison, NA., et al. 2014. J Cell Biochem. 115: 303-12. PMID: 23996571
  3. MCP-1 促进人类卵巢癌细胞的侵袭和粘附。  |  Furukawa, S., et al. 2013. Anticancer Res. 33: 4785-90. PMID: 24222114
  4. MCP-1-2518A/G多态性与自身免疫性疾病易感性的关系：荟萃分析  |  Chen, S., et al. 2016. Clin Rheumatol. 35: 1169-79. PMID: 26318885
  5. 内源性MCP-1/CCR2信号可刺激肾癌细胞的增殖和迁移。  |  Küper, C., et al. 2016. Oncol Lett. 12: 2201-2209. PMID: 27602164
  6. MCP-1和CCR2基因的遗传变异与IgA肾病风险。  |  Gao, J., et al. 2016. Oncotarget. 7: 77950-77957. PMID: 27788494
  7. 精神分裂症患者治疗前血清中MCP-1水平可预测对利培酮的反应。  |  Lin, Y., et al. 2017. Shanghai Arch Psychiatry. 29: 287-294. PMID: 29276352
  8. 多发性肌炎/皮肌炎患者血清MCP-1和TGF-β1的检测及其意义。  |  Wu, CY., et al. 2019. Eur J Med Res. 24: 12. PMID: 30764873
  9. MCP-1基因单倍型对精神分裂症的防护作用。  |  Saoud, H., et al. 2019. Biomed Res Int. 2019: 4042615. PMID: 31886209
  10. 系统性MCP-1水平主要来自受损肝脏，并与肝硬化并发症有关。  |  Queck, A., et al. 2020. Front Immunol. 11: 354. PMID: 32218781

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产品名称产品编号规格价格数量收藏夹

MCP-1 抗体 (ECE.2)

sc-52701
100 µg/ml
CNY2422.00

Dear Sir/Madam, Which secondary antibody can I use? Because I didn't find secondary antibody for rat IgG1. In your company just this antibody can use for checking mouse MCP-1. Thank you very much!

Asked by: ruozhi
Thank you for your question. MCP-1 Antibody (ECE.2) is a rat monoclonal IgG1. We recommend using any secondary antibody that is anti-rat IgG. Unfortunately, we do not currently offer any secondary antibodies anti-rat IgG at this time. Please check back for updates in the future!
Answered by: Technical Support
Date published: 2019-08-17

I am using MCP-1 (ECE.2): sc-52701 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by pd427 from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Antibody dilution will be 1:200 for overnight at 4oC
Date published: 2024-12-13
Rated 5 out of 5 by raindog from the antibody works great!I bought it for our mice aorta tissue staining, it works very well.
Date published: 2019-02-05
Rated 3 out of 5 by MAO123 from WB 2C12 secreted mediumIt doesn't work in the conditioned medium of C2C12 cells in Westren blotting at the diluition of 1:1000
Date published: 2018-10-05
Rated 4 out of 5 by Tanusree from Good antibodyThis antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 4 out of 5 by melody from 一般吧it is a good antibody suitable for westernblot 一般般 这个不好孵
Date published: 2017-08-30
Rated 5 out of 5 by hgmout from good antibodyVery small MW only 12kda, I recommend use 0.22um PVDF and expose long time and loading more than 60ug protein,1:200 antibody been diluted.
Date published: 2017-07-01
Rated 5 out of 5 by polulu from specific target bandThe specificity of MCP-1 antibody is very good,i have a good specific band use dilution 1:500.
Date published: 2017-05-27
Rated 5 out of 5 by creat1 from clear band seenWe observed clear band when diluted 1:500 ，Great help to us, strongly recommended for mouse brain tissue detection..
Date published: 2017-05-23
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MCP-1 抗体 (ECE.2) is rated 4.0 out of 5 by 10.
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